Coverart for item
The Resource Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch

Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch

Label
Neutrophil methods and protocols
Title
Neutrophil methods and protocols
Statement of responsibility
edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch
Contributor
Subject
Genre
Language
eng
Summary
Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils
Member of
Cataloging source
COO
Dewey number
616.07/9
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP95.8
LC item number
.N48 2007
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • WH 25
NLM item number
  • ME9616J v.412 2007
  • N497 2007
http://library.link/vocab/relatedWorkOrContributorDate
1958-
http://library.link/vocab/relatedWorkOrContributorName
  • Quinn, Mark Thomas
  • Deleo, Frank
  • Bokoch, Gary M
Series statement
Methods in molecular biology
Series volume
138 [i.e. 412]
http://library.link/vocab/subjectName
  • Neutrophils
  • Neutrophils
  • Neutrophiles
  • MEDICAL
  • Neutrophils
  • Neutrophils
  • Neutrophiler Granulozyt
  • Neutrophiles
Label
Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
The role of neutrophils in the immune system: an overview / H.L. Malech -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / L. Udby and N. Borregaard -- Rac and Rap GTPase activation assays / U.G. Knaus, A. Bamberg and G.M. Bokoch -- Measurement of phospholipid metabolism in intact neutrophils / S. Sergeant and L.C. McPhail -- Analysis of protein phosphorylation in human neutrophils / J. El-Benna and P.M.-C. Dang -- Mitogen-activated protein kinase assays / M. Torres -- Transduction of proteins into intact neutrophils / T. Zhao and G.M. Bokoch -- Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils / E.J. Hillson, S. Dewitt and M.B. Hallett -- Analysis of electrophysiological properties and responses of neutrophils / D. Morgan and T.E. DeCoursey -- Analysis of neutrophil apoptosis / E.L. Taylor [and others] -- Optical and fluorescence detection of neutrophil integrin activation / U.Y. Schaff [and others] -- Analysis of neutrophil polarization and chemotaxis / M.A. Lokuta, P.A. Nuzzi and A. Huttenlocher -- Quantifying and localizing actin-free barbed ends in neutrophils / M. Glogauer -- Measurement of neutrophil adhesion under conditions mimicking blood flow / M.A. Jutila [and others] -- Model systems to investigate neutrophil adhesion and chemotaxis / N.A. Louis, E. Campbell and S.P. Colgan -- Immunofluorescence and confocal microscopy of neutrophils / L.-A.H. Allen -- Assessment of phagosome formation and maturation by fluorescence microscopy / B.E. Steinberg and S. Grinstein -- Analysis of neutrophil membrane traffic during phagocytosis / P. Loennbro, P. Nordenfelt and H. Tapper -- Analysis of neutrophil bactericidal activity / J.N. Green, C.C. Winterbourn and M.B. Hampton -- A skin chamber technique as a human model for studies of aseptic inflammatory reactions / P. Follin and C. Dahlgren -- Measurement of respiratory burst products generated by professional phagocytes / C. Dahlgren, A. Karlsson and J. Bylund -- The K-562 cell model for analysis of neutrophil NADPH oxidase function / T.L. Leto [and others] -- Cell-free assays: the reductionist approach to the study of NADPH oxidase assembly, or "all you wanted to know about cell-free assays but did not dare to ask" / S. Molshanski-Mor [and others] -- Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics / R.M. Taylor and A.J. Jesaitis -- Genome-scale transcript analyses in human neutrophils / S.D. Kobayashi, D.E. Sturdevant and F.R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils by reverse transcription real-time PCR / N. Tamassia, M.A. Cassatella and F. Bazzoni -- Detection of intact transcription factors in human neutrophils / P.P. McDonald and R.D. Ye -- Disorders of neutrophil function: an overview / M.C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / H.Z. Elloumi and S.M. Holland -- Diagnostic assays for myeloperoxidase deficiency / W.M. Nauseef
Control code
241307851
Dimensions
unknown
Extent
1 online resource (xx, 537 pages)
Form of item
online
Isbn
9781280972034
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
  • 10.1007/978-1-59745-467-4.
  • 9786610972036
Other physical details
illustrations (some color)
http://library.link/vocab/ext/overdrive/overdriveId
97203
Specific material designation
remote
System control number
(OCoLC)241307851
Label
Neutrophil methods and protocols, edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
The role of neutrophils in the immune system: an overview / H.L. Malech -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / L. Udby and N. Borregaard -- Rac and Rap GTPase activation assays / U.G. Knaus, A. Bamberg and G.M. Bokoch -- Measurement of phospholipid metabolism in intact neutrophils / S. Sergeant and L.C. McPhail -- Analysis of protein phosphorylation in human neutrophils / J. El-Benna and P.M.-C. Dang -- Mitogen-activated protein kinase assays / M. Torres -- Transduction of proteins into intact neutrophils / T. Zhao and G.M. Bokoch -- Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils / E.J. Hillson, S. Dewitt and M.B. Hallett -- Analysis of electrophysiological properties and responses of neutrophils / D. Morgan and T.E. DeCoursey -- Analysis of neutrophil apoptosis / E.L. Taylor [and others] -- Optical and fluorescence detection of neutrophil integrin activation / U.Y. Schaff [and others] -- Analysis of neutrophil polarization and chemotaxis / M.A. Lokuta, P.A. Nuzzi and A. Huttenlocher -- Quantifying and localizing actin-free barbed ends in neutrophils / M. Glogauer -- Measurement of neutrophil adhesion under conditions mimicking blood flow / M.A. Jutila [and others] -- Model systems to investigate neutrophil adhesion and chemotaxis / N.A. Louis, E. Campbell and S.P. Colgan -- Immunofluorescence and confocal microscopy of neutrophils / L.-A.H. Allen -- Assessment of phagosome formation and maturation by fluorescence microscopy / B.E. Steinberg and S. Grinstein -- Analysis of neutrophil membrane traffic during phagocytosis / P. Loennbro, P. Nordenfelt and H. Tapper -- Analysis of neutrophil bactericidal activity / J.N. Green, C.C. Winterbourn and M.B. Hampton -- A skin chamber technique as a human model for studies of aseptic inflammatory reactions / P. Follin and C. Dahlgren -- Measurement of respiratory burst products generated by professional phagocytes / C. Dahlgren, A. Karlsson and J. Bylund -- The K-562 cell model for analysis of neutrophil NADPH oxidase function / T.L. Leto [and others] -- Cell-free assays: the reductionist approach to the study of NADPH oxidase assembly, or "all you wanted to know about cell-free assays but did not dare to ask" / S. Molshanski-Mor [and others] -- Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics / R.M. Taylor and A.J. Jesaitis -- Genome-scale transcript analyses in human neutrophils / S.D. Kobayashi, D.E. Sturdevant and F.R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils by reverse transcription real-time PCR / N. Tamassia, M.A. Cassatella and F. Bazzoni -- Detection of intact transcription factors in human neutrophils / P.P. McDonald and R.D. Ye -- Disorders of neutrophil function: an overview / M.C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / H.Z. Elloumi and S.M. Holland -- Diagnostic assays for myeloperoxidase deficiency / W.M. Nauseef
Control code
241307851
Dimensions
unknown
Extent
1 online resource (xx, 537 pages)
Form of item
online
Isbn
9781280972034
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
  • 10.1007/978-1-59745-467-4.
  • 9786610972036
Other physical details
illustrations (some color)
http://library.link/vocab/ext/overdrive/overdriveId
97203
Specific material designation
remote
System control number
(OCoLC)241307851

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