The Resource Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow
Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow
Resource Information
The item Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 2 library branches.
Resource Information
The item Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 2 library branches.
- Summary
- This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
- Language
- eng
- Edition
- 3rd ed.
- Extent
- 1 online resource (xxvi, 200 pages)
- Note
- Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006
- Contents
-
- Lab Session 1 Getting Oriented: Practicing with Micropipettes
- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA
- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation
- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product
- Lab Session 5 DNA Ligation and Transformation of Escherichia coli
- Lab Session 6 Colony Hybridization
- Lab Session 7 Characterization of Recombinant Clones: Part 1
- Lab Session 8 Characterization of Recombinant Clones: Part 2
- Lab Session 9 Characterization of Recombinant Clones: Part 3
- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2
- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1
- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2
- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column
- Lab Session 14 Analysis of Purification Fractions
- Lab Session 15 Total RNA Purification
- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1
- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2
- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1
- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2
- Appendix 1 Equipment
- Appendix 2 Prep List
- Appendix 3 Preparation of Competent E. coli Cells
- Appendix 4 Pre-Lab Questions
- Isbn
- 9780123855459
- Label
- Molecular biology techniques : a classroom laboratory manual
- Title
- Molecular biology techniques
- Title remainder
- a classroom laboratory manual
- Statement of responsibility
- Susan Carson, Heather Miller, D. Scott Witherow
- Subject
-
- DNA, Recombinant
- Electronic books
- Handbooks and manuals
- Handbooks and manuals
- Laboratory manuals
- Laboratory manuals
- Methode
- Methode -- (DE-588c)4038971-6
- Molecular Biology -- methods
- Molecular biology
- Molecular biology -- Laboratory manuals
- Molecular biology -- Technique
- Molecular biology -- Technique -- Handbooks, manuals, etc
- Molekularbiologie
- Molekularbiologie -- (DE-588c)4039983-7
- Protein Engineering
- SCIENCE -- Life Sciences | Molecular Biology
- Language
- eng
- Summary
- This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
- Cataloging source
- E7B
- http://library.link/vocab/creatorName
- Carson, Sue
- Dewey number
- 572.8/078
- Illustrations
- illustrations
- Index
- index present
- LC call number
- QH506
- LC item number
- .C36 2012eb
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- handbooks
- NLM call number
- QU 25
- http://library.link/vocab/relatedWorkOrContributorName
-
- Miller, Heather B
- Witherow, D. Scott
- Series statement
- ClinicalKey
- http://library.link/vocab/subjectName
-
- Molecular biology
- Molecular biology
- DNA, Recombinant
- Molecular Biology
- Protein Engineering
- SCIENCE
- Molecular biology
- Molecular biology
- Methode
- Molekularbiologie
- Methode
- Molekularbiologie
- Label
- Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow
- Note
- Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions
- Control code
- 763161431
- Dimensions
- unknown
- Edition
- 3rd ed.
- Extent
- 1 online resource (xxvi, 200 pages)
- Form of item
- online
- Isbn
- 9780123855459
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- color illustrations.
- http://library.link/vocab/ext/overdrive/overdriveId
- 1104879:10110003
- Specific material designation
- remote
- System control number
- (OCoLC)763161431
- Label
- Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow
- Note
- Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions
- Control code
- 763161431
- Dimensions
- unknown
- Edition
- 3rd ed.
- Extent
- 1 online resource (xxvi, 200 pages)
- Form of item
- online
- Isbn
- 9780123855459
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- color illustrations.
- http://library.link/vocab/ext/overdrive/overdriveId
- 1104879:10110003
- Specific material designation
- remote
- System control number
- (OCoLC)763161431
Subject
- DNA, Recombinant
- Electronic books
- Handbooks and manuals
- Handbooks and manuals
- Laboratory manuals
- Laboratory manuals
- Methode
- Methode -- (DE-588c)4038971-6
- Molecular Biology -- methods
- Molecular biology
- Molecular biology -- Laboratory manuals
- Molecular biology -- Technique
- Molecular biology -- Technique -- Handbooks, manuals, etc
- Molekularbiologie
- Molekularbiologie -- (DE-588c)4039983-7
- Protein Engineering
- SCIENCE -- Life Sciences | Molecular Biology
Genre
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Molecular-biology-techniques--a-classroom/5prIIlTkt7k/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Molecular-biology-techniques--a-classroom/5prIIlTkt7k/">Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
