Coverart for item
The Resource PRINS and in situ PCR protocols, edited by John R. Gosden

PRINS and in situ PCR protocols, edited by John R. Gosden

Label
PRINS and in situ PCR protocols
Title
PRINS and in situ PCR protocols
Statement of responsibility
edited by John R. Gosden
Contributor
Subject
Genre
Language
eng
Summary
Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells
Member of
Action
digitized
Cataloging source
YUS
Dewey number
574.87/3282
Illustrations
illustrations
Index
index present
LC call number
QH452.8
LC item number
.P75 1997
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 450.8
NLM item number
  • ME9616J v.71 1997
  • P957 1997
http://library.link/vocab/relatedWorkOrContributorName
Gosden, John R
Series statement
Methods in molecular biology
Series volume
71
http://library.link/vocab/subjectName
  • In situ hybridization
  • Polymerase chain reaction
  • DNA Primers
  • Polymerase Chain Reaction
  • Sequence Analysis
  • Situ Hybridization
  • Chromosome Mapping
  • In situ hybridization
  • Polymerase chain reaction
  • Polymerase kettingreactie
  • Wetenschappelijke technieken
Label
PRINS and in situ PCR protocols, edited by John R. Gosden
Instantiates
Publication
Note
Source of title; title from title page
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Oligonucleotide PRINS DNA synthesis / John R. Gosden, Diane Lawson -- Chromosome-specific PRINS / Jean-Paul Charlieu, Franck Pellestor -- Bright-field microscopic detection of oligonucleotide PRINS-labeled DNA in chromosome preparations / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Analysis of sperm aneuploidy by PRINS / Franck Pellestor, Jean-Paul Charlieu -- PRINS DNA synthesis on frozen tissue sections / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Multiple sequential oligonucleotide primed in situ DNA syntheses (MULTI-PRINS) / John R. Gosden, Diane Lawson -- Oligonucleotide PRINS DNA synthesis on extended chromatin preparations / Yoshiro Shibasaki, John R. Gosden -- Combined immunocytochemistry and PRINS DNA synthesis for simultaneous detection of phenotypic and genomic parameters in cells / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Chromosomal PRINS DNA labeling combined with indirect immunocytochemistry / Arthur R. Mitchell -- Direct in situ single-copy (DISC)-PCR / Deryl L. Troyer, Hongyan Xie, Jie Hu -- In situ PCR to cells and to wax sections: an alternative to in situ hybridization for the detection of low copies of human papillomavirus and cytomegalovirus DNA / Christiane Mougin, Jean Marc Didier, Dominique Bettinger, Laurence Madoz, Sylviane Coumes-Marquet, Michel Lab -- Reverse transcriptase PCR in situ on cryopreserved tissue sections / Fred J. Hendler, Alice Shum-Siu, Briana W. Heniford, Mohammed El Ahmed, V.J. Patel -- Flow cytometry analysis of an in situ PCR for the detection of human immunodeficiency virus type-1 (HIV-1) proviral DNA / David E. Gibellini, Maria Carla Re, Giuliano Furlini, Michele Placa -- In situ PCR amplification of intracellular mRNA / Raymond H. Chen, Susan V. Fuggle -- Localized in situ amplification (LISA) : PCR amplification of nucleic acid sequences in tissue sections / Gregory J. Tsongalis -- In situ PCR : an overview / Aldan A. Long, Paul Komminoth
Control code
55522400
Dimensions
unknown
Extent
1 online resource (xiii, 165 pages)
File format
unknown
Form of item
online
Isbn
9781592595570
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896033953
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)55522400
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
PRINS and in situ PCR protocols, edited by John R. Gosden
Publication
Note
Source of title; title from title page
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Oligonucleotide PRINS DNA synthesis / John R. Gosden, Diane Lawson -- Chromosome-specific PRINS / Jean-Paul Charlieu, Franck Pellestor -- Bright-field microscopic detection of oligonucleotide PRINS-labeled DNA in chromosome preparations / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Analysis of sperm aneuploidy by PRINS / Franck Pellestor, Jean-Paul Charlieu -- PRINS DNA synthesis on frozen tissue sections / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Multiple sequential oligonucleotide primed in situ DNA syntheses (MULTI-PRINS) / John R. Gosden, Diane Lawson -- Oligonucleotide PRINS DNA synthesis on extended chromatin preparations / Yoshiro Shibasaki, John R. Gosden -- Combined immunocytochemistry and PRINS DNA synthesis for simultaneous detection of phenotypic and genomic parameters in cells / Ernst J. Speel, Diane Lawson, Frans C. Ramaekers, John R. Gosden, Anton H. Hopman -- Chromosomal PRINS DNA labeling combined with indirect immunocytochemistry / Arthur R. Mitchell -- Direct in situ single-copy (DISC)-PCR / Deryl L. Troyer, Hongyan Xie, Jie Hu -- In situ PCR to cells and to wax sections: an alternative to in situ hybridization for the detection of low copies of human papillomavirus and cytomegalovirus DNA / Christiane Mougin, Jean Marc Didier, Dominique Bettinger, Laurence Madoz, Sylviane Coumes-Marquet, Michel Lab -- Reverse transcriptase PCR in situ on cryopreserved tissue sections / Fred J. Hendler, Alice Shum-Siu, Briana W. Heniford, Mohammed El Ahmed, V.J. Patel -- Flow cytometry analysis of an in situ PCR for the detection of human immunodeficiency virus type-1 (HIV-1) proviral DNA / David E. Gibellini, Maria Carla Re, Giuliano Furlini, Michele Placa -- In situ PCR amplification of intracellular mRNA / Raymond H. Chen, Susan V. Fuggle -- Localized in situ amplification (LISA) : PCR amplification of nucleic acid sequences in tissue sections / Gregory J. Tsongalis -- In situ PCR : an overview / Aldan A. Long, Paul Komminoth
Control code
55522400
Dimensions
unknown
Extent
1 online resource (xiii, 165 pages)
File format
unknown
Form of item
online
Isbn
9781592595570
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896033953
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)55522400
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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