Coverart for item
The Resource Oligonucleotide synthesis : methods and applications, edited by Piet Herdewijn

Oligonucleotide synthesis : methods and applications, edited by Piet Herdewijn

Label
Oligonucleotide synthesis : methods and applications
Title
Oligonucleotide synthesis
Title remainder
methods and applications
Statement of responsibility
edited by Piet Herdewijn
Contributor
Subject
Genre
Language
eng
Summary
Nucleic acids chemistry has progressed rapidly in the last 10 years with the introduction of many new techniques and the development of many novel therapeutic entities. In Oligonucleotide Synthesis: Methods and Applications, laboratory experts describe in step-by-step detail the powerful new techniques they have developed for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are the synthesis of phosphorothioates via new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, triple helix formation, and a novel two-step process that yields a high purity DNA that is less costly. Novel applications include the use of molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules to DNA, nucleic acid libraries, optimization of RNA synthesis for siRNA applications, and the use of small interference RNA (siRNA) as an inhibitor of gene expression. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Oligonucleotide Synthesis: Methods and Applications offers today's investigators not only insight into important new biotechnologies, but also a full range of the detailed protocols needed to work successfully in DNA synthesis and its applications today
Member of
Cataloging source
COO
Dewey number
572.8/5
Illustrations
illustrations
Index
index present
LC call number
QP625.O47
LC item number
O54 2005
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 288
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.288 2004
  • O465 2004
http://library.link/vocab/relatedWorkOrContributorName
Herdewijn, Piet
Series statement
Methods in molecular biology
Series volume
288
http://library.link/vocab/subjectName
  • Oligonucleotides
  • Oligonucleotides
  • Oligonucleotides
  • SCIENCE
  • Oligonucleotides
Label
Oligonucleotide synthesis : methods and applications, edited by Piet Herdewijn
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger [and others] -- RNA synthesis using 2'-o-(tert-butyldimethylsilyl) protection / Brian S. Sproat -- RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / S. Hartsel [and others] -- DTD; new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis -- Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi -- Assay for evaluating ribonuclease H-mediated degradation of RNA/antisense oligonucleotide duplexes / Annie Galarneau [and others] -- Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg -- Solid-phase chemistry of circular oligonucleotides / Enrique Pedroso [and others] -- LNA synthesis / Henrik M. Pfundheller [and others] -- Synthesis of DNA mimics representing HypNa-pPNA heterooligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva -- Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela [and others] -- Introduction of hypermodified nucleotides in rna / Darell Davis and Ashok C. Bajji -- Chemical methods for peptide/oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait -- Biotin labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho [and others] -- Universal labeling chemistry for nucleic acid detection on DNA-chips -- Ali Laayoun, Eloy [and others] -- Post-synthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun -- Fluorescence based online detection as an analytical tool in RNA electrophoresis / Ute Scheffer and michael göbel -- Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras -- High thoughput production of optimized primers (fimers) for whole genome direct sequencing / Nikolai Polushin [and others] -- Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel -- DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly, Jérome Kluza, Christopher Martin / Thomas Ellis, and Michael J. Waring -- Gene targeting using PNA (peptide nucleic acid) / Peter E. Nielsen -- Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau -- In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke -- In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse [and others] -- Short double-stranded RNA (sirna) as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy
Control code
61354890
Dimensions
unknown
Extent
1 online resource (xvi, 435 pages)
Form of item
online
Isbn
9781592598236
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
http://library.link/vocab/ext/overdrive/overdriveId
978-1-58829-233-9
Specific material designation
remote
System control number
(OCoLC)61354890
Label
Oligonucleotide synthesis : methods and applications, edited by Piet Herdewijn
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger [and others] -- RNA synthesis using 2'-o-(tert-butyldimethylsilyl) protection / Brian S. Sproat -- RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / S. Hartsel [and others] -- DTD; new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis -- Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi -- Assay for evaluating ribonuclease H-mediated degradation of RNA/antisense oligonucleotide duplexes / Annie Galarneau [and others] -- Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg -- Solid-phase chemistry of circular oligonucleotides / Enrique Pedroso [and others] -- LNA synthesis / Henrik M. Pfundheller [and others] -- Synthesis of DNA mimics representing HypNa-pPNA heterooligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva -- Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela [and others] -- Introduction of hypermodified nucleotides in rna / Darell Davis and Ashok C. Bajji -- Chemical methods for peptide/oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait -- Biotin labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho [and others] -- Universal labeling chemistry for nucleic acid detection on DNA-chips -- Ali Laayoun, Eloy [and others] -- Post-synthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun -- Fluorescence based online detection as an analytical tool in RNA electrophoresis / Ute Scheffer and michael göbel -- Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras -- High thoughput production of optimized primers (fimers) for whole genome direct sequencing / Nikolai Polushin [and others] -- Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel -- DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly, Jérome Kluza, Christopher Martin / Thomas Ellis, and Michael J. Waring -- Gene targeting using PNA (peptide nucleic acid) / Peter E. Nielsen -- Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau -- In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke -- In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse [and others] -- Short double-stranded RNA (sirna) as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy
Control code
61354890
Dimensions
unknown
Extent
1 online resource (xvi, 435 pages)
Form of item
online
Isbn
9781592598236
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
http://library.link/vocab/ext/overdrive/overdriveId
978-1-58829-233-9
Specific material designation
remote
System control number
(OCoLC)61354890

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