The Resource Immunocytochemical methods and protocols, edited by Lorette C. Javois
Immunocytochemical methods and protocols, edited by Lorette C. Javois
Resource Information
The item Immunocytochemical methods and protocols, edited by Lorette C. Javois represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Immunocytochemical methods and protocols, edited by Lorette C. Javois represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases
- Language
- eng
- Edition
- 2nd ed.
- Extent
- 1 online resource (xii, 465 pages)
- Contents
-
- Ute M. Kent
- Roland M. Nardone
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
- Roland M. Nardone
- In Situ RT-PCR and Hybridization Techniques
- Vishakha Thaker
- Part VII. Special Applications For the Clinical Laboratory
- Overview of the Clinical Immunohistochemistry Laboratory : Regulations and Troubleshooting Guidelines
- Patricia A. Fetsch and Andrea Abati
- Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors
- Dennis M. Frisman
- Purification of Antibodies Using Protein A-Sepharose and FPLC
- Overview of Automated Immunostainers
- Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd
- Ute M. Kent
- Conjugation of Fluorochromes to Antibodies
- Su-Yau Mao
- Biotinylation of Antibodies
- Su-Yau Mao
- Part II. Tissue Preparation for light Microscopic Analyses
- Overview of Cell Fixatives and Cell Membrane Permeants
- Melissa A. Melan
- Part I. Antibody Preparation
- Preparation of Frozen Sections for Analysis
- Gary L. Bratthauer
- Processing of Cytological Specimens
- Gary L. Bratthauer
- Processing of Tissue Culture Cells
- Gary L. Bratthauer
- Processing of Tissue Specimens
- Gary L. Bratthauer
- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
- Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- Overview of Antibody Use in Immunocytochemistry
- Part III. Light Microscopic Detection Systems
- Overview of Fluorochromes
- J. Michael Mullins
- Direct Immunofluorescent Labeling of Cells
- Lorette C. Javois
- Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells
- Mark C. Willingham
- Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells
- Mark C. Willingham
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
- Su-Yau Mao, Lorette C. Javois, and Ute M. Kent
- Lorette C. Javois
- TUNEL Assay for Apoptotic Cells
- Virginia M. Heatwole
- Overview of Fluorescence Analysis with the Confocal Microscope
- Liana Harvath
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- P. Scott Pine
- Overview of Fluorescence Photomicography
- Lorette C. Javois and J. Michael Mullins
- Overview of Antigen Detection Through Enzymatic Activity
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
- Gary L. Bratthauer
- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
- Gary L. Bratthauer
- The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods
- Gary L. Bratthauer
- Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue
- Mark Raffeld and Elaine S. Jaffe
- Multiple Antigen Immunostaining Procedures
- Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- ELISA on Attachment Dependent or Suspension Grown Cells
- Ute M. Kent
- Julie Ann Brent
- Use of Immunogold with Silver Enhancement
- Constance Oliver
- Part IV. Fluorescence Activated Cell Sorter (FACS) Analyses
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry
- Robert E. Cunningham
- Tissue Disaggregation/ Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Viable Cells
- Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Fixed Cells
- Purification of Antibodies Using Ion-Exchange Chromatography
- Robert E. Cunningham
- Fluorescent Labeling of DNA
- Robert E. Cunningham
- Deparaffinization and Processing of Pathologic Material
- Robert E. Cunningham
- Assay for Phagocytosis
- Liana Harvath and Douglas A. Terle
- Assay for Filamentous Actin
- Liana Harvath
- Assay for Chemoattractant Binding
- Ute M. Kent
- Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham
- Assay for Oxidative Metabolism
- Liana Harvath and Douglas A. Terle
- Part V. Colloidal Gold Detection Systems For Electron Microscopic Analyses
- Fixation and Embedding
- Constance Oliver and Maria Celia Jamur
- Preparation of Colloidal Gold
- Constance Oliver
- Conjugation of Colloidal Gold to Proteins
- Constance Oliver
- Purification of Antibodies Using Affinity Chromatography
- Colloidal Gold/Streptavidin Methods
- Constance Oliver
- Pre-Embedding Labeling Methods
- Constance Oliver
- Postembedding Labeling Methods
- Constance Oliver
- Part VI. Applications For Nucleic Acid Localization By In Situ Hybridization
- Overview of In Situ Hybridization
- Roland M. Nardone
- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe
- Isbn
- 9781280830211
- Label
- Immunocytochemical methods and protocols
- Title
- Immunocytochemical methods and protocols
- Statement of responsibility
- edited by Lorette C. Javois
- Subject
-
- Gidsen (vorm)
- Immunocytochemie
- Immunocytochemistry
- Immunocytochemistry
- Immunocytochemistry -- Laboratory manuals
- Immunohistochemistry -- methods
- Immunohistochemistry -- standards
- In situ hybridization
- In situ hybridization
- In situ hybridization -- Laboratory manuals
- Laboratory Manual
- Laboratory manuals
- Laboratory manuals
- SCIENCE -- Life Sciences | Anatomy & Physiology
- Wetenschappelijke technieken
- Language
- eng
- Summary
- In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases
- Cataloging source
- N$T
- Dewey number
- 571.9/64
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QR187.I45
- LC item number
- I45 1999eb
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
- QY 250
- http://library.link/vocab/relatedWorkOrContributorName
- Javois, Lorette C
- Series statement
- Methods in molecular biology
- Series volume
- v. 115
- http://library.link/vocab/subjectName
-
- Immunocytochemistry
- In situ hybridization
- Immunohistochemistry
- Immunohistochemistry
- SCIENCE
- Immunocytochemistry
- In situ hybridization
- Immunocytochemie
- Wetenschappelijke technieken
- Label
- Immunocytochemical methods and protocols, edited by Lorette C. Javois
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Ute M. Kent
- Roland M. Nardone
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
- Roland M. Nardone
- In Situ RT-PCR and Hybridization Techniques
- Vishakha Thaker
- Part VII. Special Applications For the Clinical Laboratory
- Overview of the Clinical Immunohistochemistry Laboratory : Regulations and Troubleshooting Guidelines
- Patricia A. Fetsch and Andrea Abati
- Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors
- Dennis M. Frisman
- Purification of Antibodies Using Protein A-Sepharose and FPLC
- Overview of Automated Immunostainers
- Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd
- Ute M. Kent
- Conjugation of Fluorochromes to Antibodies
- Su-Yau Mao
- Biotinylation of Antibodies
- Su-Yau Mao
- Part II. Tissue Preparation for light Microscopic Analyses
- Overview of Cell Fixatives and Cell Membrane Permeants
- Melissa A. Melan
- Part I. Antibody Preparation
- Preparation of Frozen Sections for Analysis
- Gary L. Bratthauer
- Processing of Cytological Specimens
- Gary L. Bratthauer
- Processing of Tissue Culture Cells
- Gary L. Bratthauer
- Processing of Tissue Specimens
- Gary L. Bratthauer
- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
- Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- Overview of Antibody Use in Immunocytochemistry
- Part III. Light Microscopic Detection Systems
- Overview of Fluorochromes
- J. Michael Mullins
- Direct Immunofluorescent Labeling of Cells
- Lorette C. Javois
- Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells
- Mark C. Willingham
- Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells
- Mark C. Willingham
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
- Su-Yau Mao, Lorette C. Javois, and Ute M. Kent
- Lorette C. Javois
- TUNEL Assay for Apoptotic Cells
- Virginia M. Heatwole
- Overview of Fluorescence Analysis with the Confocal Microscope
- Liana Harvath
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- P. Scott Pine
- Overview of Fluorescence Photomicography
- Lorette C. Javois and J. Michael Mullins
- Overview of Antigen Detection Through Enzymatic Activity
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
- Gary L. Bratthauer
- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
- Gary L. Bratthauer
- The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods
- Gary L. Bratthauer
- Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue
- Mark Raffeld and Elaine S. Jaffe
- Multiple Antigen Immunostaining Procedures
- Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- ELISA on Attachment Dependent or Suspension Grown Cells
- Ute M. Kent
- Julie Ann Brent
- Use of Immunogold with Silver Enhancement
- Constance Oliver
- Part IV. Fluorescence Activated Cell Sorter (FACS) Analyses
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry
- Robert E. Cunningham
- Tissue Disaggregation/ Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Viable Cells
- Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Fixed Cells
- Purification of Antibodies Using Ion-Exchange Chromatography
- Robert E. Cunningham
- Fluorescent Labeling of DNA
- Robert E. Cunningham
- Deparaffinization and Processing of Pathologic Material
- Robert E. Cunningham
- Assay for Phagocytosis
- Liana Harvath and Douglas A. Terle
- Assay for Filamentous Actin
- Liana Harvath
- Assay for Chemoattractant Binding
- Ute M. Kent
- Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham
- Assay for Oxidative Metabolism
- Liana Harvath and Douglas A. Terle
- Part V. Colloidal Gold Detection Systems For Electron Microscopic Analyses
- Fixation and Embedding
- Constance Oliver and Maria Celia Jamur
- Preparation of Colloidal Gold
- Constance Oliver
- Conjugation of Colloidal Gold to Proteins
- Constance Oliver
- Purification of Antibodies Using Affinity Chromatography
- Colloidal Gold/Streptavidin Methods
- Constance Oliver
- Pre-Embedding Labeling Methods
- Constance Oliver
- Postembedding Labeling Methods
- Constance Oliver
- Part VI. Applications For Nucleic Acid Localization By In Situ Hybridization
- Overview of In Situ Hybridization
- Roland M. Nardone
- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe
- Control code
- 44956609
- Dimensions
- unknown
- Edition
- 2nd ed.
- Extent
- 1 online resource (xii, 465 pages)
- Form of item
- online
- Isbn
- 9781280830211
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1385/1592592139.
- Other physical details
- illustrations.
- Specific material designation
- remote
- System control number
- (OCoLC)44956609
- Label
- Immunocytochemical methods and protocols, edited by Lorette C. Javois
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Ute M. Kent
- Roland M. Nardone
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
- Roland M. Nardone
- In Situ RT-PCR and Hybridization Techniques
- Vishakha Thaker
- Part VII. Special Applications For the Clinical Laboratory
- Overview of the Clinical Immunohistochemistry Laboratory : Regulations and Troubleshooting Guidelines
- Patricia A. Fetsch and Andrea Abati
- Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors
- Dennis M. Frisman
- Purification of Antibodies Using Protein A-Sepharose and FPLC
- Overview of Automated Immunostainers
- Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd
- Ute M. Kent
- Conjugation of Fluorochromes to Antibodies
- Su-Yau Mao
- Biotinylation of Antibodies
- Su-Yau Mao
- Part II. Tissue Preparation for light Microscopic Analyses
- Overview of Cell Fixatives and Cell Membrane Permeants
- Melissa A. Melan
- Part I. Antibody Preparation
- Preparation of Frozen Sections for Analysis
- Gary L. Bratthauer
- Processing of Cytological Specimens
- Gary L. Bratthauer
- Processing of Tissue Culture Cells
- Gary L. Bratthauer
- Processing of Tissue Specimens
- Gary L. Bratthauer
- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections
- Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- Overview of Antibody Use in Immunocytochemistry
- Part III. Light Microscopic Detection Systems
- Overview of Fluorochromes
- J. Michael Mullins
- Direct Immunofluorescent Labeling of Cells
- Lorette C. Javois
- Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells
- Mark C. Willingham
- Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells
- Mark C. Willingham
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
- Su-Yau Mao, Lorette C. Javois, and Ute M. Kent
- Lorette C. Javois
- TUNEL Assay for Apoptotic Cells
- Virginia M. Heatwole
- Overview of Fluorescence Analysis with the Confocal Microscope
- Liana Harvath
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- P. Scott Pine
- Overview of Fluorescence Photomicography
- Lorette C. Javois and J. Michael Mullins
- Overview of Antigen Detection Through Enzymatic Activity
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
- Gary L. Bratthauer
- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
- Gary L. Bratthauer
- The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods
- Gary L. Bratthauer
- Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue
- Mark Raffeld and Elaine S. Jaffe
- Multiple Antigen Immunostaining Procedures
- Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld
- ELISA on Attachment Dependent or Suspension Grown Cells
- Ute M. Kent
- Julie Ann Brent
- Use of Immunogold with Silver Enhancement
- Constance Oliver
- Part IV. Fluorescence Activated Cell Sorter (FACS) Analyses
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry
- Robert E. Cunningham
- Tissue Disaggregation/ Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Viable Cells
- Robert E. Cunningham
- Indirect Immunofluorescent Labeling of Fixed Cells
- Purification of Antibodies Using Ion-Exchange Chromatography
- Robert E. Cunningham
- Fluorescent Labeling of DNA
- Robert E. Cunningham
- Deparaffinization and Processing of Pathologic Material
- Robert E. Cunningham
- Assay for Phagocytosis
- Liana Harvath and Douglas A. Terle
- Assay for Filamentous Actin
- Liana Harvath
- Assay for Chemoattractant Binding
- Ute M. Kent
- Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham
- Assay for Oxidative Metabolism
- Liana Harvath and Douglas A. Terle
- Part V. Colloidal Gold Detection Systems For Electron Microscopic Analyses
- Fixation and Embedding
- Constance Oliver and Maria Celia Jamur
- Preparation of Colloidal Gold
- Constance Oliver
- Conjugation of Colloidal Gold to Proteins
- Constance Oliver
- Purification of Antibodies Using Affinity Chromatography
- Colloidal Gold/Streptavidin Methods
- Constance Oliver
- Pre-Embedding Labeling Methods
- Constance Oliver
- Postembedding Labeling Methods
- Constance Oliver
- Part VI. Applications For Nucleic Acid Localization By In Situ Hybridization
- Overview of In Situ Hybridization
- Roland M. Nardone
- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe
- Control code
- 44956609
- Dimensions
- unknown
- Edition
- 2nd ed.
- Extent
- 1 online resource (xii, 465 pages)
- Form of item
- online
- Isbn
- 9781280830211
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1385/1592592139.
- Other physical details
- illustrations.
- Specific material designation
- remote
- System control number
- (OCoLC)44956609
Subject
- Gidsen (vorm)
- Immunocytochemie
- Immunocytochemistry
- Immunocytochemistry
- Immunocytochemistry -- Laboratory manuals
- Immunohistochemistry -- methods
- Immunohistochemistry -- standards
- In situ hybridization
- In situ hybridization
- In situ hybridization -- Laboratory manuals
- Laboratory Manual
- Laboratory manuals
- Laboratory manuals
- SCIENCE -- Life Sciences | Anatomy & Physiology
- Wetenschappelijke technieken
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