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The Resource Protein purification protocols, edited by Shawn Doonan

Protein purification protocols, edited by Shawn Doonan

Label
Protein purification protocols
Title
Protein purification protocols
Statement of responsibility
edited by Shawn Doonan
Contributor
Subject
Genre
Language
eng
Summary
A comprehensive collection of essential, time-tested recipes for successful protein fractionation and purification in any experimental circumstance. The protocols give step-by-step instructions on how to select a source for the protein of interest, how to obtain a usable initial extract, how to purify the protein from that extract using both chemical and molecular methods, and how to dry and store the purified protein. Protein Purification Protocols provides all that is needed to design and carry out a successful purification program. It helps both experienced and novice investigators to clarify and define their purification problems and then provides a comprehensive set of tools for a practical solution
Member of
Action
digitized
Cataloging source
YNG
Dewey number
547.7/5046
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP551
LC item number
.P69756 1996
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
v.59
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.59 1996
  • P967 1996
http://library.link/vocab/relatedWorkOrContributorName
Doonan, Shawn
Series statement
Methods in molecular biology
Series volume
v. 59
http://library.link/vocab/subjectName
  • Proteins
  • Proteins
  • Proteins
  • Eiwitten
  • Zuiveringstechnieken
  • Extractie (scheikunde)
  • Affiniteitschromatografie
  • Chromatografie
  • Wetenschappelijke technieken
  • Animal Biochemistry
  • Human Anatomy & Physiology
  • Health & Biological Sciences
Target audience
adult
Label
Protein purification protocols, edited by Shawn Doonan
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
General strategies / Shawn Doonan -- Preparation of extracts from animal tissues / Shawn Doonan -- Protein extraction from plant tissues / Peter R. Shewry, Roger J. Fido -- Extraction of recombinant protein from bacteria / D. Margaret Worrall -- Protein extraction from fungi / Paul Bridge -- Subcellular fractionation of animal tissues / Norma M. Ryan -- Subcellular fractionation of plant tissues : isolation of chloroplasts and mitochondria from leaves / Alyson K. Tobin -- The extraction of enzymes from plant tissues rich in phenolic compounds / William S. Pierpoint -- Avoidance of proteolysis in extracts / Robert J. Beynon, Simon Oliver -- Concentration of extracts / Shawn Doonan -- Making and changing buffers / Shawn Doonan -- Purification and concentration by ultrafiltration / Paul Schratter -- Bulk purification by fractional precipitation / Shawn Doonan -- Ion-exchange chromatography / David Sheehan, Richard FitzGerald -- Hydrophobic interaction chromatography / Paul O'Farrell -- Affinity chromatography / Paul Cutler -- Dye-ligand affinity chromatography / D. Margaret Worrall -- Lectin affinity chromatography / Iris West, Owen Goldring -- Immunoaffinity chromatography / George W. Jack, David J. Beer -- Immobilized metal ion affinity chromatography / Tai-Tung Yip, T. William Hutchens -- Chromatography on hydroxyapatite / Shawn Doonan -- Affinity precipitation methods / Jane A. Irwin, Keith F. Tipton -- Isoelectric focusing / Reiner Westermeier -- Chromatofocusing / Timothy J. Mantle, Patricia Noone -- Size-exclusion chromatography / Paul Cutler -- Fast protein liquid chromatography (FPLC) methods / David Sheehan -- Reversed-phase chromatography of proteins / Bill Neville -- Extraction of membrane proteins / Kay Ohlendieck -- Removal of detergent from protein fractions / Kay Ohlendieck -- Purification of membrane proteins / Kay Ohlendieck -- Lyophilization of proteins / Ciarán Ó. Fágáin -- Storage of pure proteins / Ciarán Ó. Fágáin -- Electroelution of proteins from polyacrylamide gels / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels / Michael J. Dunn -- High-performance electrophoresis chromatography / Serge Desnoyers, Sylvie Bourassa, Guy G. Poirier -- Practical column chromatography / Shawn Doonan
Control code
260242047
Dimensions
unknown
Extent
1 online resource (x, 405 pages)
File format
one file format
Form of item
online
Isbn
9781280836664
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896033368
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)260242047
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Protein purification protocols, edited by Shawn Doonan
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
General strategies / Shawn Doonan -- Preparation of extracts from animal tissues / Shawn Doonan -- Protein extraction from plant tissues / Peter R. Shewry, Roger J. Fido -- Extraction of recombinant protein from bacteria / D. Margaret Worrall -- Protein extraction from fungi / Paul Bridge -- Subcellular fractionation of animal tissues / Norma M. Ryan -- Subcellular fractionation of plant tissues : isolation of chloroplasts and mitochondria from leaves / Alyson K. Tobin -- The extraction of enzymes from plant tissues rich in phenolic compounds / William S. Pierpoint -- Avoidance of proteolysis in extracts / Robert J. Beynon, Simon Oliver -- Concentration of extracts / Shawn Doonan -- Making and changing buffers / Shawn Doonan -- Purification and concentration by ultrafiltration / Paul Schratter -- Bulk purification by fractional precipitation / Shawn Doonan -- Ion-exchange chromatography / David Sheehan, Richard FitzGerald -- Hydrophobic interaction chromatography / Paul O'Farrell -- Affinity chromatography / Paul Cutler -- Dye-ligand affinity chromatography / D. Margaret Worrall -- Lectin affinity chromatography / Iris West, Owen Goldring -- Immunoaffinity chromatography / George W. Jack, David J. Beer -- Immobilized metal ion affinity chromatography / Tai-Tung Yip, T. William Hutchens -- Chromatography on hydroxyapatite / Shawn Doonan -- Affinity precipitation methods / Jane A. Irwin, Keith F. Tipton -- Isoelectric focusing / Reiner Westermeier -- Chromatofocusing / Timothy J. Mantle, Patricia Noone -- Size-exclusion chromatography / Paul Cutler -- Fast protein liquid chromatography (FPLC) methods / David Sheehan -- Reversed-phase chromatography of proteins / Bill Neville -- Extraction of membrane proteins / Kay Ohlendieck -- Removal of detergent from protein fractions / Kay Ohlendieck -- Purification of membrane proteins / Kay Ohlendieck -- Lyophilization of proteins / Ciarán Ó. Fágáin -- Storage of pure proteins / Ciarán Ó. Fágáin -- Electroelution of proteins from polyacrylamide gels / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels / Michael J. Dunn -- High-performance electrophoresis chromatography / Serge Desnoyers, Sylvie Bourassa, Guy G. Poirier -- Practical column chromatography / Shawn Doonan
Control code
260242047
Dimensions
unknown
Extent
1 online resource (x, 405 pages)
File format
one file format
Form of item
online
Isbn
9781280836664
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896033368
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)260242047
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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