The Resource Spectroscopic and chromatographic study of selective fluorescence quenchers of polycylcic aromatic hydrocarbons (PAHS), by Chunfeng Mao

Spectroscopic and chromatographic study of selective fluorescence quenchers of polycylcic aromatic hydrocarbons (PAHS), by Chunfeng Mao

Label
Spectroscopic and chromatographic study of selective fluorescence quenchers of polycylcic aromatic hydrocarbons (PAHS)
Title
Spectroscopic and chromatographic study of selective fluorescence quenchers of polycylcic aromatic hydrocarbons (PAHS)
Statement of responsibility
by Chunfeng Mao
Creator
Subject
Language
eng
Summary
The ability of pyridinium chloride (PC) and diisopropylamine to selectively quench the fluorescence of one class of polycyclic aromatic hydrocarbons (PAHs) as opposed to the other class was investigated spectroscopically. Pyridinium chloride was identified as selective fluorescence-quenching agent for alternant PAHs and diisopropylamine for nonalternant PAHs. The first uses of identified PC, cetylpyridinium chloride (CPC), and dodecylpyridinium chloride (DDPC), as selective fluorescence-quenching agents of alternant PAHs, under actual HPLC separation conditions were studied. All three quenchers are superior to nitromethane, the only reported PAH selective quencher used in HPLC. Both CPC and DDPC are surfactants and selective fluorescence-quenching agents. Therefore, their dual role--as micellar mobile phase modifier and selective quencher--was studied. It was found that the quenching selectivity of PC, CPC, and DDPC was retained under actual separation conditions, and the addition of a small amount of quencher substantially simplified the fluorescence-detected chromatograms, facilitating PAH identification. Also studied were the optimizations of the micellar liquid chromatographic (MLC) separation of PAHs with three micellar systems, cetyltrimethylammonium chloride (CTAC), dodecyltrimethylammonium chloride (DTAC), and sodium dodecylsulfate (SDS). The optimizations were first conducted with pentanol as the only organic additive. Under optimized separation conditions, CTAC-modified mobile phase provided the least desirable separation, which was attributed to its four-carbon longer tail. The 3% addition of propanol, a second organic additive, in addition to the main organic additive, pentanol, in the micelle-modified mobile phase enhanced the resolution of PAH chromatographic peaks. However, the extent of the enhancement varied for different micellar systems. This study showed the potential use of a second, less nonpolar, organic additive in facilitating the MLC separation of nonpolar compounds
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1974-
http://library.link/vocab/creatorName
Mao, Chunfeng
Degree
Ph. D.
Dissertation year
2003.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Polycyclic aromatic hydrocarbons
  • Fluorescence
  • High performance liquid chromatography
Target audience
specialized
Label
Spectroscopic and chromatographic study of selective fluorescence quenchers of polycylcic aromatic hydrocarbons (PAHS), by Chunfeng Mao
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 101-107)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
54052490
Dimensions
29 cm
Extent
xi, 114 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote
Label
Spectroscopic and chromatographic study of selective fluorescence quenchers of polycylcic aromatic hydrocarbons (PAHS), by Chunfeng Mao
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 101-107)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
54052490
Dimensions
29 cm
Extent
xi, 114 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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