The Resource Identification of post-synaptic receptors mediating eighth nerve function, by Shunda Renee Irons-Brown

Identification of post-synaptic receptors mediating eighth nerve function, by Shunda Renee Irons-Brown

Label
Identification of post-synaptic receptors mediating eighth nerve function
Title
Identification of post-synaptic receptors mediating eighth nerve function
Statement of responsibility
by Shunda Renee Irons-Brown
Creator
Subject
Language
eng
Summary
Glutamate is currently the primary candidate as the hair cell transmitter in the inner ear. Kainic acid (KA), a glutamate receptor agonist, has been shown to eliminate both auditory and vestibular compound action potentials (ACAPs and VCAPs) in aves. Theses and other findings support a role for glutamate/kainate receptors in afferent synaptic transmission in the avian auditory and vestibular systems. However, owing to its toxicity, KA may act indirectly on afferent terminals to block synaptic function in the auditory and vestibular periphery. In these studies, perilymphatic perfusion was used to deliver ten drugs to the inner ear synaptic receptors to more precisely identify receptors mediating auditory and vestibular CAPS in 11- to 14-day old chickens (n = 129). Perilymphatic perfusion is a novel method developed in our laboratory that allows the control of fluid parameters throughout the entire perilymphatic space of the inner ear. Dose-response curves were obtained for four drugs. DAA (D-Ü-aminoadipic acid) and dizocilpine maleate (MK-801) both NMDA-specific antagonists failed to alter VCAPs. MK-801 (1mM and 10mM) significantly reduced ACAPs. Kynurenic acid (4-hydroxyquinoline-2-carboxylic acid), a glutamate antagonist, significantly reduced only ACAPs. CNQX (6-cyano-2, 3, dihydroxy-7-nitroquinoxaline), a non-NMDA glutamate receptor antagonist, significantly reduced VCAPs only at the highest concentration tested (1mM). In contrast, CNQX reduced auditory responses at concentrations as low as 1oM. The effective concentration (EC 50 ) for ACAPs is approximately 20oM. AMPA (2-amino-3-hydroxy-5-methyl-4-isoxazoyl), a glutamate agonist, significantly reduced ACAPs only (EC 50 = 100oM). Neither Bicuculline, a GABA A receptor antagonist, nor L-NAME, a nitric oxide synthase inhibitor, altered ACAPs or VCAPs. These findings support the hypothesis that glutamate receptors mediate both auditory and vestibular responses. This work was supported by NIH R01 DC02753 (TAJ), NIH R03DC04812 (SIB), NIH Training Grant (SIB), National Organization for Hearing Research (TAJ), Thurgood Marshall Fellowship (SIB), Minority Biomedical Researchers Training Initiative (MBRTI) Fellowship (SIB)
Additional physical form
Also issued on the Internet.
Cataloging source
MMU
http://library.link/vocab/creatorDate
1974-
http://library.link/vocab/creatorName
Irons-Brown, Shunda R.
Degree
Ph. D.
Dissertation year
2002.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Receptors, Glutamate
  • Vestibulocochlear Nerve
  • Vestibulocochlear Nerve
Label
Identification of post-synaptic receptors mediating eighth nerve function, by Shunda Renee Irons-Brown
Instantiates
Publication
Note
  • "December 2002"
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 117-119)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
52511425
Dimensions
28 cm
Dimensions
unknown
Extent
ix, 120 leaves
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote
Label
Identification of post-synaptic receptors mediating eighth nerve function, by Shunda Renee Irons-Brown
Publication
Note
  • "December 2002"
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 117-119)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
52511425
Dimensions
28 cm
Dimensions
unknown
Extent
ix, 120 leaves
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • J. Otto Lottes Health Sciences LibraryBorrow it
      1 Hospital Dr, Columbia, MO, 65201, US
      38.939544 -92.328377
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