Coverart for item
The Resource Recombinant gene expression protocols, edited by Rocky S. Tuan

Recombinant gene expression protocols, edited by Rocky S. Tuan

Label
Recombinant gene expression protocols
Title
Recombinant gene expression protocols
Statement of responsibility
edited by Rocky S. Tuan
Contributor
Subject
Genre
Language
eng
Summary
Annotation
Member of
Cataloging source
YNG
Dewey number
572.8/77
Illustrations
illustrations
Index
index present
LC call number
QH443
LC item number
.R36 1997
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 443
NLM item number
  • ME9616J v.62 1997
  • R3108 1997
http://library.link/vocab/relatedWorkOrContributorName
Tuan, Rocky S
Series statement
Methods in molecular biology
Series volume
v. 62
http://library.link/vocab/subjectName
  • Genetic recombination
  • Recombination, Genetic
  • Gene Expression
  • Genetic Engineering
  • Genetic recombination
Summary expansion
Expert international research workers offer the most up-to-date and comprehensive collection of techniques available for expressing one or more genes of interest. The authoritative contributors describe in depth the vectors used for expression in bacteria, yeast, viruses, nonmammalian higher eukaryotes, mammals, and plants, as well as in transgenic animals. They also provide invaluable detailed information on how to introduce the recombinant gene construct into the appropriate expression host. When combined with a companion volume by the same editor, Recombinant Proteins: Detection and Isolation Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression
Target audience
adult
Label
Recombinant gene expression protocols, edited by Rocky S. Tuan
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Recombinant gene expression protocols : overview of experimental strategies for the expression of recombinant genes / Rocky S. Tuan -- Designing expression plasmid vectors in E. coli / Paulina Balbás -- Design of bacterial hosts for lac-based expression vectors / Herbert P. Schweizer, RoxAnn R. Karkhoff-Schweizer -- Bacteriophage [lambda]-based expression vectors / Alan C. Christensen -- Strategies for gene fusions / Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén -- Application of the E. coli trp promoter / Daniel G. Yansura, Steven H. Bass -- Expression of proteins in E. coli utilizing a dual promoter-based vector : pLACT7 / George A. Garcia, Shaorong Chong -- Expression and secretion of proteins in E. coli / Ophry Pines, Masayori Inouye -- Synthetic two-cistron expression system / Brigitte E. Schoner -- Expression of cDNA-encoded proteins by cell-free transcription and translation / Chistopher Coleclough -- Yeast plasmids / Keshav K. Singh, Jack A. Heinemann -- Inducible expression cassettes in yeast. GAL4 / Lawrence M. Mylin, James E. Hopper -- Inducible expression cassettes in yeast. ADH2 / Virginia L. Price -- Constitutive expression vectors : PGK / Ian R. Graham, Alistair Chambers -- Design and construction of recombinant vaccinia viruses / Christopher C. Broder, Patricia L. Earl -- A simplified method of screening for isolation of recombinant vaccinia virus / Haifeng Chen, R. Padmanabhan -- Design of retroviral expression vectors / Kenine E. Comstock, Nathaniel F. Watson, John C. Olsen -- Use of defective herpes-derived plasmid vectors / Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu, Yaming Wang, Alfred I. Geller -- Use of baculovirus expression vectors / David R. O'Reilly -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos / Koichiro Shiokawa, Chie Koga, Yuzuru Ito, Mikihito Shibata -- Analysis of heterologous gene expression in Xenopus blastomeres / Sally A. Moody -- Overview of vector design for mammalian gene expression / Randal J. Kaufman -- Experimental strategies in efficient transfection of mammalian cells. Calcium phosphate and DEAE-Dextran / Gregory S. Pari, Wayne A. Keown -- Experimental strategies in efficient transfection of mammalian cells. Electroporation / Donald C. Chang -- Highly effective delivery of foreign DNA to adherent cells via Polybrene/DMSO-assisted gene transfer / Rémy A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, Malcolm C. Paterson -- Selection of transfected cells : magnetic affinity cell sorting / Raji Padmanabhan, Snorri S. Thorgeirsson, R. Padmanabhan -- Selection of transfected cells and coamplification of transfected genes / Susan E. Kane -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells / Jennie P. Mather, Alison Moore, Robert Shawley -- Promoters to express cloned genes uniformly in Drosophila / Jean-Paul Vincent, Charles Girdham -- Application of micromechanical piercing structures for genetic transformation of nematodes / Sarwar Hashmi, Randy Gaugler -- Strategies in generating transgenic mammals / Olena Jacenko -- Use of gonadal primordial germ cells (PGCs) as tools for gene transfer in chickens / Nathalie Allioli, Gérard Verdier, Catherine Legras -- Strategies for the production of transgenic chickens / Robert J. Etches, Ann M. Verrinder Gibbins -- Plant cell transfection by electroporation / William B. Terzaghi, Anthony R. Cashmore -- Transformation and foreign gene expression in plants mediated by microprojectile bombardment / Henry Daniell -- Transformation of the cereals using agrobacterium / Jean Gould -- Transformation of microalgae using silicon carbide whiskers / Terri G. Dunahay, Sally A. Adler, Jonathan W. Jarvik
Control code
260242099
Dimensions
unknown
Extent
1 online resource (xx, 521 pages)
File format
one file format
Form of item
online
Isbn
9781592595488
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote
System control number
(OCoLC)260242099
Label
Recombinant gene expression protocols, edited by Rocky S. Tuan
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Recombinant gene expression protocols : overview of experimental strategies for the expression of recombinant genes / Rocky S. Tuan -- Designing expression plasmid vectors in E. coli / Paulina Balbás -- Design of bacterial hosts for lac-based expression vectors / Herbert P. Schweizer, RoxAnn R. Karkhoff-Schweizer -- Bacteriophage [lambda]-based expression vectors / Alan C. Christensen -- Strategies for gene fusions / Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén -- Application of the E. coli trp promoter / Daniel G. Yansura, Steven H. Bass -- Expression of proteins in E. coli utilizing a dual promoter-based vector : pLACT7 / George A. Garcia, Shaorong Chong -- Expression and secretion of proteins in E. coli / Ophry Pines, Masayori Inouye -- Synthetic two-cistron expression system / Brigitte E. Schoner -- Expression of cDNA-encoded proteins by cell-free transcription and translation / Chistopher Coleclough -- Yeast plasmids / Keshav K. Singh, Jack A. Heinemann -- Inducible expression cassettes in yeast. GAL4 / Lawrence M. Mylin, James E. Hopper -- Inducible expression cassettes in yeast. ADH2 / Virginia L. Price -- Constitutive expression vectors : PGK / Ian R. Graham, Alistair Chambers -- Design and construction of recombinant vaccinia viruses / Christopher C. Broder, Patricia L. Earl -- A simplified method of screening for isolation of recombinant vaccinia virus / Haifeng Chen, R. Padmanabhan -- Design of retroviral expression vectors / Kenine E. Comstock, Nathaniel F. Watson, John C. Olsen -- Use of defective herpes-derived plasmid vectors / Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu, Yaming Wang, Alfred I. Geller -- Use of baculovirus expression vectors / David R. O'Reilly -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos / Koichiro Shiokawa, Chie Koga, Yuzuru Ito, Mikihito Shibata -- Analysis of heterologous gene expression in Xenopus blastomeres / Sally A. Moody -- Overview of vector design for mammalian gene expression / Randal J. Kaufman -- Experimental strategies in efficient transfection of mammalian cells. Calcium phosphate and DEAE-Dextran / Gregory S. Pari, Wayne A. Keown -- Experimental strategies in efficient transfection of mammalian cells. Electroporation / Donald C. Chang -- Highly effective delivery of foreign DNA to adherent cells via Polybrene/DMSO-assisted gene transfer / Rémy A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, Malcolm C. Paterson -- Selection of transfected cells : magnetic affinity cell sorting / Raji Padmanabhan, Snorri S. Thorgeirsson, R. Padmanabhan -- Selection of transfected cells and coamplification of transfected genes / Susan E. Kane -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells / Jennie P. Mather, Alison Moore, Robert Shawley -- Promoters to express cloned genes uniformly in Drosophila / Jean-Paul Vincent, Charles Girdham -- Application of micromechanical piercing structures for genetic transformation of nematodes / Sarwar Hashmi, Randy Gaugler -- Strategies in generating transgenic mammals / Olena Jacenko -- Use of gonadal primordial germ cells (PGCs) as tools for gene transfer in chickens / Nathalie Allioli, Gérard Verdier, Catherine Legras -- Strategies for the production of transgenic chickens / Robert J. Etches, Ann M. Verrinder Gibbins -- Plant cell transfection by electroporation / William B. Terzaghi, Anthony R. Cashmore -- Transformation and foreign gene expression in plants mediated by microprojectile bombardment / Henry Daniell -- Transformation of the cereals using agrobacterium / Jean Gould -- Transformation of microalgae using silicon carbide whiskers / Terri G. Dunahay, Sally A. Adler, Jonathan W. Jarvik
Control code
260242099
Dimensions
unknown
Extent
1 online resource (xx, 521 pages)
File format
one file format
Form of item
online
Isbn
9781592595488
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote
System control number
(OCoLC)260242099

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