Coverart for item
The Resource Protocols in molecular neurobiology, edited by Alan Longstaff, Patricia Revest

Protocols in molecular neurobiology, edited by Alan Longstaff, Patricia Revest

Label
Protocols in molecular neurobiology
Title
Protocols in molecular neurobiology
Statement of responsibility
edited by Alan Longstaff, Patricia Revest
Contributor
Subject
Genre
Language
eng
Summary
Neurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical* or "molecular biological* one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literature- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appreciate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the information necessary to reproduce the technique and its results in any appropriately equipped laboratory
Member of
Action
digitized
Cataloging source
YNG
Dewey number
599/.0188
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP356.2
LC item number
.P76 1992
Literary form
non fiction
NAL call number
QH506.M45
NAL item number
no.13
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QP 356.2
NLM item number
  • ME9616J v.13 1992
  • P967 1992
http://library.link/vocab/relatedWorkOrContributorName
  • Longstaff, Alan
  • Revest, Patricia
Series statement
Methods in molecular biology
Series volume
v. 13
http://library.link/vocab/subjectName
  • Molecular neurobiology
  • Neurobiology
  • Molecular neurobiology
  • Moleculaire biologie
  • Zenuwstelsel
  • Wetenschappelijke technieken
Target audience
adult
Label
Protocols in molecular neurobiology, edited by Alan Longstaff, Patricia Revest
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Separation of large DNA molecules by pulsed-field gel electrophoresis / Duncan J. Shaw -- Isolation of genomic DNA from invertebrates / Erno Vreugdenhil and Mark G. Darlison -- Analysis of mitochondrial DNA mutations / Masashi Tanaka and Takayuki Ozawa -- Design and use of oligonucleotides / Alan N. Bateson and Mark G. Darlison -- Use of degenerate oligonucleotides for polymerase chain-reaction-based isolation of related DNA sequences / Tania E. Webb and Alan N. Bateson -- Isolation of photoreceptor cell-specific MEKA cDNA by differential hybridization / Che-Hui Kuo -- Generation of isoform-specific antisera from cloned cDNAs: application to multiple forms of the Na/K-ATPase expressed in rat brain / Andrew W. Shyjan and Robert Levenson -- Determination of transcriptional intitiation sites and their usage in the nervous system / Gary Weisinger, Joseph D. DeCristofaro and Edmund F. La Gamma -- Mapping of trans-acting factor binding in the nervous system / Gary Weisinger and Edmund F. La Gamma -- In situ hybridization to brain tissue sections using labeled single-strand complementary RNA probes / Giulio M. Pasinetti -- In situ hybridization histochemistry using alkaline phosphatase-labeled oligodeoxynucleotide probe / Hiroshi Kiyama, Piers C. Emson and Masaya Tohyama -- Identification of neuropeptide gene regulatory elements in transgenic mice / Malcolm J. Low -- Expression of exogenous ion channels and neurotransmitter receptors in RNA-injected Xenopus oocytes / Nathan Dascal and Ilana Lotan -- Analysis of insulin and insulin-like growth factor-I receptors in neural tissues / Martin L. Adamo [and others] -- Use of affinity chromatography in purification of A1 adenosine receptors from rat brain membranes / Hiroyasu Nakata -- Purification and structure of L-type calcium channels / Toni Schneider [and others] -- Purification and reconstitution of the ryanodine-sensitive Ca2 release channel complex from muscle sarcoplasmic reticulum / F. Anthony Lai and Gerhard Meisner -- Identification of a lignad-gated ion channel by photoaffinity labeling and microsequencing / Ferdinand Hucho -- Voltage-gated ion channels: electrophysiological approaches / Nicholas B. Standen and Peter R. Stanfield -- Electrophysiological approach to the regulation of neuronal voltage-activated calcium channels by guanine nucleotide binding proteins / Roderick H. Scott and Annette C. Dolphin
Control code
260242060
Dimensions
unknown
Extent
1 online resource (xvii, 373 pages)
File format
one file format
Form of item
online
Isbn
9780896031999
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896031993.
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)260242060
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Protocols in molecular neurobiology, edited by Alan Longstaff, Patricia Revest
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Separation of large DNA molecules by pulsed-field gel electrophoresis / Duncan J. Shaw -- Isolation of genomic DNA from invertebrates / Erno Vreugdenhil and Mark G. Darlison -- Analysis of mitochondrial DNA mutations / Masashi Tanaka and Takayuki Ozawa -- Design and use of oligonucleotides / Alan N. Bateson and Mark G. Darlison -- Use of degenerate oligonucleotides for polymerase chain-reaction-based isolation of related DNA sequences / Tania E. Webb and Alan N. Bateson -- Isolation of photoreceptor cell-specific MEKA cDNA by differential hybridization / Che-Hui Kuo -- Generation of isoform-specific antisera from cloned cDNAs: application to multiple forms of the Na/K-ATPase expressed in rat brain / Andrew W. Shyjan and Robert Levenson -- Determination of transcriptional intitiation sites and their usage in the nervous system / Gary Weisinger, Joseph D. DeCristofaro and Edmund F. La Gamma -- Mapping of trans-acting factor binding in the nervous system / Gary Weisinger and Edmund F. La Gamma -- In situ hybridization to brain tissue sections using labeled single-strand complementary RNA probes / Giulio M. Pasinetti -- In situ hybridization histochemistry using alkaline phosphatase-labeled oligodeoxynucleotide probe / Hiroshi Kiyama, Piers C. Emson and Masaya Tohyama -- Identification of neuropeptide gene regulatory elements in transgenic mice / Malcolm J. Low -- Expression of exogenous ion channels and neurotransmitter receptors in RNA-injected Xenopus oocytes / Nathan Dascal and Ilana Lotan -- Analysis of insulin and insulin-like growth factor-I receptors in neural tissues / Martin L. Adamo [and others] -- Use of affinity chromatography in purification of A1 adenosine receptors from rat brain membranes / Hiroyasu Nakata -- Purification and structure of L-type calcium channels / Toni Schneider [and others] -- Purification and reconstitution of the ryanodine-sensitive Ca2 release channel complex from muscle sarcoplasmic reticulum / F. Anthony Lai and Gerhard Meisner -- Identification of a lignad-gated ion channel by photoaffinity labeling and microsequencing / Ferdinand Hucho -- Voltage-gated ion channels: electrophysiological approaches / Nicholas B. Standen and Peter R. Stanfield -- Electrophysiological approach to the regulation of neuronal voltage-activated calcium channels by guanine nucleotide binding proteins / Roderick H. Scott and Annette C. Dolphin
Control code
260242060
Dimensions
unknown
Extent
1 online resource (xvii, 373 pages)
File format
one file format
Form of item
online
Isbn
9780896031999
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/0896031993.
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)260242060
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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