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The Resource Protein NMR techniques, edited by A. Kristina Downing

Protein NMR techniques, edited by A. Kristina Downing

Label
Protein NMR techniques
Title
Protein NMR techniques
Statement of responsibility
edited by A. Kristina Downing
Contributor
Subject
Language
eng
Summary
Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules
Member of
Cataloging source
COO
Dewey number
572/.6
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP551
LC item number
.P3976 2004
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 55
NLM item number
  • ME9616J v.278 2004
  • P9672 2004
http://library.link/vocab/relatedWorkOrContributorName
Downing, A. Kristina
Series statement
Methods in molecular biology
Series volume
278
http://library.link/vocab/subjectName
  • Proteins
  • Nuclear magnetic resonance spectroscopy
  • Proteins
  • Automation, Laboratory
  • Nuclear Magnetic Resonance, Biomolecular
  • SCIENCE
  • Nuclear magnetic resonance spectroscopy
  • Proteins
Label
Protein NMR techniques, edited by A. Kristina Downing
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Screening and optimizing protein production in e. coli / Lorraine Hewitt and James M. McDonnell -- Isotopic labelling of recombinant proteins from the methylotrophic yeast pichia pastoris / Andrew R. Pickford and Joanne M. O'Leary -- Perdeuteration/site-specific protonation approaches for high mw -- Proteins / Stephen Matthews -- Segmental isotopic labelling for structural biological applications of NMR / David Cowburn [and others] -- TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins / Guang Zhu [and others]
  • Media for studies of partially aligned states / Kieran Fleming and Stephen Matthews -- Residual dipolar couplings in protein structure determination / Eva de Alba and Nico Tjandra -- Projection angle restraints for studying structure and dynamics of biomolecules / Christian Griesinger [and others] -- Characterizing domain interfaces by NMR / Luke M. Rooney, Sachchidanand, and Jörn M. Werner -- Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations / Jennifer Blake Hall, Oliver Walker, and David Fushman
  • TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding / Guang Zhu [and others] -- Measurement of intermediate exchange phenomena / James G. Kempf and J. Patrick Loria -- NMR studies of partially folded molten globule states / Christina Redfield -- Structure determination of protein complexes by NMR / Daniel Nietlispach [and others] -- NMR studies of protein/nucleic acid interactions / Gabriele Varani, Yu Chen, and Thomas C. Leeper -- Using NMRview to visualize and analyze the NMR spectra of macromolecules / Bruce A. Johnson
  • Automated NMR structure calculation with CYANA / Peter Güntert -- NOE assignment with ARIA 2.0: the nuts and bolts / Michael Habeck [and others] -- Membrane protein structure determinations using solid state NMR / Anthony Watts [and others]
Control code
61264161
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xi, 487 pages)
Form of item
online
Isbn
9781592598090
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)61264161
Label
Protein NMR techniques, edited by A. Kristina Downing
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Screening and optimizing protein production in e. coli / Lorraine Hewitt and James M. McDonnell -- Isotopic labelling of recombinant proteins from the methylotrophic yeast pichia pastoris / Andrew R. Pickford and Joanne M. O'Leary -- Perdeuteration/site-specific protonation approaches for high mw -- Proteins / Stephen Matthews -- Segmental isotopic labelling for structural biological applications of NMR / David Cowburn [and others] -- TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins / Guang Zhu [and others]
  • Media for studies of partially aligned states / Kieran Fleming and Stephen Matthews -- Residual dipolar couplings in protein structure determination / Eva de Alba and Nico Tjandra -- Projection angle restraints for studying structure and dynamics of biomolecules / Christian Griesinger [and others] -- Characterizing domain interfaces by NMR / Luke M. Rooney, Sachchidanand, and Jörn M. Werner -- Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations / Jennifer Blake Hall, Oliver Walker, and David Fushman
  • TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding / Guang Zhu [and others] -- Measurement of intermediate exchange phenomena / James G. Kempf and J. Patrick Loria -- NMR studies of partially folded molten globule states / Christina Redfield -- Structure determination of protein complexes by NMR / Daniel Nietlispach [and others] -- NMR studies of protein/nucleic acid interactions / Gabriele Varani, Yu Chen, and Thomas C. Leeper -- Using NMRview to visualize and analyze the NMR spectra of macromolecules / Bruce A. Johnson
  • Automated NMR structure calculation with CYANA / Peter Güntert -- NOE assignment with ARIA 2.0: the nuts and bolts / Michael Habeck [and others] -- Membrane protein structure determinations using solid state NMR / Anthony Watts [and others]
Control code
61264161
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xi, 487 pages)
Form of item
online
Isbn
9781592598090
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)61264161

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