The Resource Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system, by Aijing Zhang

Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system, by Aijing Zhang

Label
Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system
Title
Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system
Statement of responsibility
by Aijing Zhang
Creator
Subject
Language
eng
Summary
In mouse adult skeletal muscle, Ý-myosin heavy chain (ÝMyHC) gene is primarily expressed in type I slow muscle fibers. However, its expression is down-regulated when the muscle is subjected to non-weight bearing (NWB). The ÝMyHC proximal promoter (Ý293: -293bp to +120bp) has been demonstrated to possess the full activity of the endogenous promoter throughout developmental stages, as well as responsiveness to mechanical overload (MOV) in transgenic studies. Distal muscle CAT element (dMCAT), proximal MCAT (pMCAT) and ÝA/T-rich elements have all been shown to be recognized by TEF-1 (Transcriptional Enhancer Factor-1) proteins, thus demonstrating TEF plays an important role in muscle-specific gene expression and as well as muscle plasticity. There are four members in TEF transcription factor family. Although they recognize the same DNA element, observations suggest that the functions of TEF-1 proteins are not redundant. The unique "squelching" property of TEF-1 in transfection analysis suggests that transactivation activity of TEF-1 requires other limiting and titratable proteins. Protein interactions serve as important mechanisms in transcriptional regulation. It is proposed that the transcriptional specificity of TEF-1 proteins is achieved through the association with different interacting proteins. In this study, utilizing yeast two hybrid screening we obtained two unique clones encoding proteins with known interaction with TEF proteins. The results not only demonstrate the validity of yeast two hybrid experiment in our system setting, but also shed lights on the regulation of TEF-1 transactivation activity
Additional physical form
Also issued on the Internet.
Cataloging source
MMU
http://library.link/vocab/creatorName
Zhang, Aijing
Degree
M.S.
Dissertation year
2004.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • theses
  • bibliography
http://library.link/vocab/subjectName
  • Gene Expression Regulation
  • Muscle, Skeletal
  • Trans-Activators
  • Transcription Factors
  • Transcription Factors
  • Two-Hybrid System Techniques
Label
Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system, by Aijing Zhang
Instantiates
Publication
Note
  • "May 2004"
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 70-75)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
58749891
Dimensions
28 cm
Dimensions
unknown
Extent
viii, 75 leaves
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote
Label
Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system, by Aijing Zhang
Publication
Note
  • "May 2004"
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 70-75)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
58749891
Dimensions
28 cm
Dimensions
unknown
Extent
viii, 75 leaves
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • J. Otto Lottes Health Sciences LibraryBorrow it
      1 Hospital Dr, Columbia, MO, 65201, US
      38.939544 -92.328377
Processing Feedback ...