The Resource Matrix metalloproteinase protocols, edited by Ian M. Clark
Matrix metalloproteinase protocols, edited by Ian M. Clark
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The item Matrix metalloproteinase protocols, edited by Ian M. Clark represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Matrix metalloproteinase protocols, edited by Ian M. Clark represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories
- Language
- eng
- Extent
- 1 online resource (xv, 545 pages)
- Contents
-
- Part 1 -- MMPs and TIMPs -- an overview of the field
- Part II -- Expression and purification of MMPs and TIMPs
- Detection of MMPs and TIMPs
- Assay of MMP and TIMP activities
- Mmps and timps. An historical perspective / J.F. Woessner, Jr. -- Strategies for cloning new mmps and timps / G. Velasco and C. Lopez-Otin -- Structural studies on mmps and timps / W. Bode and K. Maskos -- Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategies / C.M. Overall -- The matrix metalloproteinase (mmp) and tissue inhibitor of metalloproteinase (timp) genes. Transcriptional and posttranscriptional regulation, signal transduction and cell-type-specific expression / M.P. Vincenti -- Models for gain-of-function and loss-of-function of mmps. Transgenic and gene targeted mice / L.M. Coussens [and others] -- Expression of mmps and timps in mammalian cells / K.M. Yeow [and others] -- Expression of recombinant matrix metalloproteinases in escherichia coli / L.J. Windsor and D.L. Steele -- Expression of human collagenase i (mmp-1) and timp-1 in a baculovirus-based expression system / R. Vallon and P. Angel -- Expression of recombinant matrix metalloproteinases in yeast / G.A. Doyle -- Expression of recombinant membrane-type mmps / M.J. Butler, M.P. d'Ortho and S.J. Atkinson -- Refolding of timp-2 from escherichia coli inclusion bodies / R.A. Williamson -- Expression and refolding of full-length human timp-1 / D. Davis -- Purification of mmps and timps / K. Shimokawa and H. Nagase -- Monitoring mmp and timp mrna expression by rt-pcr / H. Wong [and others] -- Measuring transcription of metalloproteinase genes. Nuclear run-off assay vs analysis of hnrna / A.M. Delany -- In situ hybridization for metalloproteinases and their inhibitors / T.L. Hurskainen and S.S. Apte -- Use of eia to measure mmps and timps / N. Fujimoto and K. Iwata -- Immunohistochemistry of mmps and timps / Y. Okada -- Detecting polymorphisms in mmp genes / S. Ye and A.M. Henney -- Methods for studying activation of matrix metalloproteinases / V. Knauper and G. Murphy -- Assay of matrix metalloproteinases against matrix substrates / T.E. Cawston, P. Koshy and A.D. Rowan -- Zymography and reverse zymography for detecting mmps, and timps / S.P. Hawkes, H. Li and G.T. Taniguchi -- In situ zymography / S.J. George and J.L. Johnson -- Detection of focal proteolysis using texas-red-gelatin / R.M. Hembry -- Antibodies to mmp-cleaved aggrecan / A.J. Fosang [and others] -- Cartilage proteoglycan release assay / C.J. Billington -- Immunoassay for collagenase-mediated cleavage of types i and ii collagens / R.C. Billinghurst, M. Ionescu and A.R. Poole -- Collagen degradation assays / A.P. Hollander -- Invasion assays and matrix metalloproteinases. Quantification of cellular invasion using propidium iodide labeling and confocal laser scanning microscopy / U. Benbow, K.A. Orndorff and A.L. Givan -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / G.B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases by tissue inhibitor of metalloproteinases (timp) / M. Hutton and F. Willenbrock -- Assaying growth factor activity of tissue inhibitors of metalloproteinases / K. Yamashita and T. Hayakawa
- Isbn
- 9781592590469
- Label
- Matrix metalloproteinase protocols
- Title
- Matrix metalloproteinase protocols
- Statement of responsibility
- edited by Ian M. Clark
- Subject
-
- Extracellulaire matrix
- Extracellular Matrix Proteins
- Extracellular matrix proteins
- Extracellular matrix proteins
- Extracellular matrix proteins -- Laboratory manuals
- Grundsubstanz
- Handbooks, manuals, etc
- Laboratoriumonderzoek
- Laboratory Manual
- Laboratory manuals
- Laboratory manuals
- Matrix Metalloproteinases
- Metalloproteinasen
- Metalloproteinases
- Metalloproteinases
- Metalloproteinases -- Laboratory manuals
- Metalloproteïnases
- Metaloproteinases
- Methode
- Molekularbiologie
- SCIENCE -- Life Sciences | Biochemistry
- Language
- eng
- Summary
- From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories
- Action
- digitized
- Cataloging source
- COO
- Dewey number
- 572/.76
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QP601.7
- LC item number
- .M38 2001
- Literary form
- non fiction
- NAL call number
- QH506
- NAL item number
- .M45 v. 151
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
-
- W1
- QU 25
- NLM item number
-
- ME9616J v.151 2001
- M433 2001
- http://library.link/vocab/relatedWorkOrContributorName
- Clark, Ian M
- Series statement
- Methods in molecular biology
- Series volume
- 151
- http://library.link/vocab/subjectName
-
- Metalloproteinases
- Extracellular matrix proteins
- Matrix Metalloproteinases
- Extracellular Matrix Proteins
- SCIENCE
- Extracellular matrix proteins
- Metalloproteinases
- Metalloproteïnases
- Extracellulaire matrix
- Laboratoriumonderzoek
- Metalloproteinasen
- Grundsubstanz
- Molekularbiologie
- Methode
- Metaloproteinases
- Label
- Matrix metalloproteinase protocols, edited by Ian M. Clark
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Part 1 -- MMPs and TIMPs -- an overview of the field
- Part II -- Expression and purification of MMPs and TIMPs
- Detection of MMPs and TIMPs
- Assay of MMP and TIMP activities
- Mmps and timps. An historical perspective / J.F. Woessner, Jr. -- Strategies for cloning new mmps and timps / G. Velasco and C. Lopez-Otin -- Structural studies on mmps and timps / W. Bode and K. Maskos -- Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategies / C.M. Overall -- The matrix metalloproteinase (mmp) and tissue inhibitor of metalloproteinase (timp) genes. Transcriptional and posttranscriptional regulation, signal transduction and cell-type-specific expression / M.P. Vincenti -- Models for gain-of-function and loss-of-function of mmps. Transgenic and gene targeted mice / L.M. Coussens [and others] -- Expression of mmps and timps in mammalian cells / K.M. Yeow [and others] -- Expression of recombinant matrix metalloproteinases in escherichia coli / L.J. Windsor and D.L. Steele -- Expression of human collagenase i (mmp-1) and timp-1 in a baculovirus-based expression system / R. Vallon and P. Angel -- Expression of recombinant matrix metalloproteinases in yeast / G.A. Doyle -- Expression of recombinant membrane-type mmps / M.J. Butler, M.P. d'Ortho and S.J. Atkinson -- Refolding of timp-2 from escherichia coli inclusion bodies / R.A. Williamson -- Expression and refolding of full-length human timp-1 / D. Davis -- Purification of mmps and timps / K. Shimokawa and H. Nagase -- Monitoring mmp and timp mrna expression by rt-pcr / H. Wong [and others] -- Measuring transcription of metalloproteinase genes. Nuclear run-off assay vs analysis of hnrna / A.M. Delany -- In situ hybridization for metalloproteinases and their inhibitors / T.L. Hurskainen and S.S. Apte -- Use of eia to measure mmps and timps / N. Fujimoto and K. Iwata -- Immunohistochemistry of mmps and timps / Y. Okada -- Detecting polymorphisms in mmp genes / S. Ye and A.M. Henney -- Methods for studying activation of matrix metalloproteinases / V. Knauper and G. Murphy -- Assay of matrix metalloproteinases against matrix substrates / T.E. Cawston, P. Koshy and A.D. Rowan -- Zymography and reverse zymography for detecting mmps, and timps / S.P. Hawkes, H. Li and G.T. Taniguchi -- In situ zymography / S.J. George and J.L. Johnson -- Detection of focal proteolysis using texas-red-gelatin / R.M. Hembry -- Antibodies to mmp-cleaved aggrecan / A.J. Fosang [and others] -- Cartilage proteoglycan release assay / C.J. Billington -- Immunoassay for collagenase-mediated cleavage of types i and ii collagens / R.C. Billinghurst, M. Ionescu and A.R. Poole -- Collagen degradation assays / A.P. Hollander -- Invasion assays and matrix metalloproteinases. Quantification of cellular invasion using propidium iodide labeling and confocal laser scanning microscopy / U. Benbow, K.A. Orndorff and A.L. Givan -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / G.B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases by tissue inhibitor of metalloproteinases (timp) / M. Hutton and F. Willenbrock -- Assaying growth factor activity of tissue inhibitors of metalloproteinases / K. Yamashita and T. Hayakawa
- Control code
- 170167015
- Dimensions
- unknown
- Extent
- 1 online resource (xv, 545 pages)
- Form of item
- online
- Isbn
- 9781592590469
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1385/1592590462
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic reproduction.
- Specific material designation
- remote
- System control number
- (OCoLC)170167015
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
- Label
- Matrix metalloproteinase protocols, edited by Ian M. Clark
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Part 1 -- MMPs and TIMPs -- an overview of the field
- Part II -- Expression and purification of MMPs and TIMPs
- Detection of MMPs and TIMPs
- Assay of MMP and TIMP activities
- Mmps and timps. An historical perspective / J.F. Woessner, Jr. -- Strategies for cloning new mmps and timps / G. Velasco and C. Lopez-Otin -- Structural studies on mmps and timps / W. Bode and K. Maskos -- Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategies / C.M. Overall -- The matrix metalloproteinase (mmp) and tissue inhibitor of metalloproteinase (timp) genes. Transcriptional and posttranscriptional regulation, signal transduction and cell-type-specific expression / M.P. Vincenti -- Models for gain-of-function and loss-of-function of mmps. Transgenic and gene targeted mice / L.M. Coussens [and others] -- Expression of mmps and timps in mammalian cells / K.M. Yeow [and others] -- Expression of recombinant matrix metalloproteinases in escherichia coli / L.J. Windsor and D.L. Steele -- Expression of human collagenase i (mmp-1) and timp-1 in a baculovirus-based expression system / R. Vallon and P. Angel -- Expression of recombinant matrix metalloproteinases in yeast / G.A. Doyle -- Expression of recombinant membrane-type mmps / M.J. Butler, M.P. d'Ortho and S.J. Atkinson -- Refolding of timp-2 from escherichia coli inclusion bodies / R.A. Williamson -- Expression and refolding of full-length human timp-1 / D. Davis -- Purification of mmps and timps / K. Shimokawa and H. Nagase -- Monitoring mmp and timp mrna expression by rt-pcr / H. Wong [and others] -- Measuring transcription of metalloproteinase genes. Nuclear run-off assay vs analysis of hnrna / A.M. Delany -- In situ hybridization for metalloproteinases and their inhibitors / T.L. Hurskainen and S.S. Apte -- Use of eia to measure mmps and timps / N. Fujimoto and K. Iwata -- Immunohistochemistry of mmps and timps / Y. Okada -- Detecting polymorphisms in mmp genes / S. Ye and A.M. Henney -- Methods for studying activation of matrix metalloproteinases / V. Knauper and G. Murphy -- Assay of matrix metalloproteinases against matrix substrates / T.E. Cawston, P. Koshy and A.D. Rowan -- Zymography and reverse zymography for detecting mmps, and timps / S.P. Hawkes, H. Li and G.T. Taniguchi -- In situ zymography / S.J. George and J.L. Johnson -- Detection of focal proteolysis using texas-red-gelatin / R.M. Hembry -- Antibodies to mmp-cleaved aggrecan / A.J. Fosang [and others] -- Cartilage proteoglycan release assay / C.J. Billington -- Immunoassay for collagenase-mediated cleavage of types i and ii collagens / R.C. Billinghurst, M. Ionescu and A.R. Poole -- Collagen degradation assays / A.P. Hollander -- Invasion assays and matrix metalloproteinases. Quantification of cellular invasion using propidium iodide labeling and confocal laser scanning microscopy / U. Benbow, K.A. Orndorff and A.L. Givan -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / G.B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases by tissue inhibitor of metalloproteinases (timp) / M. Hutton and F. Willenbrock -- Assaying growth factor activity of tissue inhibitors of metalloproteinases / K. Yamashita and T. Hayakawa
- Control code
- 170167015
- Dimensions
- unknown
- Extent
- 1 online resource (xv, 545 pages)
- Form of item
- online
- Isbn
- 9781592590469
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1385/1592590462
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic reproduction.
- Specific material designation
- remote
- System control number
- (OCoLC)170167015
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Subject
- Extracellulaire matrix
- Extracellular Matrix Proteins
- Extracellular matrix proteins
- Extracellular matrix proteins
- Extracellular matrix proteins -- Laboratory manuals
- Grundsubstanz
- Handbooks, manuals, etc
- Laboratoriumonderzoek
- Laboratory Manual
- Laboratory manuals
- Laboratory manuals
- Matrix Metalloproteinases
- Metalloproteinasen
- Metalloproteinases
- Metalloproteinases
- Metalloproteinases -- Laboratory manuals
- Metalloproteïnases
- Metaloproteinases
- Methode
- Molekularbiologie
- SCIENCE -- Life Sciences | Biochemistry
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