Coverart for item
The Resource Protocols for gene analysis, edited by Adrian J. Harwood

Protocols for gene analysis, edited by Adrian J. Harwood

Label
Protocols for gene analysis
Title
Protocols for gene analysis
Statement of responsibility
edited by Adrian J. Harwood
Contributor
Subject
Language
eng
Member of
Cataloging source
DLC
Dewey number
574.87/322
Illustrations
illustrations
Index
index present
LC call number
QH445.2
LC item number
.P76 1994
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
no.31
Nature of contents
bibliography
NLM call number
  • W1
  • QH 445.2
NLM item number
  • ME9616J v.31 1994
  • P967 1994
http://library.link/vocab/relatedWorkOrContributorName
Harwood, Adrian J
Series statement
Methods in molecular biology
Series volume
31
http://library.link/vocab/subjectName
  • Gene mapping
  • Nucleotide sequence
  • Base Sequence
  • Chromosome Mapping
  • Genanalyse
  • Wetenschappelijke technieken
Label
Protocols for gene analysis, edited by Adrian J. Harwood
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Basic recombinant DNA techniques -- Transformation of bacteria by electroporation -- Direct cloning of [lambda]g11 cDNA inserts into a plasmid vector -- PCR cloning using T-vectors -- Thermal cycle dideoxy DNA sequencing -- In vitro mutagenesis -- Ordered deletions using exonuclease III -- Site-directed mutagenesis using a double-stranded DNA template -- Site-directed mutagenesis using a uracil-containing phagemid template -- Construction of linker-scanning mutations by oligonucleotide ligation -- Construction of linker scanning mutations using the polymerase chain reaction -- Localized random polymerase chain reaction mutagenesis -- Genomic structure -- Simultaneous isolation of RNA and DNA from tissues and cultured cells -- Physical mapping of the human genome by pulsed field gel electrophoresis -- Field inversion gel electrophoresis -- Enhanced chemiluminescent detection of horseradish peroxidase labeled probes -- Nonradioactive oligonucleotide probe labeling -- Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels -- Inverse polymerase chain reaction -- Sequence variations -- Use of silver staining to detect nucleic acids -- Nonradioactive method for the detection of single-strand conformational polymorphisms (SSCP) -- Temperature gradient gel electrophoresis (TGGE) for the detection of polymorphic DNA and RNA -- TGGE in quantitative PCR of DNA and RNA -- PGK-PCR clonality assay (PPCA) -- Direct sequencing of PCR products -- Gene expression -- Use of riboprobes for the analysis of gene expression -- Quantification of absolute amounts of cellular messenger RNA by RNA-excess solution hybridization -- Measurements of rate of transcription in isolated nuclei by nuclear run-off assay -- RNA polymerase II in vitro transcription system -- S1 mapping using single-stranded DNA probes -- Single primer-mediated polymerase chain reaction -- Protein-DNA interactions -- In vivo DNA footprinting by linear amplification -- DNA photofootprinting with Rh(phi)2bpy3+ -- Gel retardation assay -- Southwestern assay -- Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes -- Protein function -- 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification -- Production of 35S-labeled proteins in E. coli and their use as molecular probes -- Preparation and ligand screening of a [lambda]g11 lysogen library
Control code
29702544
Dimensions
23 cm
Extent
xiv, 411 pages
Isbn
9780896032583
Lccn
94002365
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
System control number
(WaOLN)1670621
Label
Protocols for gene analysis, edited by Adrian J. Harwood
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
  • nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Basic recombinant DNA techniques -- Transformation of bacteria by electroporation -- Direct cloning of [lambda]g11 cDNA inserts into a plasmid vector -- PCR cloning using T-vectors -- Thermal cycle dideoxy DNA sequencing -- In vitro mutagenesis -- Ordered deletions using exonuclease III -- Site-directed mutagenesis using a double-stranded DNA template -- Site-directed mutagenesis using a uracil-containing phagemid template -- Construction of linker-scanning mutations by oligonucleotide ligation -- Construction of linker scanning mutations using the polymerase chain reaction -- Localized random polymerase chain reaction mutagenesis -- Genomic structure -- Simultaneous isolation of RNA and DNA from tissues and cultured cells -- Physical mapping of the human genome by pulsed field gel electrophoresis -- Field inversion gel electrophoresis -- Enhanced chemiluminescent detection of horseradish peroxidase labeled probes -- Nonradioactive oligonucleotide probe labeling -- Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels -- Inverse polymerase chain reaction -- Sequence variations -- Use of silver staining to detect nucleic acids -- Nonradioactive method for the detection of single-strand conformational polymorphisms (SSCP) -- Temperature gradient gel electrophoresis (TGGE) for the detection of polymorphic DNA and RNA -- TGGE in quantitative PCR of DNA and RNA -- PGK-PCR clonality assay (PPCA) -- Direct sequencing of PCR products -- Gene expression -- Use of riboprobes for the analysis of gene expression -- Quantification of absolute amounts of cellular messenger RNA by RNA-excess solution hybridization -- Measurements of rate of transcription in isolated nuclei by nuclear run-off assay -- RNA polymerase II in vitro transcription system -- S1 mapping using single-stranded DNA probes -- Single primer-mediated polymerase chain reaction -- Protein-DNA interactions -- In vivo DNA footprinting by linear amplification -- DNA photofootprinting with Rh(phi)2bpy3+ -- Gel retardation assay -- Southwestern assay -- Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes -- Protein function -- 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification -- Production of 35S-labeled proteins in E. coli and their use as molecular probes -- Preparation and ligand screening of a [lambda]g11 lysogen library
Control code
29702544
Dimensions
23 cm
Extent
xiv, 411 pages
Isbn
9780896032583
Lccn
94002365
Media category
unmediated
Media MARC source
rdamedia
Media type code
  • n
Other physical details
illustrations
System control number
(WaOLN)1670621

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