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The Resource Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny

Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny

Label
Handbook of ELISPOT : methods and protocols
Title
Handbook of ELISPOT
Title remainder
methods and protocols
Statement of responsibility
edited by Alexander E. Kalyuzhny
Contributor
Subject
Genre
Language
eng
Summary
Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition of Handbook of ELISPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool
Member of
Cataloging source
SCB
Dewey number
616.07/9
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP519.9.E48
LC item number
H36 2012
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QW 25
NLM item number
ME9616J v.792 2012
http://library.link/vocab/relatedWorkOrContributorName
Kalyuzhny, Alexander E
Series statement
  • Springer protocols
  • Methods in molecular biology, 1940-6029
Series volume
v. 792
http://library.link/vocab/subjectName
  • Enzyme-linked immunosorbent assay
  • Enzyme-Linked Immunosorbent Assay
  • Intercellular Signaling Peptides and Proteins
  • Adaptive Immunity
  • Lymphocytes
  • Investigative Techniques
  • Cytological Techniques
  • Clinical Laboratory Techniques
  • Immunoenzyme Techniques
  • Immunosorbent Techniques
  • Biological Factors
  • Peptides
  • Proteins
  • Leukocytes, Mononuclear
  • Immunity
  • Immunoassay
  • Amino Acids, Peptides, and Proteins
  • Immune System Phenomena
  • Immunohistochemistry
  • Leukocytes
  • Blood Cells
  • Immunologic Tests
  • Molecular Probe Techniques
  • Immune System
  • Immunologic Techniques
  • Cells
  • Hemic and Immune Systems
  • Blood
  • Anatomy
  • Diagnosis
  • Cytokines
  • Enzyme-Linked Immunospot Assay
  • Methods
  • T-Lymphocytes
  • Immunity, Cellular
  • Human Anatomy & Physiology
  • Health & Biological Sciences
  • Animal Biochemistry
  • Biomedicine
  • Immunology
  • Laboratory Medicine
  • Enzyme-linked immunosorbent assay
Label
Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Unique strengths of ELISPOT for T cell diagnostics -- The impact of harmonization on ELISPOT assay performance -- Equine ELISPOT assay to study secretion of IFN[gamma] and IL-4 from peripheral blood mononuclear cells -- Utilization of feline ELISPOT for mapping vaccine epitopes -- Analyzing cellular immunity to AAV in a canine model using ELISPOT assay -- Dual- and triple-color fluorospot -- ELISPOT assay as a tool to study oxidative stress in lymphocytes -- ELISPOT assay for neuroscience research: Studying TNT[alpha] secretion from microglial cells -- ELISPOT assay as a tool to study the effects of stem cells on cytokine secretion -- Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell -- How ELISPOT morphology reflects on the productivity and kinetics of cells' secretory activity -- Mathematical algorithms for automatic search, recognition, and detection of spots in ELISPOT assay -- Objective, user-independent ELISPOT data analysis based on scientifically validated principles -- Statistical analysis of ELISPOT assays -- Response determination criteria for ELISPOT: Toward a standard that can be applied across laboratories -- Detection of vaccinia virus-specific IFN[gamma] and IL-10 secretion from human pbmcs and CD8(+) T cells by ELISPOT -- ELISPOT assays to enumerate bovine IFN-[gamma]-secreting cells for the development of novel vaccines against bovine tuberculosis -- Il-7 addition increases spot size and number as measured by T-SPOT. TB -- Overview of membranes and membrane plates used in research and diagnostic ELISPOT assays
Control code
755947843
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (x, 261 pages)
Form of item
online
Isbn
9781617793257
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-61779-325-7
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)755947843
Label
Handbook of ELISPOT : methods and protocols, edited by Alexander E. Kalyuzhny
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Unique strengths of ELISPOT for T cell diagnostics -- The impact of harmonization on ELISPOT assay performance -- Equine ELISPOT assay to study secretion of IFN[gamma] and IL-4 from peripheral blood mononuclear cells -- Utilization of feline ELISPOT for mapping vaccine epitopes -- Analyzing cellular immunity to AAV in a canine model using ELISPOT assay -- Dual- and triple-color fluorospot -- ELISPOT assay as a tool to study oxidative stress in lymphocytes -- ELISPOT assay for neuroscience research: Studying TNT[alpha] secretion from microglial cells -- ELISPOT assay as a tool to study the effects of stem cells on cytokine secretion -- Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell -- How ELISPOT morphology reflects on the productivity and kinetics of cells' secretory activity -- Mathematical algorithms for automatic search, recognition, and detection of spots in ELISPOT assay -- Objective, user-independent ELISPOT data analysis based on scientifically validated principles -- Statistical analysis of ELISPOT assays -- Response determination criteria for ELISPOT: Toward a standard that can be applied across laboratories -- Detection of vaccinia virus-specific IFN[gamma] and IL-10 secretion from human pbmcs and CD8(+) T cells by ELISPOT -- ELISPOT assays to enumerate bovine IFN-[gamma]-secreting cells for the development of novel vaccines against bovine tuberculosis -- Il-7 addition increases spot size and number as measured by T-SPOT. TB -- Overview of membranes and membrane plates used in research and diagnostic ELISPOT assays
Control code
755947843
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (x, 261 pages)
Form of item
online
Isbn
9781617793257
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-61779-325-7
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)755947843

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