The Resource Role of RNase activity in interspecific pollen rejection in Nicotiana, by Brian Stuart Beecher

Role of RNase activity in interspecific pollen rejection in Nicotiana, by Brian Stuart Beecher

Label
Role of RNase activity in interspecific pollen rejection in Nicotiana
Title
Role of RNase activity in interspecific pollen rejection in Nicotiana
Statement of responsibility
by Brian Stuart Beecher
Title variation
RNases and interspecific pollen rejection
Creator
Subject
Language
eng
Summary
Unilateral incompatibility (UI) often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. The SI species Nicotiana alata rejects pollen from its SC relatives N. plumbaginifolia , and N. tabacum , but the reciprocal pollinations are compatible. These interspecific pollen rejection systems resemble intraspecific S-allele-specific pollen rejection in that they are S-RNase dependent. However, these two interspecific UI systems and the intra-specific SI system display different degrees of specificity. SI is extremely specific, a single S-RNase causes rejection of only one pollen S-genotype while the UI systems show broader specificity. In this dissertation, I have used plant transformation to better define the specificity of these two UI systems. The approach was to express different ribonucleases in defined genetic backgrounds and then test for changes in compatibility with N. plumbaginifolia and N. tabacum pollen. Three RNases with different degrees of similarity to wild-type S-RNase sequence were chosen. S 9811 -RNase is an S-RNase that is functional in SI, but apparently not functional in rejection of N. Plumbaginifolia pollen. Con5-RNase is a chimera of two wild-type S-RNases that does not function in SI. RNaseI from E. coli is a non-S-RNase. These test RNases were compared with controls expressing wild type S-RNase (S A2 - or S C10 -RNase) at levels sufficient for pollen rejection. When expressed to high levels in transgenic plants, recombinant S 9811 -RNase caused rejection of N. tabacum pollen. However it failed to cause similar rejection of N. Plumbaginifolia pollen, even when expressed at 2-fold higher levels than control N. plumbaginifolia pollen-rejecting plants expressing wild type SA2-RNase. Similar results were obtained for expression of Con5-RNase. However, RNaseI from E. coli did not cause rejection of either N. plumbaginifolia or N. tabacum pollen, demonstrating that RNase activity per se is not sufficient. Together, these results suggest that S-RNases are specially adapted for their pollen rejection functions. These results also suggest that different degrees of adaptation are required in the different pollen rejection systems
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1970-
http://library.link/vocab/creatorName
Beecher, Brian Stuart
Degree
Ph. D.
Dissertation year
1999.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
bibliography
http://library.link/vocab/subjectName
  • Plants
  • Pollination
  • Ribonucleases
  • Fertilization of plants
  • Nicotiana
Target audience
specialized
Label
Role of RNase activity in interspecific pollen rejection in Nicotiana, by Brian Stuart Beecher
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 246-266)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
46886401
Dimensions
29 cm
Dimensions
unknown
Extent
ix, 267 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
Label
Role of RNase activity in interspecific pollen rejection in Nicotiana, by Brian Stuart Beecher
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 246-266)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
46886401
Dimensions
29 cm
Dimensions
unknown
Extent
ix, 267 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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