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The Resource Optical glucose nanobiosensor encapsulated in erythrocytes, by Majed El-Dweik, (electronic resource)

Optical glucose nanobiosensor encapsulated in erythrocytes, by Majed El-Dweik, (electronic resource)

Creator
Contributor
Summary
An implantable glucose biosensor encapsulated in erythrocytes, Red Blood Cells (RBC), will become a method for continuously measuring blood glucose concentration in diabetics. In 2005, the American Diabetes Association (ADA) reported that 20.8 million people have diabetes, making it the fifth leading cause of death by disease in the USA. This paper focuses on the preparation phase of the glucose sensor. Glucose Binding Protein (GBP) from E. coli was labeled with two fluorophores, Alexa Fluor 680 (AF680), and Alexa Fluor 750 (AF750). This sensor based on Fluorescence Resonance Energy Transfer (FRET). FRET is a distance sensitive technique between the above fluorophores. The initial energy transfer between AF680 and AF750 labeled on the GBP before glucose additions was determined. After glucose additions, the labeled GBP went through conformational change which caused distance between the labeled sites. This change in distance caused a change in the energy transfer. The labeled GBP became the glucose nanobiosensor. The labeled GBP nanobiosensors were encapsulated in erythrocytes, red blood cells (RBCs), by using the Hypo-Osmotic dialysis technique. The encapsulated RBCs responded well to different glucose concentrations ranging form 0-33.16mM. This range covers the normal blood glucose concentration, 4 - 9mM
Language
eng
Work
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on March 23, 2009)
  • Vita
Instance
Label
Optical glucose nanobiosensor encapsulated in erythrocytes
Title
Optical glucose nanobiosensor encapsulated in erythrocytes
Statement of responsibility
by Majed El-Dweik
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
An implantable glucose biosensor encapsulated in erythrocytes, Red Blood Cells (RBC), will become a method for continuously measuring blood glucose concentration in diabetics. In 2005, the American Diabetes Association (ADA) reported that 20.8 million people have diabetes, making it the fifth leading cause of death by disease in the USA. This paper focuses on the preparation phase of the glucose sensor. Glucose Binding Protein (GBP) from E. coli was labeled with two fluorophores, Alexa Fluor 680 (AF680), and Alexa Fluor 750 (AF750). This sensor based on Fluorescence Resonance Energy Transfer (FRET). FRET is a distance sensitive technique between the above fluorophores. The initial energy transfer between AF680 and AF750 labeled on the GBP before glucose additions was determined. After glucose additions, the labeled GBP went through conformational change which caused distance between the labeled sites. This change in distance caused a change in the energy transfer. The labeled GBP became the glucose nanobiosensor. The labeled GBP nanobiosensors were encapsulated in erythrocytes, red blood cells (RBCs), by using the Hypo-Osmotic dialysis technique. The encapsulated RBCs responded well to different glucose concentrations ranging form 0-33.16mM. This range covers the normal blood glucose concentration, 4 - 9mM
Cataloging source
MUU
http://library.link/vocab/creatorDate
1966-
http://library.link/vocab/creatorName
El-Dweik, Majed
Degree
Ph.D.
Dissertation year
2007.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Grant, Sheila Ann
http://library.link/vocab/subjectName
  • Diabetes
  • Glucose
  • Biosensors
  • Erythrocytes
Target audience
specialized
Label
Optical glucose nanobiosensor encapsulated in erythrocytes, by Majed El-Dweik, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on March 23, 2009)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
316804933
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)316804933
System details
Mode of access: World Wide Web
Label
Optical glucose nanobiosensor encapsulated in erythrocytes, by Majed El-Dweik, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on March 23, 2009)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
316804933
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)316804933
System details
Mode of access: World Wide Web

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