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The Resource Laboratory methods in enzymology, Volume 541 | Part C, Protein, edited by Jon Lorsch

Laboratory methods in enzymology, Volume 541 | Part C, Protein, edited by Jon Lorsch

Label
Laboratory methods in enzymology, Volume 541 | Part C, Protein
Title
Laboratory methods in enzymology
Title number
  • Volume 541
  • Part C
Title part
Protein
Statement of responsibility
edited by Jon Lorsch
Title variation
Protein
Contributor
Editor
Subject
Language
eng
Summary
In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts
Member of
Cataloging source
NLGGC
Dewey number
  • 572/.636
  • 612.0
Illustrations
illustrations
Index
index present
LC call number
QP601
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Lorsch, Jon
Series statement
Methods in enzymology,
Series volume
volume 541
http://library.link/vocab/subjectName
  • Proteins
  • Proteins
  • SCIENCE
  • Proteins
  • Proteins
Label
Laboratory methods in enzymology, Volume 541 | Part C, Protein, edited by Jon Lorsch
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Front Cover; Laboratory Methods in Enzymology: Protein Part C; Copyright; Contents; Contributors; Miscellaneous; Preface; Section I: Protein Protocols/Protein Precipitation; Chapter One: TCA Precipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 4.3. Caution; 5. Step 1A Trichloroacetic Acid Precipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 1B Deoxycholate-Trichloroacetic Acid Precipitation; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; References
  • Referenced Protocols in Methods NavigatorSection II: Protein Protocols/Protein Pull-Down Methods; Chapter Two: Coimmunoprecipitation of Proteins from Yeast; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Whole Cell Lysates; 5.1. Overview; 5.2. Duration; 6. Step 2 Normalization of Cell Lysates; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Coimmunoprecipitation; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 7.7. Tip; 7.8. Tip; 7.9. Note
  • 8. Step 4 Wash and Elute the Immunoprecipitates8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 9. Step 5 Analysis of Immunoprecipitations; 9.1. Overview; 9.2. Duration; References; Referenced Literature; Related Literature; Referenced Protocols in Methods Navigator; Chapter Three: Coupling Antibody to Cyanogen Bromide-Activated Sepharose; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Antibody and Resin; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip
  • 6. Step 2 Coupling the Antibody to the Resin6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Quench the Reaction; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Wash the Resin; 8.1. Overview; 8.2. Duration; References; Referenced Protocols in Methods Navigator; Chapter Four: Analysis of Protein-Protein Interactions by Coimmunoprecipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Isolation of the Protein of Interest by Immunoprecipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip
  • 6. Step 2 Detection of the Binding Partner by Immunoblotting6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Section III: Protein Protocols/Protein Purification; Chapter Five: Use and Application of Hydrophobic Interaction Chromatography for Protein Purification; 1. Theory; 1.1. Latest technology in HIC adsorbents; 1.2. Advantages and disadvantages of using HIC; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 3.2. Preparation; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Column Equilibration
Control code
880863858
Extent
1 online resource (xvii, 272 pages)
Form of item
online
Isbn
9781306540889
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (black and white).
Specific material designation
remote
System control number
(OCoLC)880863858
Label
Laboratory methods in enzymology, Volume 541 | Part C, Protein, edited by Jon Lorsch
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Front Cover; Laboratory Methods in Enzymology: Protein Part C; Copyright; Contents; Contributors; Miscellaneous; Preface; Section I: Protein Protocols/Protein Precipitation; Chapter One: TCA Precipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 4.3. Caution; 5. Step 1A Trichloroacetic Acid Precipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 6. Step 1B Deoxycholate-Trichloroacetic Acid Precipitation; 6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; 6.5. Tip; References
  • Referenced Protocols in Methods NavigatorSection II: Protein Protocols/Protein Pull-Down Methods; Chapter Two: Coimmunoprecipitation of Proteins from Yeast; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Whole Cell Lysates; 5.1. Overview; 5.2. Duration; 6. Step 2 Normalization of Cell Lysates; 6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Coimmunoprecipitation; 7.1. Overview; 7.2. Duration; 7.3. Tip; 7.4. Tip; 7.5. Tip; 7.6. Tip; 7.7. Tip; 7.8. Tip; 7.9. Note
  • 8. Step 4 Wash and Elute the Immunoprecipitates8.1. Overview; 8.2. Duration; 8.3. Tip; 8.4. Tip; 9. Step 5 Analysis of Immunoprecipitations; 9.1. Overview; 9.2. Duration; References; Referenced Literature; Related Literature; Referenced Protocols in Methods Navigator; Chapter Three: Coupling Antibody to Cyanogen Bromide-Activated Sepharose; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Duration; 4.2. Preparation; 5. Step 1 Preparation of Antibody and Resin; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip; 5.6. Tip
  • 6. Step 2 Coupling the Antibody to the Resin6.1. Overview; 6.2. Duration; 6.3. Tip; 7. Step 3 Quench the Reaction; 7.1. Overview; 7.2. Duration; 7.3. Tip; 8. Step 4 Wash the Resin; 8.1. Overview; 8.2. Duration; References; Referenced Protocols in Methods Navigator; Chapter Four: Analysis of Protein-Protein Interactions by Coimmunoprecipitation; 1. Theory; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Isolation of the Protein of Interest by Immunoprecipitation; 5.1. Overview; 5.2. Duration; 5.3. Tip; 5.4. Tip; 5.5. Tip
  • 6. Step 2 Detection of the Binding Partner by Immunoblotting6.1. Overview; 6.2. Duration; 6.3. Tip; 6.4. Tip; References; Referenced Literature; Referenced Protocols in Methods Navigator; Section III: Protein Protocols/Protein Purification; Chapter Five: Use and Application of Hydrophobic Interaction Chromatography for Protein Purification; 1. Theory; 1.1. Latest technology in HIC adsorbents; 1.2. Advantages and disadvantages of using HIC; 2. Equipment; 3. Materials; 3.1. Solutions and buffers; 3.2. Preparation; 4. Protocol; 4.1. Preparation; 4.2. Duration; 5. Step 1 Column Equilibration
Control code
880863858
Extent
1 online resource (xvii, 272 pages)
Form of item
online
Isbn
9781306540889
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (black and white).
Specific material designation
remote
System control number
(OCoLC)880863858

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