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The Resource Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters, by Julie Amanda Birt, (electronic resource)

Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters, by Julie Amanda Birt, (electronic resource)

Label
Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters
Title
Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters
Statement of responsibility
by Julie Amanda Birt
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Endometriosis, a benign gynecological disease that affects millions of women, causes subfertility and pain. A rat model of surgically-induced endometriosis (Endo) was used along with surgical controls (Sham). Endo rats have embryonic anomalies which are also present in their daughters. We hypothesized that exposure to endometriosis in utero leads to short-term epigenetic modifications and long-term, heritable epigenetic changes. There was no difference in the number or quality of embryos (F1 generation) collected from Endo compared to Sham rats. Endo F1 offspring showed delayed puberty, but yielded similar numbers and quality of F2 embryos. A whole-genome microarray of transcripts from F1 embryos revealed several dysregulated signaling pathways in F1 Endo embryos including the apoptosis pathway. Cad, Parp1, Casp3 and Dnaja3 were all up-regulated in F1 Endo embryos while Nfkbia was down-regulated. Although morphologically normal, these embryos may be destined for cell death. In F2 Endo embryos there was an increase in Casp3 and Cad transcripts indicating a possible heritable misexpression. DNA methylation, a potential mechanism for these epigenetic changes, was analyzed by combined bisulfite restriction analysis for specific promoter regions of the Cad, Casp3, Parp1, and Nfkbia genes. No differential methylation was found in F1 or F2 embryos although further studies are needed. In conclusion embryonic gene expression changes in embryos from Endo rats and their daughters may lead to increased embryo apoptosis
Cataloging source
MUU
http://library.link/vocab/creatorDate
1985-
http://library.link/vocab/creatorName
Birt, Julie Amanda
Degree
M.S.
Dissertation year
2010.
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Sharpe-Timms, Kathy L.
http://library.link/vocab/subjectName
  • Endometriosis
  • Quantitative genetics
  • Gene expression
  • Rats
  • Apoptosis
  • Infertility
Target audience
specialized
Label
Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters, by Julie Amanda Birt, (electronic resource)
Instantiates
Publication
Note
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Title from PDF of title page (University of Missouri--Columbia, viewed on April 22, 2011)
  • Thesis advisor: Dr. Kathy L. Sharpe-Timms
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
714183075
Extent
1 online resource (xii, 71 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Note
Access is limited to the University of Missouri--Columbia only.
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)714183075
Label
Heritable, differential gene expression in pre-implantation embryos from rats with endometriosis and their daughters, by Julie Amanda Birt, (electronic resource)
Publication
Note
  • The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file
  • Title from PDF of title page (University of Missouri--Columbia, viewed on April 22, 2011)
  • Thesis advisor: Dr. Kathy L. Sharpe-Timms
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
714183075
Extent
1 online resource (xii, 71 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
c
Note
Access is limited to the University of Missouri--Columbia only.
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)714183075

Library Locations

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      38.944491 -92.326012
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