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The Resource Microsatellites : methods and protocols, edited by Stella K. Kantartzi

Microsatellites : methods and protocols, edited by Stella K. Kantartzi

Label
Microsatellites : methods and protocols
Title
Microsatellites
Title remainder
methods and protocols
Statement of responsibility
edited by Stella K. Kantartzi
Contributor
Subject
Genre
Language
eng
Summary
Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology[trade mark symbol] series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis -- P. 4 of cover
Member of
Cataloging source
NLM
Illustrations
illustrations
Index
index present
LC call number
  • QH452.2
  • QH506
LC item number
  • .M537 2013
  • .M45 v.1006
Literary form
non fiction
Nature of contents
bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.1006 2013
http://library.link/vocab/relatedWorkOrContributorName
Kantartzi, Stella K
Series statement
  • Methods in molecular biology,
  • Springer protocols
Series volume
v. 1006
http://library.link/vocab/subjectName
  • Microsatellites (Genetics)
  • Microsatellite Repeats
  • Methode
  • Satelliten-DNS
Label
Microsatellites : methods and protocols, edited by Stella K. Kantartzi
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
1. Microsatellites : evolution and contribution / Panagiotis Madesis, Ioannis Ganopoulos, and Athanasios Tsaftaris -- 2. Screening of genomic libraries / Valdenice M. Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A. Palmieri, and Marcos A. Machado -- 3. PCR-based isolation of microsatellite arrays (PIMA) / Heng-Sheng Lin and Song-Bin Chang -- 4. Fast isolation by AFLP of sequences containing repeats / Kaisa Rikalainen -- 5. Microsatellite DNA capture from enriched libraries / Elena G. Gonzalez and Rafael Zardoya -- 6. Next-generation sequencing for high-throughput molecular ecology : a step-by-step protocol for targeted multilocus genotyping by pyrosequencing / Jonathan B. Puritz and Robert J. Toonen -- 7. Optimizing selection of microsatellite loci from 454 pyrosequencing via post-sequencing bioinformatic analyses / Iria Fernandez-Silva and Robert J. Toonen -- 8. Identification of DNA-microsatellite markers for the characterization of somatic embryos in Quercus suber / Arancha Gómez-Garay, Ángeles Bueno, and Beatriz Pintos -- 9. Simple sequence repeats amplification / Kundapura V. Ravishankar and Padmakar Bommisetty -- 10. Microsatellite amplification in plants : optimization procedure of major PCR components / Sana Ghaffari and Nejib Hasnaoui -- 11. Development of a multiplex PCR assay for characterization of embryonic stem cells / Rajarshi Pal, Murali Krishna Mamidi, Anjan Kumar Das, Mahendra Rao and Ramesh Bhonde -- 12. Agarose gel electrophoresis and polyacrylamide gel electrophoresis for visualization of simple sequence repeats / James Anderson, Drew Wright, and Khalid Meksem -- 13. Microsatellite fragment analysis using the ABI PRISM® 377 DNA sequencer / Mark A. Renshaw, Melissa Giresi, and J. Orville Adams -- 14. Robust and inexpensive SSR markers analyses using LI-COR DNA analyzer / Maria del Rosario Herrera and Marc Ghislain -- 15. Use of the MegaBACE for sequencing and genotype analysis / Pamela A. Burger -- 16. Analyzing microsatellites using the QIAxcel system / Deborah A. Dean, Phillip A. Wadl, Denita Hadziabdic, Xinwang and Robert N. Trigiano -- 17. Microsatellite analysis of malaria parasites / Pamela Orjuela-Sanchez, Michelle C. Brandi, and Marcelo U. Ferreira -- 18. Informativeness of microsatellite markers / M. Humberto Reyés-Valdes -- 19. Microsatellite data analysis for population genetics / Kyung Seok Kim and Thomas W. Sappington -- 20. Molecular mapping and breeding with microsatellite markers / David A. Lightfoot and Muhammad J. Iqbal -- 21. Scoring microsatellite loci / Lluvia Flores-Renteria and Andrew Krohn
Control code
825739902
Dimensions
cm.
Extent
xi, 339 pages
Isbn
9781627033886
Isbn Type
(alk. paper)
Lccn
2013934703
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
System control number
(OCoLC)825739902
Label
Microsatellites : methods and protocols, edited by Stella K. Kantartzi
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
1. Microsatellites : evolution and contribution / Panagiotis Madesis, Ioannis Ganopoulos, and Athanasios Tsaftaris -- 2. Screening of genomic libraries / Valdenice M. Novelli, Mariângela Cristofani-Yaly, Marinês Bastianel, Dario A. Palmieri, and Marcos A. Machado -- 3. PCR-based isolation of microsatellite arrays (PIMA) / Heng-Sheng Lin and Song-Bin Chang -- 4. Fast isolation by AFLP of sequences containing repeats / Kaisa Rikalainen -- 5. Microsatellite DNA capture from enriched libraries / Elena G. Gonzalez and Rafael Zardoya -- 6. Next-generation sequencing for high-throughput molecular ecology : a step-by-step protocol for targeted multilocus genotyping by pyrosequencing / Jonathan B. Puritz and Robert J. Toonen -- 7. Optimizing selection of microsatellite loci from 454 pyrosequencing via post-sequencing bioinformatic analyses / Iria Fernandez-Silva and Robert J. Toonen -- 8. Identification of DNA-microsatellite markers for the characterization of somatic embryos in Quercus suber / Arancha Gómez-Garay, Ángeles Bueno, and Beatriz Pintos -- 9. Simple sequence repeats amplification / Kundapura V. Ravishankar and Padmakar Bommisetty -- 10. Microsatellite amplification in plants : optimization procedure of major PCR components / Sana Ghaffari and Nejib Hasnaoui -- 11. Development of a multiplex PCR assay for characterization of embryonic stem cells / Rajarshi Pal, Murali Krishna Mamidi, Anjan Kumar Das, Mahendra Rao and Ramesh Bhonde -- 12. Agarose gel electrophoresis and polyacrylamide gel electrophoresis for visualization of simple sequence repeats / James Anderson, Drew Wright, and Khalid Meksem -- 13. Microsatellite fragment analysis using the ABI PRISM® 377 DNA sequencer / Mark A. Renshaw, Melissa Giresi, and J. Orville Adams -- 14. Robust and inexpensive SSR markers analyses using LI-COR DNA analyzer / Maria del Rosario Herrera and Marc Ghislain -- 15. Use of the MegaBACE for sequencing and genotype analysis / Pamela A. Burger -- 16. Analyzing microsatellites using the QIAxcel system / Deborah A. Dean, Phillip A. Wadl, Denita Hadziabdic, Xinwang and Robert N. Trigiano -- 17. Microsatellite analysis of malaria parasites / Pamela Orjuela-Sanchez, Michelle C. Brandi, and Marcelo U. Ferreira -- 18. Informativeness of microsatellite markers / M. Humberto Reyés-Valdes -- 19. Microsatellite data analysis for population genetics / Kyung Seok Kim and Thomas W. Sappington -- 20. Molecular mapping and breeding with microsatellite markers / David A. Lightfoot and Muhammad J. Iqbal -- 21. Scoring microsatellite loci / Lluvia Flores-Renteria and Andrew Krohn
Control code
825739902
Dimensions
cm.
Extent
xi, 339 pages
Isbn
9781627033886
Isbn Type
(alk. paper)
Lccn
2013934703
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
System control number
(OCoLC)825739902

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