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The Resource Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri

Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri

Label
Pluripotent stem cells : methods and protocols
Title
Pluripotent stem cells
Title remainder
methods and protocols
Statement of responsibility
edited by Uma Lakshmipathy, Mohan C. Vemuri
Contributor
Editor
Subject
Language
eng
Summary
Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods, and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature
Member of
Cataloging source
SCB
Dewey number
616.02/774
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QH588.S83
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.997 2013
http://library.link/vocab/relatedWorkOrContributorName
  • Lakshmipathy, Uma
  • Vemuri, Mohan C.
Series statement
Methods in molecular biology,
Series volume
v. no. 997
http://library.link/vocab/subjectName
  • Multipotent stem cells
  • Embryonic stem cells
  • Pluripotent Stem Cells
  • Embryonic stem cells
  • Multipotent stem cells
Label
Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri
Instantiates
Publication
Copyright
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus
  • Pauline T. Lieu [and others]
  • Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions
  • Andrew Fontes [and others]
  • Feeder-free substrates for pluripotent stem cell culture
  • Alexandria Sams and Mark J. Powers
  • Methods for culturing human embryonic stem cells on feeders
  • Jasmeet Kaur and Mary Lynn Tilkins
  • Methods for culturing human embryonic stem cells in a xeno-free system
  • Jasmeet Kaur [and others]
  • Sources and derivation of human embryonic stem cells
  • Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage
  • Dennis Van Hoof and Muluye E. Liku
  • Directed differentiation of pluripotent stem cells to functional hepatocytes
  • Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
  • Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes
  • Paul W. Burridge and Elias T. Zambidis
  • Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors
  • Kejin Hu and Igor Slukvin
  • Cellular characterization of human pluripotent stem cells
  • Rene H. Quintanilla Jr.
  • Michal Amit
  • TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells
  • Sunali N. Patel [and others]
  • Epigenome analysis of pluripotent stem cells
  • Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
  • Single cell gene expression analysis of pluripotent stem cells
  • Ronald V. Abruzzese and Richard A. Fekete
  • Profiling stem cells using quantitative PCR protein assays
  • David Ruff and Pauline T. Lieu
  • Labeled stem cells as disease models and in drug discovery
  • Catharina Ellerstrom, Raimund Strehl, and Johan Hyllner
  • New chemical approach to the efficient generation of mouse embryonic stem cells
  • Cloning technologies
  • Andrew Fontes
  • Stable transfection using episomal vectors to create modified human embryonic stem cells
  • Ying Liu, Kate Judd, and Uma Lakshmipathy
  • Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology
  • Chad C. MacArthur
  • Hossein Baharvand and Seyedeh-Nafiseh Hassani
  • Review of the methods for human iPSC derivation
  • Nasir Malik and Mahendra S. Rao
  • Generation of human-induced pluripotent stem cells by lentiviral transduction
  • Jennifer C. Moore
Control code
837693702
Extent
1 online resource (x, 292 pages)
Form of item
online
Isbn
9781627033480
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-348-0
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)837693702
Label
Pluripotent stem cells : methods and protocols, edited by Uma Lakshmipathy, Mohan C. Vemuri
Publication
Copyright
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus
  • Pauline T. Lieu [and others]
  • Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions
  • Andrew Fontes [and others]
  • Feeder-free substrates for pluripotent stem cell culture
  • Alexandria Sams and Mark J. Powers
  • Methods for culturing human embryonic stem cells on feeders
  • Jasmeet Kaur and Mary Lynn Tilkins
  • Methods for culturing human embryonic stem cells in a xeno-free system
  • Jasmeet Kaur [and others]
  • Sources and derivation of human embryonic stem cells
  • Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage
  • Dennis Van Hoof and Muluye E. Liku
  • Directed differentiation of pluripotent stem cells to functional hepatocytes
  • Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
  • Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes
  • Paul W. Burridge and Elias T. Zambidis
  • Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors
  • Kejin Hu and Igor Slukvin
  • Cellular characterization of human pluripotent stem cells
  • Rene H. Quintanilla Jr.
  • Michal Amit
  • TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells
  • Sunali N. Patel [and others]
  • Epigenome analysis of pluripotent stem cells
  • Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
  • Single cell gene expression analysis of pluripotent stem cells
  • Ronald V. Abruzzese and Richard A. Fekete
  • Profiling stem cells using quantitative PCR protein assays
  • David Ruff and Pauline T. Lieu
  • Labeled stem cells as disease models and in drug discovery
  • Catharina Ellerstrom, Raimund Strehl, and Johan Hyllner
  • New chemical approach to the efficient generation of mouse embryonic stem cells
  • Cloning technologies
  • Andrew Fontes
  • Stable transfection using episomal vectors to create modified human embryonic stem cells
  • Ying Liu, Kate Judd, and Uma Lakshmipathy
  • Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology
  • Chad C. MacArthur
  • Hossein Baharvand and Seyedeh-Nafiseh Hassani
  • Review of the methods for human iPSC derivation
  • Nasir Malik and Mahendra S. Rao
  • Generation of human-induced pluripotent stem cells by lentiviral transduction
  • Jennifer C. Moore
Control code
837693702
Extent
1 online resource (x, 292 pages)
Form of item
online
Isbn
9781627033480
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-348-0
Other physical details
illustrations (some color).
Specific material designation
remote
System control number
(OCoLC)837693702

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