The Resource Single cell assays of exocytosis, by Peng Chen

Single cell assays of exocytosis, by Peng Chen

Label
Single cell assays of exocytosis
Title
Single cell assays of exocytosis
Statement of responsibility
by Peng Chen
Creator
Subject
Language
eng
Summary
Cells package signaling molecules (e.g. catecholamine and neurotransmitter) within membrane-encased vesicles. Upon cell stimulation, a rise in intracellular Ca [superscript 2+] concentration triggers the fusion of vesicles with the cell's outer membrane and release of contents into extracellular space in a process called exocytosis. Understanding the process whereby cells transduce an external signal to a secretory response has been an important topic of research of many years. This thesis aims at improving current techniques and developing new techniques for single cell assays of exocytosis. Membrane capacitance measurements of the voltage-clamped cell membrane have revolutionized the studies of exocytosis with unprecedented resolution, because changes in membrane surface area accompany fusion of secretory vesicles with the plasma membrane. Our noise study predicts the theoretical limits of capacitance measurements in the whole-cell configuration, and provides guidelines to choose optimal experimental conditions. Membrane conductance changes often occur concurrently with the stimulus used to evoke exocytosis and can lead to unreliable estimation of membrane capacitance. To measure membrane capacitance faithfully even in presence of large linear (e.g. CFTR current) or non-linear (Ca [superscript 2+] current) ionic currents, we have developed a new cell model to describe "non-ideal" membrane properties and new approaches for estimating membrane capacitance. Electrochemical detection of secretion with micro-carbon fiber microelectrodes has been used as a complementary approach to capacitance measurement to study exocytosis. However, carbon fibers can only capture 2%-15% of total cell secretion due to its planar geometry. We have fabricated picoliter-sized electrochemical electrodes/wells on silicon chips for measuring catecholamine release from individual cells with millisecond resolution. Each well-electrode roughly conforms the shape of the cell in order to capture a large fraction of released catecholamine. By applying engineering principles and techniques for biological studies, this work should advance the electrophysiological assays of exocytosis and lead to a better understanding of its mechanism and regulation
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1971-
http://library.link/vocab/creatorName
Chen, Peng
Degree
Ph. D.
Dissertation year
2002.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Exocytosis
  • Electrochemical sensors
  • Cellular signal transduction
  • Cell interaction
Target audience
specialized
Label
Single cell assays of exocytosis, by Peng Chen
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 149-157)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
52491139
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 158 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color)
Specific material designation
remote
Label
Single cell assays of exocytosis, by Peng Chen
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 149-157)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
52491139
Dimensions
29 cm
Dimensions
unknown
Extent
xi, 158 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations (some color)
Specific material designation
remote

Library Locations

    • Engineering Library & Technology CommonsBorrow it
      W2001 Lafferre Hall, Columbia, MO, 65211, US
      38.946102 -92.330125
    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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