The Resource Nano- and micro-scale studies of exocytosis, by Won-Chul Shin, (electronic resource)

Nano- and micro-scale studies of exocytosis, by Won-Chul Shin, (electronic resource)

Label
Nano- and micro-scale studies of exocytosis
Title
Nano- and micro-scale studies of exocytosis
Statement of responsibility
by Won-Chul Shin
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Neurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane of cells and release of vesicle contents into the extracellular space in a process called exocytosis. In this thesis, we developed three new nano- and micro- techniques to study exocytosis. 1. We used scanning ion conductance microscopy (SICM) to image changes in the surface membrane of adrenal chromaffin cells after stimulation of exocytosis. Punctate depressions were noted in clusters of two or more. Increases in membrane surface area, consistent with the fusion and collapse of one or more vesicles into the surface membrane, were observed 64% of the cells. 2. We used a microcontact printing method with PDMS stamps by "soft" lithography to pattern microislands of rat hippocampal neurons to form autapses. Neurons on microstamped microislands survived and grew neurites for more than 21 days and resembled microisland cultures formed by the traditional method of spraying collagen on agarose coated substrates. 3. Microfabricated devices were developed to electrochemically measure quantal catecholamine release from an array of individual cells. Here we report patterning of cellsized holes in [tilde operator] 15 m-thick films. These films are placed on transparent indium tin oxide electrodes to insulate the unused part of the electrode whereas the holes in the film both determine the location of the working electrode and serve as pockets for cell trapping. We found that this approach represents a simple and effective way to target cells to electrodes to record amperometric spikes
Cataloging source
MUU
http://library.link/vocab/creatorDate
1973-
http://library.link/vocab/creatorName
Shin, Won-Chul
Degree
Ph.D.
Dissertation year
2007.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Gillis, Kevin D.
http://library.link/vocab/subjectName
  • Exocytosis
  • Conductometric analysis
  • Neurons
  • Paraneurons
Target audience
specialized
Label
Nano- and micro-scale studies of exocytosis, by Won-Chul Shin, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on March 24, 2009)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
316861666
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System control number
(OCoLC)316861666
System details
Mode of access: World Wide Web
Label
Nano- and micro-scale studies of exocytosis, by Won-Chul Shin, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on March 24, 2009)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
316861666
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
c
Specific material designation
remote
System control number
(OCoLC)316861666
System details
Mode of access: World Wide Web

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