The Resource Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri
Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri
Resource Information
The item Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods, and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature
- Language
- eng
- Extent
- 1 online resource (x, 292 pages)
- Contents
-
- Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus
- Pauline T. Lieu [and others]
- Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions
- Andrew Fontes [and others]
- Feeder-free substrates for pluripotent stem cell culture
- Alexandria Sams and Mark J. Powers
- Methods for culturing human embryonic stem cells on feeders
- Jasmeet Kaur and Mary Lynn Tilkins
- Methods for culturing human embryonic stem cells in a xeno-free system
- Jasmeet Kaur [and others]
- Sources and derivation of human embryonic stem cells
- Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage
- Dennis Van Hoof and Muluye E. Liku
- Directed differentiation of pluripotent stem cells to functional hepatocytes
- Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
- Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes
- Paul W. Burridge and Elias T. Zambidis
- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors
- Kejin Hu and Igor Slukvin
- Cellular characterization of human pluripotent stem cells
- Rene H. Quintanilla Jr.
- Michal Amit
- TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells
- Sunali N. Patel [and others]
- Epigenome analysis of pluripotent stem cells
- Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
- Single cell gene expression analysis of pluripotent stem cells
- Ronald V. Abruzzese and Richard A. Fekete
- Profiling stem cells using quantitative PCR protein assays
- David Ruff and Pauline T. Lieu
- Labeled stem cells as disease models and in drug discovery
- Catharina Ellerstrom, Raimund Strehl, and Johan Hyllner
- New chemical approach to the efficient generation of mouse embryonic stem cells
- Cloning technologies
- Andrew Fontes
- Stable transfection using episomal vectors to create modified human embryonic stem cells
- Ying Liu, Kate Judd, and Uma Lakshmipathy
- Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology
- Chad C. MacArthur
- Hossein Baharvand and Seyedeh-Nafiseh Hassani
- Review of the methods for human iPSC derivation
- Nasir Malik and Mahendra S. Rao
- Generation of human-induced pluripotent stem cells by lentiviral transduction
- Jennifer C. Moore
- Isbn
- 9781627033480
- Label
- Pluripotent stem cells : methods and protocols
- Title
- Pluripotent stem cells
- Title remainder
- methods and protocols
- Statement of responsibility
- edited by Uma Lakshmipathy, Mohan C. Vemuri
- Language
- eng
- Summary
- Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods, and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature
- Cataloging source
- SCB
- Dewey number
- 616.02/774
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QH588.S83
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
-
- W1
- QU 25
- NLM item number
- ME9616J v.997 2013
- http://library.link/vocab/relatedWorkOrContributorName
-
- Lakshmipathy, Uma
- Vemuri, Mohan C.
- Series statement
- Methods in molecular biology,
- Series volume
- v. no. 997
- http://library.link/vocab/subjectName
-
- Multipotent stem cells
- Embryonic stem cells
- Pluripotent Stem Cells
- Embryonic stem cells
- Multipotent stem cells
- Label
- Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus
- Pauline T. Lieu [and others]
- Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions
- Andrew Fontes [and others]
- Feeder-free substrates for pluripotent stem cell culture
- Alexandria Sams and Mark J. Powers
- Methods for culturing human embryonic stem cells on feeders
- Jasmeet Kaur and Mary Lynn Tilkins
- Methods for culturing human embryonic stem cells in a xeno-free system
- Jasmeet Kaur [and others]
- Sources and derivation of human embryonic stem cells
- Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage
- Dennis Van Hoof and Muluye E. Liku
- Directed differentiation of pluripotent stem cells to functional hepatocytes
- Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
- Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes
- Paul W. Burridge and Elias T. Zambidis
- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors
- Kejin Hu and Igor Slukvin
- Cellular characterization of human pluripotent stem cells
- Rene H. Quintanilla Jr.
- Michal Amit
- TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells
- Sunali N. Patel [and others]
- Epigenome analysis of pluripotent stem cells
- Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
- Single cell gene expression analysis of pluripotent stem cells
- Ronald V. Abruzzese and Richard A. Fekete
- Profiling stem cells using quantitative PCR protein assays
- David Ruff and Pauline T. Lieu
- Labeled stem cells as disease models and in drug discovery
- Catharina Ellerstrom, Raimund Strehl, and Johan Hyllner
- New chemical approach to the efficient generation of mouse embryonic stem cells
- Cloning technologies
- Andrew Fontes
- Stable transfection using episomal vectors to create modified human embryonic stem cells
- Ying Liu, Kate Judd, and Uma Lakshmipathy
- Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology
- Chad C. MacArthur
- Hossein Baharvand and Seyedeh-Nafiseh Hassani
- Review of the methods for human iPSC derivation
- Nasir Malik and Mahendra S. Rao
- Generation of human-induced pluripotent stem cells by lentiviral transduction
- Jennifer C. Moore
- Control code
- 837693702
- Extent
- 1 online resource (x, 292 pages)
- Form of item
- online
- Isbn
- 9781627033480
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1007/978-1-62703-348-0
- Other physical details
- illustrations (some color).
- Specific material designation
- remote
- System control number
- (OCoLC)837693702
- Label
- Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus
- Pauline T. Lieu [and others]
- Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions
- Andrew Fontes [and others]
- Feeder-free substrates for pluripotent stem cell culture
- Alexandria Sams and Mark J. Powers
- Methods for culturing human embryonic stem cells on feeders
- Jasmeet Kaur and Mary Lynn Tilkins
- Methods for culturing human embryonic stem cells in a xeno-free system
- Jasmeet Kaur [and others]
- Sources and derivation of human embryonic stem cells
- Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage
- Dennis Van Hoof and Muluye E. Liku
- Directed differentiation of pluripotent stem cells to functional hepatocytes
- Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
- Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes
- Paul W. Burridge and Elias T. Zambidis
- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors
- Kejin Hu and Igor Slukvin
- Cellular characterization of human pluripotent stem cells
- Rene H. Quintanilla Jr.
- Michal Amit
- TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells
- Sunali N. Patel [and others]
- Epigenome analysis of pluripotent stem cells
- Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
- Single cell gene expression analysis of pluripotent stem cells
- Ronald V. Abruzzese and Richard A. Fekete
- Profiling stem cells using quantitative PCR protein assays
- David Ruff and Pauline T. Lieu
- Labeled stem cells as disease models and in drug discovery
- Catharina Ellerstrom, Raimund Strehl, and Johan Hyllner
- New chemical approach to the efficient generation of mouse embryonic stem cells
- Cloning technologies
- Andrew Fontes
- Stable transfection using episomal vectors to create modified human embryonic stem cells
- Ying Liu, Kate Judd, and Uma Lakshmipathy
- Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology
- Chad C. MacArthur
- Hossein Baharvand and Seyedeh-Nafiseh Hassani
- Review of the methods for human iPSC derivation
- Nasir Malik and Mahendra S. Rao
- Generation of human-induced pluripotent stem cells by lentiviral transduction
- Jennifer C. Moore
- Control code
- 837693702
- Extent
- 1 online resource (x, 292 pages)
- Form of item
- online
- Isbn
- 9781627033480
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1007/978-1-62703-348-0
- Other physical details
- illustrations (some color).
- Specific material designation
- remote
- System control number
- (OCoLC)837693702
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Pluripotent-stem-cells--methods-and-protocols/uCwhkCt52HQ/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Pluripotent-stem-cells--methods-and-protocols/uCwhkCt52HQ/">Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
