Coverart for item
The Resource Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo

Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo

Label
Neutrophil methods and protocols
Title
Neutrophil methods and protocols
Statement of responsibility
edited by Mark T. Quinn, Frank R. DeLeo
Contributor
Subject
Genre
Language
eng
Summary
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells, and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity, and extracellular trap formation; and protocols for investigating neutrophil adhesion, chemotaxis, and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils
Member of
Cataloging source
GW5XE
Dewey number
616.07/9
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP95.8
LC item number
.N48 2014
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • WH 25
NLM item number
ME9616J v.1124 2014
http://library.link/vocab/relatedWorkOrContributorDate
1958-
http://library.link/vocab/relatedWorkOrContributorName
Quinn, Mark Thomas
Series statement
Methods in molecular biology,
Series volume
v. 1124
http://library.link/vocab/subjectName
  • Neutrophils
  • Phagocytes
  • Granulocytes
  • Leukocytes
  • Immune System
  • Cells
  • Myeloid Cells
  • Anatomy
  • Blood Cells
  • Hemic and Immune Systems
  • Blood
  • Neutrophils
  • Neutrophiles
  • Neutrophils
  • Neutrophiler Granulozyt
  • Neutrophiles
Label
Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Subcellular fractionation of human neutrophils and analysis of subcellular markers
  • Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard
  • Rho family and Rap GTPase activation assays
  • Richard T. Jennings and Ulla G. Knaus
  • Measurement of phospholipid metabolism in intact neutrophils
  • Susan Sergeant and Linda C. McPhail
  • Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils
  • Maurice B. Hallett, Maha Al-Jumaa, and Sharon Dewitt
  • Analysis of electrophysiological properties and responses of neutrophils
  • Deri Morgan and Thomas E. DeCoursey
  • The role of neutrophils in the immune system : an overview
  • Assessment of neutrophil apoptosis
  • David A. Dorward ... [and 3 others]
  • Microinjection methods for neutrophils
  • Iraj Laffafian ... [and 3 others]
  • Generation of functionally mature neutrophils from induced pluripotent stem cells
  • Colin L. Sweeney ... [and 6 others]
  • Neutrophil migration through extracellular matrix
  • Richard T. Jennings and Ulla G. Knaus
  • Spinning disk confocal imaging of neutrophil migration in zebrafish
  • Pui-Ying Lam ... [and 4 others]
  • Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn
  • Detection of bidirectional signaling during integrin activation and neutrophil adhesion
  • Stuart M. Altman, Neha Dixit, and Scott I. Simon
  • Immunofluorescence and confocal microscopy of neutrophils
  • Lee-Ann H. Allen
  • Expressin of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils
  • Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein
  • Quantitative assessment of neutrophil phagocytosis using flow cytometry
  • Pontus Nordenfelt
  • Analysis of neutrophil bactericidal activity
  • Heather A. Parker ... [and 4 others]
  • Isolation of human neutrophils from venous blood
  • Induction and quantification of neutrophil extracellular traps
  • Alejandro Sanchez Gonzalez ... [and 3 others]
  • Measurement of respiratory burst products, released or retained, during activation of professional phagocytes
  • Johan Bylund ... [and 4 others]
  • Cell-free NADPH oxidase activation assays : "In vitro veritas"
  • Edgar Pick
  • Assessment of priming of the human neutrophil respiratory burst
  • Margarita Hurtado-Nedelec ... [and 4 others]
  • Affinity pruification and reconstitution of human phagocyte flavocytochrome b for detection of conformational dynamics in the membrane
  • Marcia Riesselman and Algirdas J. Jesaitis
  • William M. Nausef
  • Evaluation of p47phox phosphorylation in human neutrophils using phospho-specicfic antibodies
  • Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna
  • Genome-scale transcript analyses with human neutrophils
  • Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo
  • Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils
  • Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni
  • High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis
  • Flavia S. Lakschevitz and Michael Glogauer
  • Detection of intact transciption factors in human neutrophils
  • Patrick P. McDonald and Richard D. Ye
  • Neutrophil isolation from nonhuman species
  • Disorders of neutrophil function : an overview
  • Mary C. Dinauer
  • Diagnostic assays for chronic granulomatous disease and other neutrophil disorders
  • Houda Zghal Elloumi and Steven M. Holland
  • Diagnostic assays for myeloperoxidase and myeloperoxidase deficiency
  • William M. Nauseef
  • Daniel W. Siemsen ... [and 7 others]
  • Collection of in vivo transmigrated neutrophils from human skin
  • Karin Christenson ... [and 6 others]
Control code
870649932
Dimensions
unknown
Edition
Second edition.
Extent
1 online resource (xvi, 551 pages)
File format
unknown
Form of item
online
Isbn
9781627038454
Lccn
2014930433
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-845-4
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)870649932
Label
Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Subcellular fractionation of human neutrophils and analysis of subcellular markers
  • Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard
  • Rho family and Rap GTPase activation assays
  • Richard T. Jennings and Ulla G. Knaus
  • Measurement of phospholipid metabolism in intact neutrophils
  • Susan Sergeant and Linda C. McPhail
  • Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils
  • Maurice B. Hallett, Maha Al-Jumaa, and Sharon Dewitt
  • Analysis of electrophysiological properties and responses of neutrophils
  • Deri Morgan and Thomas E. DeCoursey
  • The role of neutrophils in the immune system : an overview
  • Assessment of neutrophil apoptosis
  • David A. Dorward ... [and 3 others]
  • Microinjection methods for neutrophils
  • Iraj Laffafian ... [and 3 others]
  • Generation of functionally mature neutrophils from induced pluripotent stem cells
  • Colin L. Sweeney ... [and 6 others]
  • Neutrophil migration through extracellular matrix
  • Richard T. Jennings and Ulla G. Knaus
  • Spinning disk confocal imaging of neutrophil migration in zebrafish
  • Pui-Ying Lam ... [and 4 others]
  • Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn
  • Detection of bidirectional signaling during integrin activation and neutrophil adhesion
  • Stuart M. Altman, Neha Dixit, and Scott I. Simon
  • Immunofluorescence and confocal microscopy of neutrophils
  • Lee-Ann H. Allen
  • Expressin of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils
  • Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein
  • Quantitative assessment of neutrophil phagocytosis using flow cytometry
  • Pontus Nordenfelt
  • Analysis of neutrophil bactericidal activity
  • Heather A. Parker ... [and 4 others]
  • Isolation of human neutrophils from venous blood
  • Induction and quantification of neutrophil extracellular traps
  • Alejandro Sanchez Gonzalez ... [and 3 others]
  • Measurement of respiratory burst products, released or retained, during activation of professional phagocytes
  • Johan Bylund ... [and 4 others]
  • Cell-free NADPH oxidase activation assays : "In vitro veritas"
  • Edgar Pick
  • Assessment of priming of the human neutrophil respiratory burst
  • Margarita Hurtado-Nedelec ... [and 4 others]
  • Affinity pruification and reconstitution of human phagocyte flavocytochrome b for detection of conformational dynamics in the membrane
  • Marcia Riesselman and Algirdas J. Jesaitis
  • William M. Nausef
  • Evaluation of p47phox phosphorylation in human neutrophils using phospho-specicfic antibodies
  • Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna
  • Genome-scale transcript analyses with human neutrophils
  • Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo
  • Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils
  • Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni
  • High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis
  • Flavia S. Lakschevitz and Michael Glogauer
  • Detection of intact transciption factors in human neutrophils
  • Patrick P. McDonald and Richard D. Ye
  • Neutrophil isolation from nonhuman species
  • Disorders of neutrophil function : an overview
  • Mary C. Dinauer
  • Diagnostic assays for chronic granulomatous disease and other neutrophil disorders
  • Houda Zghal Elloumi and Steven M. Holland
  • Diagnostic assays for myeloperoxidase and myeloperoxidase deficiency
  • William M. Nauseef
  • Daniel W. Siemsen ... [and 7 others]
  • Collection of in vivo transmigrated neutrophils from human skin
  • Karin Christenson ... [and 6 others]
Control code
870649932
Dimensions
unknown
Edition
Second edition.
Extent
1 online resource (xvi, 551 pages)
File format
unknown
Form of item
online
Isbn
9781627038454
Lccn
2014930433
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-845-4
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)870649932

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      38.944491 -92.326012
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