The Resource Protocols for gene analysis, edited by Adrian J. Harwood
Protocols for gene analysis, edited by Adrian J. Harwood
Resource Information
The item Protocols for gene analysis, edited by Adrian J. Harwood represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item Protocols for gene analysis, edited by Adrian J. Harwood represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts
- Language
- eng
- Extent
- 1 online resource (xiv, 411 pages)
- Contents
-
- Basic recombinant DNA techniques
- Transformation of bacteria by electroporation
- Direct cloning of [lambda]g11 cDNA inserts into a plasmid vector
- PCR cloning using T-vectors
- Thermal cycle dideoxy DNA sequencing
- In vitro mutagenesis
- Ordered deletions using exonuclease III
- Site-directed mutagenesis using a double-stranded DNA template
- Site-directed mutagenesis using a uracil-containing phagemid template
- Construction of linker-scanning mutations by oligonucleotide ligation
- Construction of linker scanning mutations using the polymerase chain reaction
- Localized random polymerase chain reaction mutagenesis
- Genomic structure
- Simultaneous isolation of RNA and DNA from tissues and cultured cells
- Physical mapping of the human genome by pulsed field gel electrophoresis
- Field inversion gel electrophoresis
- Enhanced chemiluminescent detection of horseradish peroxidase labeled probes
- Nonradioactive oligonucleotide probe labeling
- Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels
- Inverse polymerase chain reaction
- Sequence variations
- Use of silver staining to detect nucleic acids
- Nonradioactive method for the detection of single-strand conformational polymorphisms (SSCP)
- Temperature gradient gel electrophoresis (TGGE) for the detection of polymorphic DNA and RNA
- TGGE in quantitative PCR of DNA and RNA
- PGK-PCR clonality assay (PPCA)
- Direct sequencing of PCR products
- Gene expression
- Use of riboprobes for the analysis of gene expression
- Quantification of absolute amounts of cellular messenger RNA by RNA-excess solution hybridization
- Measurements of rate of transcription in isolated nuclei by nuclear run-off assay
- RNA polymerase II in vitro transcription system
- S1 mapping using single-stranded DNA probes
- Single primer-mediated polymerase chain reaction
- Protein-DNA interactions
- In vivo DNA footprinting by linear amplification
- DNA photofootprinting with Rh(phi)2bpy3
- Gel retardation assay
- Southwestern assay
- Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes
- Protein function
- 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification
- Production of 35S-labeled proteins in E. coli and their use as molecular probes
- Preparation and ligand screening of a [lambda]g11 lysogen library
- Isbn
- 9781592595181
- Label
- Protocols for gene analysis
- Title
- Protocols for gene analysis
- Statement of responsibility
- edited by Adrian J. Harwood
- Language
- eng
- Summary
- This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts
- Cataloging source
- YNG
- Dewey number
- 574.87/322
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QH445.2
- LC item number
- .P76 1994
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
-
- W1
- QH 445.2
- NLM item number
-
- ME9616J v.31 1994
- P967 1994
- http://library.link/vocab/relatedWorkOrContributorName
- Harwood, Adrian J
- Series statement
- Methods in molecular biology
- Series volume
- v. 31
- http://library.link/vocab/subjectName
-
- Gene mapping
- Nucleotide sequence
- Chromosome Mapping
- Base Sequence
- Gene mapping
- Nucleotide sequence
- Label
- Protocols for gene analysis, edited by Adrian J. Harwood
- Antecedent source
- unknown
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- Basic recombinant DNA techniques -- Transformation of bacteria by electroporation -- Direct cloning of [lambda]g11 cDNA inserts into a plasmid vector -- PCR cloning using T-vectors -- Thermal cycle dideoxy DNA sequencing -- In vitro mutagenesis -- Ordered deletions using exonuclease III -- Site-directed mutagenesis using a double-stranded DNA template -- Site-directed mutagenesis using a uracil-containing phagemid template -- Construction of linker-scanning mutations by oligonucleotide ligation -- Construction of linker scanning mutations using the polymerase chain reaction -- Localized random polymerase chain reaction mutagenesis -- Genomic structure -- Simultaneous isolation of RNA and DNA from tissues and cultured cells -- Physical mapping of the human genome by pulsed field gel electrophoresis -- Field inversion gel electrophoresis -- Enhanced chemiluminescent detection of horseradish peroxidase labeled probes -- Nonradioactive oligonucleotide probe labeling -- Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels -- Inverse polymerase chain reaction -- Sequence variations -- Use of silver staining to detect nucleic acids -- Nonradioactive method for the detection of single-strand conformational polymorphisms (SSCP) -- Temperature gradient gel electrophoresis (TGGE) for the detection of polymorphic DNA and RNA -- TGGE in quantitative PCR of DNA and RNA -- PGK-PCR clonality assay (PPCA) -- Direct sequencing of PCR products -- Gene expression -- Use of riboprobes for the analysis of gene expression -- Quantification of absolute amounts of cellular messenger RNA by RNA-excess solution hybridization -- Measurements of rate of transcription in isolated nuclei by nuclear run-off assay -- RNA polymerase II in vitro transcription system -- S1 mapping using single-stranded DNA probes -- Single primer-mediated polymerase chain reaction -- Protein-DNA interactions -- In vivo DNA footprinting by linear amplification -- DNA photofootprinting with Rh(phi)2bpy3 -- Gel retardation assay -- Southwestern assay -- Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes -- Protein function -- 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification -- Production of 35S-labeled proteins in E. coli and their use as molecular probes -- Preparation and ligand screening of a [lambda]g11 lysogen library
- Control code
- 260242057
- Dimensions
- unknown
- Extent
- 1 online resource (xiv, 411 pages)
- File format
- one file format
- Form of item
- online
- Isbn
- 9781592595181
- Level of compression
- unknown
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations.
- Quality assurance targets
- unknown
- Reformatting quality
- access
- Specific material designation
- remote
- System control number
- (OCoLC)260242057
- Label
- Protocols for gene analysis, edited by Adrian J. Harwood
- Antecedent source
- unknown
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- Basic recombinant DNA techniques -- Transformation of bacteria by electroporation -- Direct cloning of [lambda]g11 cDNA inserts into a plasmid vector -- PCR cloning using T-vectors -- Thermal cycle dideoxy DNA sequencing -- In vitro mutagenesis -- Ordered deletions using exonuclease III -- Site-directed mutagenesis using a double-stranded DNA template -- Site-directed mutagenesis using a uracil-containing phagemid template -- Construction of linker-scanning mutations by oligonucleotide ligation -- Construction of linker scanning mutations using the polymerase chain reaction -- Localized random polymerase chain reaction mutagenesis -- Genomic structure -- Simultaneous isolation of RNA and DNA from tissues and cultured cells -- Physical mapping of the human genome by pulsed field gel electrophoresis -- Field inversion gel electrophoresis -- Enhanced chemiluminescent detection of horseradish peroxidase labeled probes -- Nonradioactive oligonucleotide probe labeling -- Analysis of DNA restriction enzyme digests by two-dimensional electrophoresis in agarose gels -- Inverse polymerase chain reaction -- Sequence variations -- Use of silver staining to detect nucleic acids -- Nonradioactive method for the detection of single-strand conformational polymorphisms (SSCP) -- Temperature gradient gel electrophoresis (TGGE) for the detection of polymorphic DNA and RNA -- TGGE in quantitative PCR of DNA and RNA -- PGK-PCR clonality assay (PPCA) -- Direct sequencing of PCR products -- Gene expression -- Use of riboprobes for the analysis of gene expression -- Quantification of absolute amounts of cellular messenger RNA by RNA-excess solution hybridization -- Measurements of rate of transcription in isolated nuclei by nuclear run-off assay -- RNA polymerase II in vitro transcription system -- S1 mapping using single-stranded DNA probes -- Single primer-mediated polymerase chain reaction -- Protein-DNA interactions -- In vivo DNA footprinting by linear amplification -- DNA photofootprinting with Rh(phi)2bpy3 -- Gel retardation assay -- Southwestern assay -- Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes -- Protein function -- 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification -- Production of 35S-labeled proteins in E. coli and their use as molecular probes -- Preparation and ligand screening of a [lambda]g11 lysogen library
- Control code
- 260242057
- Dimensions
- unknown
- Extent
- 1 online resource (xiv, 411 pages)
- File format
- one file format
- Form of item
- online
- Isbn
- 9781592595181
- Level of compression
- unknown
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations.
- Quality assurance targets
- unknown
- Reformatting quality
- access
- Specific material designation
- remote
- System control number
- (OCoLC)260242057
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