Coverart for item
The Resource RNA silencing : methods and protocols, edited by Gordon Carmichael

RNA silencing : methods and protocols, edited by Gordon Carmichael

Label
RNA silencing : methods and protocols
Title
RNA silencing
Title remainder
methods and protocols
Statement of responsibility
edited by Gordon Carmichael
Contributor
Subject
Genre
Language
eng
Summary
The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications
Member of
Cataloging source
COO
Dewey number
572.8/65
Illustrations
illustrations
Index
index present
LC call number
QP623.5.S63
LC item number
R634 2005
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 309
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 58.7
NLM item number
R6273 2005
http://library.link/vocab/relatedWorkOrContributorName
Carmichael, Gordon
Series statement
Methods in molecular biology
Series volume
309
http://library.link/vocab/subjectName
  • Small interfering RNA
  • Gene silencing
  • Gene Expression Regulation
  • MicroRNAs
  • RNA, Small Interfering
  • RNA Interference
  • MicroRNAs
  • Physiology
  • RNA, Small Interfering
  • Genetics
  • Gene Expression Regulation
  • Genetic Phenomena
  • Biological Science Disciplines
  • Gene Silencing
  • Biology
  • RNA, Small Untranslated
  • RNA, Antisense
  • RNA
  • RNA, Untranslated
  • Antisense Elements (Genetics)
  • Epigenesis, Genetic
  • Natural Science Disciplines
  • Phenomena and Processes
  • Nucleic Acids, Nucleotides, and Nucleosides
  • Nucleic Acids
  • Disciplines and Occupations
  • SCIENCE
  • Gene silencing
  • Small interfering RNA
  • Rna
Label
RNA silencing : methods and protocols, edited by Gordon Carmichael
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of drosophila RNA silencing complexes by native gel electrophoresis / John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in Caenorhabditis elegans / Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei / Christine E. Clayton [and others] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing / M. Florian Mette [and others] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sebastiona Wulchli -- Silencing gene expression with dicer-generated siRNA pools / Jason W. Myers and James E. Ferrell, Jr
  • Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector-based RNAi technology / Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo / Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni [and others] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and Jo Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs / Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elsa Maniataki, Maria Dels Angels de Planell Saguer, and Zissimos Mourelatos
  • Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou [and others] -- High throughput analysis of microRNA gene expression using sensitive cDNA probes / Mouldy Sioud and Øystein Røsok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu [and others]
Control code
209852817
Dimensions
unknown
Extent
1 online resource (xi, 338 pages)
Form of item
online
Isbn
9781588294364
Lccn
2004023107
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
http://library.link/vocab/ext/overdrive/overdriveId
978-1-58829-436-4
Specific material designation
remote
System control number
(OCoLC)209852817
Label
RNA silencing : methods and protocols, edited by Gordon Carmichael
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of drosophila RNA silencing complexes by native gel electrophoresis / John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in Caenorhabditis elegans / Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei / Christine E. Clayton [and others] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing / M. Florian Mette [and others] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sebastiona Wulchli -- Silencing gene expression with dicer-generated siRNA pools / Jason W. Myers and James E. Ferrell, Jr
  • Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector-based RNAi technology / Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo / Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni [and others] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and Jo Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs / Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elsa Maniataki, Maria Dels Angels de Planell Saguer, and Zissimos Mourelatos
  • Detection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou [and others] -- High throughput analysis of microRNA gene expression using sensitive cDNA probes / Mouldy Sioud and Øystein Røsok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu [and others]
Control code
209852817
Dimensions
unknown
Extent
1 online resource (xi, 338 pages)
Form of item
online
Isbn
9781588294364
Lccn
2004023107
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
http://library.link/vocab/ext/overdrive/overdriveId
978-1-58829-436-4
Specific material designation
remote
System control number
(OCoLC)209852817

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...