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The Resource Ion channel localization methods and protocols, edited by Anatoli N. Lopatin and Colin G. Nichols

Ion channel localization methods and protocols, edited by Anatoli N. Lopatin and Colin G. Nichols

Label
Ion channel localization methods and protocols
Title
Ion channel localization methods and protocols
Statement of responsibility
edited by Anatoli N. Lopatin and Colin G. Nichols
Contributor
Subject
Language
eng
Summary
Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms
Member of
Action
digitized
Cataloging source
COO
Dewey number
571.6/4
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QH603.I54
LC item number
I54 2001
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • 2001 F-719
  • QH 603.I54
NLM item number
I638 2001
http://library.link/vocab/relatedWorkOrContributorName
  • Lopatin, Anatoli N
  • Nichols, Colin G
Series statement
Methods in pharmacology and toxicology
http://library.link/vocab/subjectName
  • Ion channels
  • Clinical Laboratory Techniques
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Investigative Techniques
  • Membrane Proteins
  • Carrier Proteins
  • Proteins
  • Amino Acids, Peptides, and Proteins
  • Cytological Techniques
  • Ion Channels
  • SCIENCE
  • Ion channels
  • Fluoreszenzmikroskopie
  • Grün fluoreszierendes Protein
  • Ionenkanal
  • Methode
  • Pharmakologie
Label
Ion channel localization methods and protocols, edited by Anatoli N. Lopatin and Colin G. Nichols
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • ""Ion Channel Localization""; ""Preface""; ""Contents""; ""Contributors""; ""COLOR PLATES""; ""Part I. PHARMACOLOGICAL LABELING OF ION CHANNELS AND RECEPTORS""; ""1 Fluorescent Calcium Antagonists: Tools for Imaging of L-Type Calcium Channels in Living Cells""; ""2 Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies""; ""3 GABA-A Receptors: Autoradiographic Localization of Different Ligand Sites and Identification of Subtypes of the Benzodiazepine Binding Site""; ""4 Localization of ATP P2X Receptors""
  • ""5 Small Conductance Calcium-Activated Potassium Channels in Rat Brain: Autoradiographic Localization Using Two Specific Toxins, Apamin and Scyllatoxin""""6 Mapping N-Type Calcium Channel Distributions with omega-Conotoxins""; ""7 Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development""; ""8 High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels""; ""Part II. APPLICATIONS OF GREEN FLUORESCENT PROTEIN (GFP) TECHNOLOGY""; ""9 Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells""
  • ""10 Confocal Imaging of GFP-Labeled Ion Channels""""11 Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes""; ""12 Applications of Green Fluorescent Protein (GFP) Technology: Watching Ion Channel Biogenesis in Living Cells Using GFP Fusion Constructs""; ""13 Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models""; ""14 Mutant GFP-Based FRET Analysis of K+ Channel Organization""; ""15 Delivering Ion Channels to Mammalian Cells by Membrane Fusion""
  • ""Part III. FUNCTIONAL ASSAYS IN ION CHANNEL LOCALIZATION""""16 Detection of Neurons Expressing Calcium-Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake""; ""17 Crystallization Technique for Localizing Ion Channels in Living Cells""; ""18 Imaging of Localized Neuronal Calcium Influx""; ""19 Using Caged Compounds to Map Functional Neurotransmitter Receptors""; ""Part IV. ATOMIC FORCE MICROSCOPY""; ""20 Atomic Force Microscopy of Reconstituted Ion Channels""; ""21 Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy""
  • ""22 Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy""""23 Dynamic AFM of Patch Clamped Membranes""; ""24 Localizing Ion Channels with Scanning Probe Microscopes: A Perspective""; ""Index""
Control code
123429682
Dimensions
unknown
Extent
1 online resource (xv, 502 pages)
Form of item
online
Isbn
9780896038332
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592591183
Other physical details
illustrations (some color).
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)123429682
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Ion channel localization methods and protocols, edited by Anatoli N. Lopatin and Colin G. Nichols
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • ""Ion Channel Localization""; ""Preface""; ""Contents""; ""Contributors""; ""COLOR PLATES""; ""Part I. PHARMACOLOGICAL LABELING OF ION CHANNELS AND RECEPTORS""; ""1 Fluorescent Calcium Antagonists: Tools for Imaging of L-Type Calcium Channels in Living Cells""; ""2 Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies""; ""3 GABA-A Receptors: Autoradiographic Localization of Different Ligand Sites and Identification of Subtypes of the Benzodiazepine Binding Site""; ""4 Localization of ATP P2X Receptors""
  • ""5 Small Conductance Calcium-Activated Potassium Channels in Rat Brain: Autoradiographic Localization Using Two Specific Toxins, Apamin and Scyllatoxin""""6 Mapping N-Type Calcium Channel Distributions with omega-Conotoxins""; ""7 Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development""; ""8 High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels""; ""Part II. APPLICATIONS OF GREEN FLUORESCENT PROTEIN (GFP) TECHNOLOGY""; ""9 Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells""
  • ""10 Confocal Imaging of GFP-Labeled Ion Channels""""11 Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes""; ""12 Applications of Green Fluorescent Protein (GFP) Technology: Watching Ion Channel Biogenesis in Living Cells Using GFP Fusion Constructs""; ""13 Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models""; ""14 Mutant GFP-Based FRET Analysis of K+ Channel Organization""; ""15 Delivering Ion Channels to Mammalian Cells by Membrane Fusion""
  • ""Part III. FUNCTIONAL ASSAYS IN ION CHANNEL LOCALIZATION""""16 Detection of Neurons Expressing Calcium-Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake""; ""17 Crystallization Technique for Localizing Ion Channels in Living Cells""; ""18 Imaging of Localized Neuronal Calcium Influx""; ""19 Using Caged Compounds to Map Functional Neurotransmitter Receptors""; ""Part IV. ATOMIC FORCE MICROSCOPY""; ""20 Atomic Force Microscopy of Reconstituted Ion Channels""; ""21 Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy""
  • ""22 Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy""""23 Dynamic AFM of Patch Clamped Membranes""; ""24 Localizing Ion Channels with Scanning Probe Microscopes: A Perspective""; ""Index""
Control code
123429682
Dimensions
unknown
Extent
1 online resource (xv, 502 pages)
Form of item
online
Isbn
9780896038332
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592591183
Other physical details
illustrations (some color).
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)123429682
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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