Coverart for item
The Resource HPLC of peptides and proteins : methods and protocols, edited by Marie-Isabel Aguilar

HPLC of peptides and proteins : methods and protocols, edited by Marie-Isabel Aguilar

Label
HPLC of peptides and proteins : methods and protocols
Title
HPLC of peptides and proteins
Title remainder
methods and protocols
Statement of responsibility
edited by Marie-Isabel Aguilar
Contributor
Subject
Language
eng
Summary
High performance liquid chromatography (HPLC) plays a critical role today in both our understanding of biological processes and in the development of peptide and protein-based pharmaceuticals. In HPLC of Peptides and Proteins: Methods and Protocols, leading experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology. These applications include proteolytic mapping, posttranslational modification, neuropeptide processing, glycopeptides and glycoproteins, MHC-binding peptides, toxins/venoms, membrane proteins, antibodies, combinatorial and proteome analysis, and enzymatic activity. Each readily reproducible protocol contains background notes, step-by-step instructions, reagent and equipment lists, and tips on both troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-follow, HPLC of Peptides and Proteins: Methods and Protocols offers every biologist and biotechnologist at all levels of expertise ready access to the powerful tools needed to develop and carry out peptide and protein separations and analyses today
Member of
Cataloging source
COO
Dewey number
572/.6536
Illustrations
illustrations
Index
index present
LC call number
QP552.P4
LC item number
H73 2004
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 68
NLM item number
  • ME9616J v.251 2004
  • H872 2004
http://library.link/vocab/relatedWorkOrContributorName
Aguilar, Marie-Isabel
Series statement
Methods in molecular biology
Series volume
251
http://library.link/vocab/subjectName
  • Peptides
  • Proteins
  • High performance liquid chromatography
  • Peptides
  • Chromatography, High Pressure Liquid
  • Proteins
  • SCIENCE
  • High performance liquid chromatography
  • Peptides
  • Proteins
Label
HPLC of peptides and proteins : methods and protocols, edited by Marie-Isabel Aguilar
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
HPLC of peptides and proteins: Basic theory and methodology / M.I. Aguilar -- Reversed-phase high-performance liquid chromatography / M.I. Aguilar -- Ion-exchange chromatography / P. Stanton -- High-performance hydrophobic interaction chromatography / K. Benedek -- Gel filtration chromatography / P. Stanton -- Hydrophilic interaction chromatography / H. Lindner and W. Helliger -- Immobilized metal ion affinity chromatography of proteins / M. Zachariou -- Immunoaffinity chromatography of proteins / S.R. Gallant -- Liquid chromatography-mass spectrometry and tandem mass spectrometry of peptides and proteins / T.L. Shen and K.R. Noon -- Capillary separations / M. Carrascal and J. Abian -- Micropreparative HPLC of peptides and proteins / E.C. Nice and M.I. Aguilar -- Multidimensional HPLC purification of proteins / E.C. Nice and M.I. Aguilar -- Analytical high-performance liquid chromatography / K. Benedek -- Prep/semiprep separations of peptides / D.B. Scanlon and J. Finlayson -- Large-scale protein chromatography / J. Bertolini, P. Gomme and P. Thomas -- Proteolytic peptide mapping / P. Hojrup -- Mass spectrometric characterization of posttranslationally modified proteins -- phosphorylation / M.R. Larsen -- Analyses of glycopeptides and glycoproteins by liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry / N. Kawasaki [and others] -- HPLC in the analysis of peptide metabolism / R.A. Lew -- Isolation and characterization of naturally processed MHC-bound peptides from the surface of antigen-presenting cells / A.W. Purcell -- Australian funnel-web spider venom analyzed with on-line RP-HPLC techniques / D. Wilson and P. Alewood -- HPLC and mass spectrometry of intrinsic membrane proteins / J.P. Whitelegge -- IgG purification / M.S. Powell and B.D. Wines -- DNA-binding proteins: LC-MS to identify key domains in rna polymerase-promoter interactions / M. Buckle -- Sensitive enzymatic analysis of histidine decarboxylase using HPLC / J. Gomez-Ramirez, I. Blanco and J. Ortiz -- Automated vs manual profiling of peptide libraries by mass spectrometry / J.L. Aubagnac [and others] -- Proteome analysis / M.J. Powell and A.T. Timperman
Control code
54760479
Dimensions
unknown
Extent
1 online resource (xiii, 413 pages)
Form of item
online
Isbn
9781592597420
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)54760479
Label
HPLC of peptides and proteins : methods and protocols, edited by Marie-Isabel Aguilar
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
HPLC of peptides and proteins: Basic theory and methodology / M.I. Aguilar -- Reversed-phase high-performance liquid chromatography / M.I. Aguilar -- Ion-exchange chromatography / P. Stanton -- High-performance hydrophobic interaction chromatography / K. Benedek -- Gel filtration chromatography / P. Stanton -- Hydrophilic interaction chromatography / H. Lindner and W. Helliger -- Immobilized metal ion affinity chromatography of proteins / M. Zachariou -- Immunoaffinity chromatography of proteins / S.R. Gallant -- Liquid chromatography-mass spectrometry and tandem mass spectrometry of peptides and proteins / T.L. Shen and K.R. Noon -- Capillary separations / M. Carrascal and J. Abian -- Micropreparative HPLC of peptides and proteins / E.C. Nice and M.I. Aguilar -- Multidimensional HPLC purification of proteins / E.C. Nice and M.I. Aguilar -- Analytical high-performance liquid chromatography / K. Benedek -- Prep/semiprep separations of peptides / D.B. Scanlon and J. Finlayson -- Large-scale protein chromatography / J. Bertolini, P. Gomme and P. Thomas -- Proteolytic peptide mapping / P. Hojrup -- Mass spectrometric characterization of posttranslationally modified proteins -- phosphorylation / M.R. Larsen -- Analyses of glycopeptides and glycoproteins by liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry / N. Kawasaki [and others] -- HPLC in the analysis of peptide metabolism / R.A. Lew -- Isolation and characterization of naturally processed MHC-bound peptides from the surface of antigen-presenting cells / A.W. Purcell -- Australian funnel-web spider venom analyzed with on-line RP-HPLC techniques / D. Wilson and P. Alewood -- HPLC and mass spectrometry of intrinsic membrane proteins / J.P. Whitelegge -- IgG purification / M.S. Powell and B.D. Wines -- DNA-binding proteins: LC-MS to identify key domains in rna polymerase-promoter interactions / M. Buckle -- Sensitive enzymatic analysis of histidine decarboxylase using HPLC / J. Gomez-Ramirez, I. Blanco and J. Ortiz -- Automated vs manual profiling of peptide libraries by mass spectrometry / J.L. Aubagnac [and others] -- Proteome analysis / M.J. Powell and A.T. Timperman
Control code
54760479
Dimensions
unknown
Extent
1 online resource (xiii, 413 pages)
Form of item
online
Isbn
9781592597420
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)54760479

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