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The Resource Recombinant gene expression : reviews and protocols, edited by Paulina Balbás and Argelia Lorence

Recombinant gene expression : reviews and protocols, edited by Paulina Balbás and Argelia Lorence

Label
Recombinant gene expression : reviews and protocols
Title
Recombinant gene expression
Title remainder
reviews and protocols
Statement of responsibility
edited by Paulina Balbás and Argelia Lorence
Contributor
Subject
Genre
Language
eng
Summary
In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. The readily reproducible protocols offer new host-vector systems, such as Antarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. There are also novel heterologous gene expression schemes for cell-free systems, plant chloroplasts, microplates, hairy roots, and animal milk, and new developments in consolidated heterologous systems. The protocols follow the successful Methods in Molecular Biology{u2122} series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression
Member of
Cataloging source
COO
http://bibfra.me/vocab/lite/collectionName
Recombinant gene expression protocols
Dewey number
572.8/77
Illustrations
illustrations
Index
index present
LC call number
QH443
LC item number
.R36 2004
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 443
NLM item number
  • ME9616J v.267 2004
  • R3109 2004
http://library.link/vocab/relatedWorkOrContributorName
  • Balbás, Paulina
  • Lorence, Argelia
Series statement
Methods in molecular biology
Series volume
267
http://library.link/vocab/subjectName
  • Genetic recombination
  • Gene Expression
  • Recombination, Genetic
  • SCIENCE
  • Genetic recombination
Label
Recombinant gene expression : reviews and protocols, edited by Paulina Balbás and Argelia Lorence
Instantiates
Publication
Note
Revised edition of: Recombinant gene expression protocols / edited by Rocky S. Tuan. c1997
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Host cell compatibility in protein expression / James J. Greene -- Production of recombinant proteins / Laura A. Palomares, Sandino Estrada-Mondaca, and Octavio T. Ramírez -- Folding promoting agents in recombinant protein production / Beatrix Fahnert -- Back to basics / Paulina Balbás and Francisco Bolivar -- Alpha-complementation enabled t7 expression vectors and their use for the expression of recombinant polypeptides for protein transduction experiments / Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson -- Expression of recombinant alkaline phosphatase conjugates in Escherichia coli / Jean-Claude Boulain and Frédéric Ducancel -- Overexpression of chromosomal genes in Escherichia coli / Fernando Valle and Noemí Flores -- Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli / Lu Zhou, Ke Zhang and Barry L. Wanner
  • Plasmid vectors for marker-free chromosomal insertion of genetic material in Escherichia coli / Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset -- Copy-control pBAC/oriV vectors for genomic cloning / Jadwiga Wild and Waclaw Szybalski -- Copy-control tightly regulated expression vectors based on pBAC/oriV / Jadwiga Wild and Waclaw Szybalski -- Cell-free protein synthesis with prokaryotic combined transcription-translation / James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mumford -- Genetic tools for the manipulation of moderately halophilic bacteria of the family halomonadaceae / Carmen Vargas and Joaquín J. Nieto -- Gene transfer and expression of recombinant proteins in moderately halophilic bacteria / Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto and Constantin Drainas -- Recombinant protein production in antarctic gram-negative bacteria / Angela Duilio, Maria Luisa Tutino, and Gennaro Marino -- Recombinant protein production in yeasts / Danilo Porro and Diethard Mattanovich -- Controlled expression of homologous genes by genomic promoter replacement in the yeast Saccharomyces cerevisiae / Kevin J. Vestrepen and Johan M. Thevelein -- High-throughput expression in microplate format in saccharomyces cerevisiae / Caterina Holz and Christine Lang -- High-throughput expression in microplate format in Pichia pastoris / Mewes Bötner and Christine Lang -- Multiple gene expression by chromosomal integration and CRE-lox mediated marker recycling in saccharomyces cerevisiae / Björn Johansson and Bärbel Hahn-Hägerdal
  • Three decades of fungal transformation / Vianey Olmedo-Monfil, Carlos Cortés-Penagos, and Alfredo Herrera-Estrella -- Three decades of fungal transformation / Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella -- Gene transfer and expression in plants / Argelia Lorence and Robert Verpoorte -- Production of recombinant proteins by hairy roots cultured in plastic sleeve bioreactors / Fabricio Medina-Bolívar and Carole Cramer -- Engineering the chloroplast genome for hyperexpression of human therapeutic proteins and vaccine antigens / Shashi Kumar and Henry Daniell -- New selection marker for plant transformation / Barbara Leyman, Nelson Avonce, Matthew Ramon, Patrick Van Dijck, Johan M. Thevelein, and Gabriel Iturriaga -- Enhancer detection and gene trapping a functional genomics in plants / Gerardo Acosta-García, Daphné Autran, and Jean-Philippe Vielle-Calzada -- Gene transfer and expression in mammalian cell lines and transgenic animals / Felix Recillas-Targa -- Sustained heterologous transgene expression in mammalian and avian cell lines / Héctor Rincón-Arano and Felix Recillas-Targa -- Inducible gene expression in mammalian cells and mice / Wilfried Webber and Martin Fussenegger -- Flp-mediated integration of expression cassettes into FRT-tagged chromosomal lociin mammalian cells / Dagmar Wirth and Hansjörg Hauser -- Generation of high recombinant protein producing Chinese hamster ovary cells / Jean-Louis Goergen and Lucía Monaco -- Preparation of recombinant proteins in milk / Louis-Marie Houdebine
Control code
55684310
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xvi, 506 pages)
Form of item
online
Isbn
9781592597741
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)55684310
Label
Recombinant gene expression : reviews and protocols, edited by Paulina Balbás and Argelia Lorence
Publication
Note
Revised edition of: Recombinant gene expression protocols / edited by Rocky S. Tuan. c1997
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Host cell compatibility in protein expression / James J. Greene -- Production of recombinant proteins / Laura A. Palomares, Sandino Estrada-Mondaca, and Octavio T. Ramírez -- Folding promoting agents in recombinant protein production / Beatrix Fahnert -- Back to basics / Paulina Balbás and Francisco Bolivar -- Alpha-complementation enabled t7 expression vectors and their use for the expression of recombinant polypeptides for protein transduction experiments / Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson -- Expression of recombinant alkaline phosphatase conjugates in Escherichia coli / Jean-Claude Boulain and Frédéric Ducancel -- Overexpression of chromosomal genes in Escherichia coli / Fernando Valle and Noemí Flores -- Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli / Lu Zhou, Ke Zhang and Barry L. Wanner
  • Plasmid vectors for marker-free chromosomal insertion of genetic material in Escherichia coli / Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset -- Copy-control pBAC/oriV vectors for genomic cloning / Jadwiga Wild and Waclaw Szybalski -- Copy-control tightly regulated expression vectors based on pBAC/oriV / Jadwiga Wild and Waclaw Szybalski -- Cell-free protein synthesis with prokaryotic combined transcription-translation / James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mumford -- Genetic tools for the manipulation of moderately halophilic bacteria of the family halomonadaceae / Carmen Vargas and Joaquín J. Nieto -- Gene transfer and expression of recombinant proteins in moderately halophilic bacteria / Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto and Constantin Drainas -- Recombinant protein production in antarctic gram-negative bacteria / Angela Duilio, Maria Luisa Tutino, and Gennaro Marino -- Recombinant protein production in yeasts / Danilo Porro and Diethard Mattanovich -- Controlled expression of homologous genes by genomic promoter replacement in the yeast Saccharomyces cerevisiae / Kevin J. Vestrepen and Johan M. Thevelein -- High-throughput expression in microplate format in saccharomyces cerevisiae / Caterina Holz and Christine Lang -- High-throughput expression in microplate format in Pichia pastoris / Mewes Bötner and Christine Lang -- Multiple gene expression by chromosomal integration and CRE-lox mediated marker recycling in saccharomyces cerevisiae / Björn Johansson and Bärbel Hahn-Hägerdal
  • Three decades of fungal transformation / Vianey Olmedo-Monfil, Carlos Cortés-Penagos, and Alfredo Herrera-Estrella -- Three decades of fungal transformation / Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella -- Gene transfer and expression in plants / Argelia Lorence and Robert Verpoorte -- Production of recombinant proteins by hairy roots cultured in plastic sleeve bioreactors / Fabricio Medina-Bolívar and Carole Cramer -- Engineering the chloroplast genome for hyperexpression of human therapeutic proteins and vaccine antigens / Shashi Kumar and Henry Daniell -- New selection marker for plant transformation / Barbara Leyman, Nelson Avonce, Matthew Ramon, Patrick Van Dijck, Johan M. Thevelein, and Gabriel Iturriaga -- Enhancer detection and gene trapping a functional genomics in plants / Gerardo Acosta-García, Daphné Autran, and Jean-Philippe Vielle-Calzada -- Gene transfer and expression in mammalian cell lines and transgenic animals / Felix Recillas-Targa -- Sustained heterologous transgene expression in mammalian and avian cell lines / Héctor Rincón-Arano and Felix Recillas-Targa -- Inducible gene expression in mammalian cells and mice / Wilfried Webber and Martin Fussenegger -- Flp-mediated integration of expression cassettes into FRT-tagged chromosomal lociin mammalian cells / Dagmar Wirth and Hansjörg Hauser -- Generation of high recombinant protein producing Chinese hamster ovary cells / Jean-Louis Goergen and Lucía Monaco -- Preparation of recombinant proteins in milk / Louis-Marie Houdebine
Control code
55684310
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xvi, 506 pages)
Form of item
online
Isbn
9781592597741
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
(OCoLC)55684310

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