The Resource Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips, by Jaya Ghosh

Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips, by Jaya Ghosh

Label
Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips
Title
Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips
Statement of responsibility
by Jaya Ghosh
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
An electrochemical microelectrode located immediately adjacent to a single neuroendocrine cell can record spikes of amperometric current that result from quantal exocytosis of oxidizable transmitter from individual vesicles. Using electroporation we have developed an efficient method where the same electrochemical microelectrode is used to electropermeabilize an adjacent chromaffin cell and then measure the consequent quantal catecholamine release using amperometry. Trains of voltage pulses, 5-7 V in amplitude and 0.1-0.2 ms in duration can reliably trigger release from cells using gold electrodes. Amperometric spikes induced by electropermeabilization have similar areas, peak heights and durations as amperometric spikes elicited by depolarizing high K+ solutions. Uptake of trypan blue stain into cells demonstrated that the plasma membrane is permeabilized by the voltage stimulus. Robust quantal release is elicited upon electroporation in 0 Ca2+/5 mM EGTA in the bath solution. Electropermeabilization-induced transmitter release requires Cl- in the bath solution--bracketed experiments demonstrate a steep dependence of the rate of electropermeabilization-induced transmitter release on [Cl- ] between 2 and 32 mM. Using the same electrochemical electrode to electroporate and record quantal release of catecholamines from an individual chromaffin cell allows precise timing of the stimulus, stimulation of a single cell at a time, and can be used to load membrane impermeant substances into a cell
Cataloging source
MUU
http://library.link/vocab/creatorDate
1982-
http://library.link/vocab/creatorName
Ghosh, Jaya
Degree
Ph.D.
Dissertation note
Dissertation
Dissertation year
2013.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Gillis, Kevin D.
Label
Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips, by Jaya Ghosh
Instantiates
Publication
Note
  • "December 2013."
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Kevin D. Gillis
  • Includes vita
Bibliography note
Includes bibliographical references (pages 110-128)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Control code
899141479
Extent
1 online resource (xi, 129 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Other physical details
illustrations (chiefly color)
Specific material designation
remote
System control number
(OCoLC)899141479
Label
Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips, by Jaya Ghosh
Publication
Note
  • "December 2013."
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Kevin D. Gillis
  • Includes vita
Bibliography note
Includes bibliographical references (pages 110-128)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Control code
899141479
Extent
1 online resource (xi, 129 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Other physical details
illustrations (chiefly color)
Specific material designation
remote
System control number
(OCoLC)899141479

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