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The Resource Nuclear pore complexes and nucleocytoplasmic transport - Methods, edited by Valérie Doye

Nuclear pore complexes and nucleocytoplasmic transport - Methods, edited by Valérie Doye

Label
Nuclear pore complexes and nucleocytoplasmic transport - Methods
Title
Nuclear pore complexes and nucleocytoplasmic transport - Methods
Statement of responsibility
edited by Valérie Doye
Contributor
Editor
Subject
Language
eng
Summary
Volume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems (including mammalian cells, Xenopus, C. elegans, yeast). The volume enables investigators to analyze nuclear pore complex structure, assembly, and dynamics; to evaluate protein and RNA trafficking through the nuclear envelope; and to design in vivo or in vitro assays appropriate to their research needs. Beyond the study of nuclear pores and transport as such, these protocols will also
Member of
Cataloging source
EBLCP
Dewey number
574.87/32
Illustrations
illustrations
Index
no index present
LC call number
QH595
LC item number
.N384 2014eb
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Doye, Valérie
Series statement
Methods in cell biology
Series volume
volume 122
http://library.link/vocab/subjectName
  • Cell nuclei
  • Nuclear membranes
  • Cytoplasm
  • Biological transport
  • Active Transport, Cell Nucleus
  • Cell Nucleus
  • Nuclear Pore
  • Cytological Techniques
  • Cell Biology
  • Cell nuclei
  • Science
  • SCIENCE
  • Biological transport
  • Cell nuclei
  • Cytoplasm
  • Nuclear membranes
Label
Nuclear pore complexes and nucleocytoplasmic transport - Methods, edited by Valérie Doye
Instantiates
Publication
Copyright
Note
3.1.2. Procedure
Antecedent source
unknown
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Contents
  • Front Cover; Nuclear Pore Complexes and Nucleocytoplasmic Transport -- Methods; Copyright; Contents; Contributors; Preface; Chapter 1: Fifty Years of Nuclear Pores and Nucleocytoplasmic Transport Studies: Multiple Tools Revealing Complex Rules; Introduction; 1.1. The NPCs: A Modular Macromolecular Assembly; 1.1.1. Toward a refined view of the NPC structure; 1.1.2. Nucleoporins: The building blocks of NPCs; 1.1.3. Integrating the Nups into the 3D architecture of the NPC; 1.1.3.1. Nup localization; 1.1.3.2. Interactions among Nups; 1.1.3.3. Nup stoichiometry
  • 1.1.3.4. Toward a detailed map of NPCs1.1.4. Nups are composed of a limited set of structural domains; 1.2. Nucleocytoplasmic Trafficking: The Rules of the Road; 1.2.1. Investigating nucleocytoplasmic transport; 1.2.1.1. Historical overview; 1.2.1.2. Expanding the toolbox; 1.2.2. The signals for nucleocytoplasmic exchanges; 1.2.3. A family of protein transport receptors: The karyopherins; 1.2.4. The Ran GTPase: A key to transport directionality; 1.2.5. The case of INM targeting; 1.2.6. Distinct pathways contribute to RNA export
  • 1.2.7. Translocation across the NPCs: A dual function for FG-Nups as barrier and gate1.2.8. Noncanonical transport pathways through the NE; 1.3. The Nuclear Transport Machinery: A Dynamic and Versatile Device; 1.3.1. NPC biogenesis throughout the cell cycle; 1.3.1.1. NPC disassembly; 1.3.1.2. Post-mitotic NPC assembly; 1.3.1.3. De novo NPC assembly; 1.3.2. Multiple functions of the nuclear transport machinery during the cell cycle; 1.3.3. NPCs, nuclear organization, and gene expression; 1.3.4. NPCs and genetic stability; Concluding Remarks; Acknowledgments; References
  • Chapter 2: Imaging Metazoan Nuclear Pore Complexes by Field Emission Scanning Electron MicroscopyIntroduction; Prehistoric landmarks (before EM): From cells to the nuclear envelope; The EM era: From the nuclear envelope to nuclear pore complexes; High resolution SEM: Direct surface imaging of NPCs; 2.1. Rationale; 2.1.1. Purpose; 2.1.2. An overview of sample preparation; 2.1.2.1. Fixation; 2.1.2.2. Dehydration and critical point drying; 2.1.2.3. Sputter coating; 2.2. Materials; 2.2.1. Equipment; 2.2.2. Materials; 2.2.3. Reagents; 2.2.4. Buffers; 2.3. Anchored Nuclei
  • 2.3.1. Coating silicon chips2.3.2. Chromatin decondensation and attachment; 2.3.3. In vitro assembly reaction; 2.4. Mammalian Cell Nuclei; 2.5. Immunogold Labeling; 2.6. Sample Preparation for FESEM; 2.6.1. Fixation, dehydration, and CPD; 2.6.2. Sputter coating and FESEM analysis; Acknowledgments; References; Chapter 3: Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM; Introduction; 3.1. Conventional TEM; 3.1.1. Materials; 3.1.1.1. Equipment; 3.1.1.2. Spheroplast preparation; 3.1.1.3. Fixation, dehydration, contrasting, and embedding; 3.1.1.4. Sectioning and poststaining
Control code
880827118
Dimensions
unknown
Edition
First edition.
Extent
1 online resource (xx, 508 pages)
File format
unknown
Form of item
online
Isbn
9780124171787
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Other physical details
color illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)880827118
Label
Nuclear pore complexes and nucleocytoplasmic transport - Methods, edited by Valérie Doye
Publication
Copyright
Note
3.1.2. Procedure
Antecedent source
unknown
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Contents
  • Front Cover; Nuclear Pore Complexes and Nucleocytoplasmic Transport -- Methods; Copyright; Contents; Contributors; Preface; Chapter 1: Fifty Years of Nuclear Pores and Nucleocytoplasmic Transport Studies: Multiple Tools Revealing Complex Rules; Introduction; 1.1. The NPCs: A Modular Macromolecular Assembly; 1.1.1. Toward a refined view of the NPC structure; 1.1.2. Nucleoporins: The building blocks of NPCs; 1.1.3. Integrating the Nups into the 3D architecture of the NPC; 1.1.3.1. Nup localization; 1.1.3.2. Interactions among Nups; 1.1.3.3. Nup stoichiometry
  • 1.1.3.4. Toward a detailed map of NPCs1.1.4. Nups are composed of a limited set of structural domains; 1.2. Nucleocytoplasmic Trafficking: The Rules of the Road; 1.2.1. Investigating nucleocytoplasmic transport; 1.2.1.1. Historical overview; 1.2.1.2. Expanding the toolbox; 1.2.2. The signals for nucleocytoplasmic exchanges; 1.2.3. A family of protein transport receptors: The karyopherins; 1.2.4. The Ran GTPase: A key to transport directionality; 1.2.5. The case of INM targeting; 1.2.6. Distinct pathways contribute to RNA export
  • 1.2.7. Translocation across the NPCs: A dual function for FG-Nups as barrier and gate1.2.8. Noncanonical transport pathways through the NE; 1.3. The Nuclear Transport Machinery: A Dynamic and Versatile Device; 1.3.1. NPC biogenesis throughout the cell cycle; 1.3.1.1. NPC disassembly; 1.3.1.2. Post-mitotic NPC assembly; 1.3.1.3. De novo NPC assembly; 1.3.2. Multiple functions of the nuclear transport machinery during the cell cycle; 1.3.3. NPCs, nuclear organization, and gene expression; 1.3.4. NPCs and genetic stability; Concluding Remarks; Acknowledgments; References
  • Chapter 2: Imaging Metazoan Nuclear Pore Complexes by Field Emission Scanning Electron MicroscopyIntroduction; Prehistoric landmarks (before EM): From cells to the nuclear envelope; The EM era: From the nuclear envelope to nuclear pore complexes; High resolution SEM: Direct surface imaging of NPCs; 2.1. Rationale; 2.1.1. Purpose; 2.1.2. An overview of sample preparation; 2.1.2.1. Fixation; 2.1.2.2. Dehydration and critical point drying; 2.1.2.3. Sputter coating; 2.2. Materials; 2.2.1. Equipment; 2.2.2. Materials; 2.2.3. Reagents; 2.2.4. Buffers; 2.3. Anchored Nuclei
  • 2.3.1. Coating silicon chips2.3.2. Chromatin decondensation and attachment; 2.3.3. In vitro assembly reaction; 2.4. Mammalian Cell Nuclei; 2.5. Immunogold Labeling; 2.6. Sample Preparation for FESEM; 2.6.1. Fixation, dehydration, and CPD; 2.6.2. Sputter coating and FESEM analysis; Acknowledgments; References; Chapter 3: Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM; Introduction; 3.1. Conventional TEM; 3.1.1. Materials; 3.1.1.1. Equipment; 3.1.1.2. Spheroplast preparation; 3.1.1.3. Fixation, dehydration, contrasting, and embedding; 3.1.1.4. Sectioning and poststaining
Control code
880827118
Dimensions
unknown
Edition
First edition.
Extent
1 online resource (xx, 508 pages)
File format
unknown
Form of item
online
Isbn
9780124171787
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Other physical details
color illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)880827118

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