Coverart for item
The Resource Metabolons and supramolecular enzyme assemblies, edited by Claudia Schmidt-Dannert, Maureen B. Quin

Metabolons and supramolecular enzyme assemblies, edited by Claudia Schmidt-Dannert, Maureen B. Quin

Label
Metabolons and supramolecular enzyme assemblies
Title
Metabolons and supramolecular enzyme assemblies
Statement of responsibility
edited by Claudia Schmidt-Dannert, Maureen B. Quin
Contributor
Editor
Subject
Language
eng
Member of
Cataloging source
N$T
Dewey number
660.6/34
Index
no index present
LC call number
TP248.65.E59
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Schmidt-Dannert, Claudia
  • Quin, Maureen B.
Series statement
Methods in enzymology
Series volume
volume 617
http://library.link/vocab/subjectName
  • Enzymes
  • Enzymology
  • Supramolecular chemistry
  • Enzymes
  • Metabolomics
  • Metabolic Networks and Pathways
  • Metabolome
  • Molecular Biology
  • SCIENCE
  • TECHNOLOGY & ENGINEERING
  • Enzymes
  • Enzymology
  • Supramolecular chemistry
Label
Metabolons and supramolecular enzyme assemblies, edited by Claudia Schmidt-Dannert, Maureen B. Quin
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Front Cover; Metabolons and Supramolecular Enzyme Assemblies; Copyright; Contents; Contributors; Preface; Chapter One: Molecular snapshots of dynamic membrane-bound metabolons; 1. Introduction; 2. SMA copolymer preparation; 2.1. SMA copolymer hydrolysis; 2.1.1. Equipment; 2.1.2. Buffers and reagents; 2.1.3. Procedures; 2.1.4. Notes; 3. Preparation of S. bicolor microsomes; 3.1. Growth of etiolated S. bicolor seedlings; 3.1.1. Equipment; 3.1.2. Buffers and reagents; 3.1.3. Procedures; 3.1.4. Notes; 3.2. Preparation of microsomes; 3.2.1. Equipment; 3.2.2. Buffers and reagents; 3.2.3. Procedures
  • 3.2.4. Notes4. Isolation of membrane-bound metabolons from native membranes; 4.1. SMALP preparation from sorghum microsomes; 4.1.1. Equipment; 4.1.2. Buffers and reagents; 4.1.3. Procedures; 4.1.4. Notes; 4.2. Purification of SMALPs containing a target protein; 4.2.1. Equipment; 4.2.2. Buffers and reagents; 4.2.3. Procedures; 4.2.4. Notes; 5. Characterization of SMALPs containing P450 metabolons; 5.1. SMALPs observed with electron microscope; 5.1.1. Equipment; 5.1.2. Buffers and reagents; 5.1.3. Procedures; 5.1.4. Notes; 6. Summary and conclusion; References
  • Chapter Two: Tricarboxylic acid metabolon1. Introduction; 1.1. Importance of the TCA metabolon; 1.2. Early evidence of the TCA metabolon and challenges associated with its study; 1.3. Cross-linking mass spectrometric analysis of the TCA metabolon; 2. Preparation of cross-linked native TCA metabolon; 2.1. Isolation of intact mitochondria; 2.2. In situ cross-linking; 2.3. Isolation and characterization of metabolons; 3. Preparation of recombinant TCA metabolon; 3.1. Gene construction for recombinant TCA enzymes; 3.2. Overexpression and purification of recombinant enzymes
  • 3.3. In-solution cross-linking and isolation of recombinant metabolons4. Mass spectrometric analysis of the Krebs cycle metabolon; 4.1. In-gel digestion of metabolons; 4.2. Mass spectrometric analysis; 4.3. Mascot database searches; 4.4. Structural reconstitution of the Krebs cycle metabolon; 5. Cross-linking techniques have allowed for the isolation of the intact metabolon; 6. Conclusions; References; Chapter Three: Metabolon formation by chemotaxis; 1. Introduction; 1.1. Metabolon formation; 1.2. Enzyme diffusion; 1.3. Enzyme chemotaxis; 2. Chemotactic assembly of enzyme cascade
  • 2.1. Chemotactic assembly in the microfluidic channel2.1.1. Fluorescently labeling enzymes and activity assay; 2.1.2. Microfluidic device fabrication; 2.1.3. Confocal microscope imaging; 2.1.4. Chemotactic migration of hexokinase and aldolase in the microfluidic device; 2.2. Chemotactic colocalization of hexokinase and aldolase; 2.2.1. Colocalization setup; 2.2.2. Analysis of HK and Ald aggregates trajectories; 3. Summary; Acknowledgments; References; Chapter Four: Cloning, expression, and purification of intact polyketide synthase modules; 1. Introduction; 2. Cloning of intact PKS modules
Control code
1088407326
Dimensions
unknown
Edition
First edition.
Extent
1 online resource
File format
unknown
Form of item
online
Isbn
9780128170755
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
http://library.link/vocab/ext/overdrive/overdriveId
9780128170755
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)1088407326
Label
Metabolons and supramolecular enzyme assemblies, edited by Claudia Schmidt-Dannert, Maureen B. Quin
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Front Cover; Metabolons and Supramolecular Enzyme Assemblies; Copyright; Contents; Contributors; Preface; Chapter One: Molecular snapshots of dynamic membrane-bound metabolons; 1. Introduction; 2. SMA copolymer preparation; 2.1. SMA copolymer hydrolysis; 2.1.1. Equipment; 2.1.2. Buffers and reagents; 2.1.3. Procedures; 2.1.4. Notes; 3. Preparation of S. bicolor microsomes; 3.1. Growth of etiolated S. bicolor seedlings; 3.1.1. Equipment; 3.1.2. Buffers and reagents; 3.1.3. Procedures; 3.1.4. Notes; 3.2. Preparation of microsomes; 3.2.1. Equipment; 3.2.2. Buffers and reagents; 3.2.3. Procedures
  • 3.2.4. Notes4. Isolation of membrane-bound metabolons from native membranes; 4.1. SMALP preparation from sorghum microsomes; 4.1.1. Equipment; 4.1.2. Buffers and reagents; 4.1.3. Procedures; 4.1.4. Notes; 4.2. Purification of SMALPs containing a target protein; 4.2.1. Equipment; 4.2.2. Buffers and reagents; 4.2.3. Procedures; 4.2.4. Notes; 5. Characterization of SMALPs containing P450 metabolons; 5.1. SMALPs observed with electron microscope; 5.1.1. Equipment; 5.1.2. Buffers and reagents; 5.1.3. Procedures; 5.1.4. Notes; 6. Summary and conclusion; References
  • Chapter Two: Tricarboxylic acid metabolon1. Introduction; 1.1. Importance of the TCA metabolon; 1.2. Early evidence of the TCA metabolon and challenges associated with its study; 1.3. Cross-linking mass spectrometric analysis of the TCA metabolon; 2. Preparation of cross-linked native TCA metabolon; 2.1. Isolation of intact mitochondria; 2.2. In situ cross-linking; 2.3. Isolation and characterization of metabolons; 3. Preparation of recombinant TCA metabolon; 3.1. Gene construction for recombinant TCA enzymes; 3.2. Overexpression and purification of recombinant enzymes
  • 3.3. In-solution cross-linking and isolation of recombinant metabolons4. Mass spectrometric analysis of the Krebs cycle metabolon; 4.1. In-gel digestion of metabolons; 4.2. Mass spectrometric analysis; 4.3. Mascot database searches; 4.4. Structural reconstitution of the Krebs cycle metabolon; 5. Cross-linking techniques have allowed for the isolation of the intact metabolon; 6. Conclusions; References; Chapter Three: Metabolon formation by chemotaxis; 1. Introduction; 1.1. Metabolon formation; 1.2. Enzyme diffusion; 1.3. Enzyme chemotaxis; 2. Chemotactic assembly of enzyme cascade
  • 2.1. Chemotactic assembly in the microfluidic channel2.1.1. Fluorescently labeling enzymes and activity assay; 2.1.2. Microfluidic device fabrication; 2.1.3. Confocal microscope imaging; 2.1.4. Chemotactic migration of hexokinase and aldolase in the microfluidic device; 2.2. Chemotactic colocalization of hexokinase and aldolase; 2.2.1. Colocalization setup; 2.2.2. Analysis of HK and Ald aggregates trajectories; 3. Summary; Acknowledgments; References; Chapter Four: Cloning, expression, and purification of intact polyketide synthase modules; 1. Introduction; 2. Cloning of intact PKS modules
Control code
1088407326
Dimensions
unknown
Edition
First edition.
Extent
1 online resource
File format
unknown
Form of item
online
Isbn
9780128170755
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
http://library.link/vocab/ext/overdrive/overdriveId
9780128170755
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)1088407326

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