The Resource Protein NMR techniques, edited by A. Kristina Downing
Protein NMR techniques, edited by A. Kristina Downing
Resource Information
The item Protein NMR techniques, edited by A. Kristina Downing represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Protein NMR techniques, edited by A. Kristina Downing represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules
- Language
- eng
- Edition
- 2nd ed.
- Extent
- 1 online resource (xi, 487 pages)
- Contents
-
- Screening and optimizing protein production in e. coli / Lorraine Hewitt and James M. McDonnell -- Isotopic labelling of recombinant proteins from the methylotrophic yeast pichia pastoris / Andrew R. Pickford and Joanne M. O'Leary -- Perdeuteration/site-specific protonation approaches for high mw -- Proteins / Stephen Matthews -- Segmental isotopic labelling for structural biological applications of NMR / David Cowburn [and others] -- TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins / Guang Zhu [and others]
- Media for studies of partially aligned states / Kieran Fleming and Stephen Matthews -- Residual dipolar couplings in protein structure determination / Eva de Alba and Nico Tjandra -- Projection angle restraints for studying structure and dynamics of biomolecules / Christian Griesinger [and others] -- Characterizing domain interfaces by NMR / Luke M. Rooney, Sachchidanand, and Jörn M. Werner -- Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations / Jennifer Blake Hall, Oliver Walker, and David Fushman
- TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding / Guang Zhu [and others] -- Measurement of intermediate exchange phenomena / James G. Kempf and J. Patrick Loria -- NMR studies of partially folded molten globule states / Christina Redfield -- Structure determination of protein complexes by NMR / Daniel Nietlispach [and others] -- NMR studies of protein/nucleic acid interactions / Gabriele Varani, Yu Chen, and Thomas C. Leeper -- Using NMRview to visualize and analyze the NMR spectra of macromolecules / Bruce A. Johnson
- Automated NMR structure calculation with CYANA / Peter Güntert -- NOE assignment with ARIA 2.0: the nuts and bolts / Michael Habeck [and others] -- Membrane protein structure determinations using solid state NMR / Anthony Watts [and others]
- Isbn
- 9781592598090
- Label
- Protein NMR techniques
- Title
- Protein NMR techniques
- Statement of responsibility
- edited by A. Kristina Downing
- Subject
-
- Automation, Laboratory
- Nuclear Magnetic Resonance, Biomolecular -- methods
- Nuclear magnetic resonance spectroscopy
- Nuclear magnetic resonance spectroscopy
- Nuclear magnetic resonance spectroscopy
- Proteins -- Analysis
- Proteins -- Analysis
- Proteins -- Analysis
- Proteins -- analysis
- SCIENCE -- Life Sciences | Biochemistry
- Language
- eng
- Summary
- Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules
- Cataloging source
- COO
- Dewey number
- 572/.6
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QP551
- LC item number
- .P3976 2004
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
-
- W1
- QU 55
- NLM item number
-
- ME9616J v.278 2004
- P9672 2004
- http://library.link/vocab/relatedWorkOrContributorName
- Downing, A. Kristina
- Series statement
- Methods in molecular biology
- Series volume
- 278
- http://library.link/vocab/subjectName
-
- Proteins
- Nuclear magnetic resonance spectroscopy
- Proteins
- Automation, Laboratory
- Nuclear Magnetic Resonance, Biomolecular
- SCIENCE
- Nuclear magnetic resonance spectroscopy
- Proteins
- Label
- Protein NMR techniques, edited by A. Kristina Downing
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Screening and optimizing protein production in e. coli / Lorraine Hewitt and James M. McDonnell -- Isotopic labelling of recombinant proteins from the methylotrophic yeast pichia pastoris / Andrew R. Pickford and Joanne M. O'Leary -- Perdeuteration/site-specific protonation approaches for high mw -- Proteins / Stephen Matthews -- Segmental isotopic labelling for structural biological applications of NMR / David Cowburn [and others] -- TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins / Guang Zhu [and others]
- Media for studies of partially aligned states / Kieran Fleming and Stephen Matthews -- Residual dipolar couplings in protein structure determination / Eva de Alba and Nico Tjandra -- Projection angle restraints for studying structure and dynamics of biomolecules / Christian Griesinger [and others] -- Characterizing domain interfaces by NMR / Luke M. Rooney, Sachchidanand, and Jörn M. Werner -- Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations / Jennifer Blake Hall, Oliver Walker, and David Fushman
- TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding / Guang Zhu [and others] -- Measurement of intermediate exchange phenomena / James G. Kempf and J. Patrick Loria -- NMR studies of partially folded molten globule states / Christina Redfield -- Structure determination of protein complexes by NMR / Daniel Nietlispach [and others] -- NMR studies of protein/nucleic acid interactions / Gabriele Varani, Yu Chen, and Thomas C. Leeper -- Using NMRview to visualize and analyze the NMR spectra of macromolecules / Bruce A. Johnson
- Automated NMR structure calculation with CYANA / Peter Güntert -- NOE assignment with ARIA 2.0: the nuts and bolts / Michael Habeck [and others] -- Membrane protein structure determinations using solid state NMR / Anthony Watts [and others]
- Control code
- 61264161
- Dimensions
- unknown
- Edition
- 2nd ed.
- Extent
- 1 online resource (xi, 487 pages)
- Form of item
- online
- Isbn
- 9781592598090
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations.
- Specific material designation
- remote
- System control number
- (OCoLC)61264161
- Label
- Protein NMR techniques, edited by A. Kristina Downing
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Screening and optimizing protein production in e. coli / Lorraine Hewitt and James M. McDonnell -- Isotopic labelling of recombinant proteins from the methylotrophic yeast pichia pastoris / Andrew R. Pickford and Joanne M. O'Leary -- Perdeuteration/site-specific protonation approaches for high mw -- Proteins / Stephen Matthews -- Segmental isotopic labelling for structural biological applications of NMR / David Cowburn [and others] -- TROSY-based correlation and NOE spectroscopy for NMR structural studies of large proteins / Guang Zhu [and others]
- Media for studies of partially aligned states / Kieran Fleming and Stephen Matthews -- Residual dipolar couplings in protein structure determination / Eva de Alba and Nico Tjandra -- Projection angle restraints for studying structure and dynamics of biomolecules / Christian Griesinger [and others] -- Characterizing domain interfaces by NMR / Luke M. Rooney, Sachchidanand, and Jörn M. Werner -- Characterization of the overall rotational diffusion of a protein from 15N relaxation measurements and hydrodynamic calculations / Jennifer Blake Hall, Oliver Walker, and David Fushman
- TROSY-based NMR experiments for the study of macromolecular dynamics and hydrogen bonding / Guang Zhu [and others] -- Measurement of intermediate exchange phenomena / James G. Kempf and J. Patrick Loria -- NMR studies of partially folded molten globule states / Christina Redfield -- Structure determination of protein complexes by NMR / Daniel Nietlispach [and others] -- NMR studies of protein/nucleic acid interactions / Gabriele Varani, Yu Chen, and Thomas C. Leeper -- Using NMRview to visualize and analyze the NMR spectra of macromolecules / Bruce A. Johnson
- Automated NMR structure calculation with CYANA / Peter Güntert -- NOE assignment with ARIA 2.0: the nuts and bolts / Michael Habeck [and others] -- Membrane protein structure determinations using solid state NMR / Anthony Watts [and others]
- Control code
- 61264161
- Dimensions
- unknown
- Edition
- 2nd ed.
- Extent
- 1 online resource (xi, 487 pages)
- Form of item
- online
- Isbn
- 9781592598090
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations.
- Specific material designation
- remote
- System control number
- (OCoLC)61264161
Subject
- Automation, Laboratory
- Nuclear Magnetic Resonance, Biomolecular -- methods
- Nuclear magnetic resonance spectroscopy
- Nuclear magnetic resonance spectroscopy
- Nuclear magnetic resonance spectroscopy
- Proteins -- Analysis
- Proteins -- Analysis
- Proteins -- Analysis
- Proteins -- analysis
- SCIENCE -- Life Sciences | Biochemistry
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/Protein-NMR-techniques-edited-by-A.-Kristina/WIQhgT8QDL4/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/Protein-NMR-techniques-edited-by-A.-Kristina/WIQhgT8QDL4/">Protein NMR techniques, edited by A. Kristina Downing</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
