Coverart for item
The Resource Spliceosomal pre-mRNA splicing : methods and protocols, edited by Klemens J. Hertel

Spliceosomal pre-mRNA splicing : methods and protocols, edited by Klemens J. Hertel

Label
Spliceosomal pre-mRNA splicing : methods and protocols
Title
Spliceosomal pre-mRNA splicing
Title remainder
methods and protocols
Statement of responsibility
edited by Klemens J. Hertel
Contributor
Editor
Subject
Genre
Language
eng
Summary
Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo, manipulation of splicing events, and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come
Member of
Cataloging source
KIJ
Dewey number
  • 660.6/5
  • 611.01816
  • 599.935
Illustrations
illustrations
Image bit depth
0
Index
index present
Language note
English
LC call number
QH450
LC item number
.S65 2014
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.1126 2014
http://library.link/vocab/relatedWorkOrContributorName
Hertel, Klemens J.
Series statement
Methods in Molecular Biology, Methods and Protocols,
Series volume
1126
http://library.link/vocab/subjectName
  • RNA splicing
  • Genetic engineering
  • RNA
  • RNA Processing, Post-Transcriptional
  • Nucleic Acid Precursors
  • Intranuclear Space
  • Nucleic Acids
  • Cell Nucleus Structures
  • Biochemical Phenomena
  • Metabolism
  • Gene Expression Regulation
  • Nucleic Acids, Nucleotides, and Nucleosides
  • Cell Nucleus
  • Genetic Phenomena
  • Chemical Phenomena
  • Intracellular Space
  • Phenomena and Processes
  • Cellular Structures
  • Cells
  • Anatomy
  • RNA Precursors
  • RNA Splicing
  • RNA, Messenger
  • Spliceosomes
  • Genetic engineering
  • RNA splicing
Label
Spliceosomal pre-mRNA splicing : methods and protocols, edited by Klemens J. Hertel
Instantiates
Publication
Copyright
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Regulation of alternative pre-mRNA splicing
  • Miguel B. Coelho and Christopher W.J. Smith
  • Introduction to cotranscriptional RNA splicing
  • Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson
  • Chromatin and splicing
  • Nazmul Haque and Shalini Oberdoerffer
  • Preparation of splicing competent nuclear extracts
  • Chiu-Ho T. Webb and Klemens J. Hertel
  • Preparation of yeast whole cell splicing extract
  • Elizabeth A. Dunn and Stephen D. Rader
  • Pre-mRNA splicing reaction
  • Efficient splinted ligation of synthetic RNA using RNA ligase
  • Martha R. Stark and Stephen D. Rader
  • In vitro assay of pre-mRNA splicing in mammalian nuclear extract
  • Maliheh Movassat, William F. Mueller, and Klemens J. Hertel
  • Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract
  • William F. Mueller and Klemens J. Hertel
  • In vitro systems for coupling RNAP II transcription to splicing and polyadenylation
  • Eric G. Folco and Robin Reed
  • Isolation and accumulation of spliceosomal assembly intermediates
  • Janine O. Ilagan and Melissa S. Jurica
  • Somsakul Pop Wongpalee and Shalini Sharma
  • Complementation of U4 snRNA in S. cerevisiae splicing extracts for biochemical studies of snRNP assembly and function
  • Martha R. Stark and Stephen D. Rader
  • Expression and purification of splicing proteins from mammalian cells
  • Eric Allemand and Michelle L. Hastings
  • Single molecule approaches for studying spliceosome assembly and catalysis
  • Eric G. Anderson and Aaron A. Hoskins
  • Cell-based splicing of minigenes
  • Sarah A. Smith and Kristen W. Lynch
  • Quantifying the ratio of spliceosome components assembled on pre-mRNA
  • Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal
  • Diversity and evolution of spliceosomal systems
  • Antisense methods to modulate pre-mRNA splicing
  • Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh
  • Using yeast genetics to study splicing mechanisms
  • Munshi Azad Hossain and Tracy L. Johnson
  • Medium throughput analysis of alternative splicing by fluorescently labeled RT-PCR
  • Ryan Percifield, Daniel Murphy, and Peter Stoilov
  • Chromatin immunoprecipitation approaches to determine co-transcriptional nature of splicing
  • Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer
  • Computational approaches to mine publicly available databases
  • Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund
  • Scott William Roy and Manuel Irimia
  • Approaches to link RNA secondary structures with splicing regulation
  • Mireya Plass and Eduardo Eyras
  • Methods to study splicing from high-throughput RNA sequencing data
  • Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras--
  • Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq
  • Chengguo Yao, Lingjie Weng, and Yongsheng Shi
  • Predicting alternative splicing
  • Yoseph Barash and Jorge Vaquero Garcia
  • Mechanisms of Spliceosomal Assembly
  • Ni-ting Chiou and Kristen W. Lynch
  • Alternative pre-mRNA splicing
  • Stacey D. Wagner and J. Andrew Berglund
Control code
878439645
Dimensions
unknown
Extent
1 online resource (xi, 427 pages)
File format
multiple file formats
Form of item
online
Isbn
9781627039802
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-980-2
Other physical details
illustrations (some color).
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
(OCoLC)878439645
Label
Spliceosomal pre-mRNA splicing : methods and protocols, edited by Klemens J. Hertel
Publication
Copyright
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Regulation of alternative pre-mRNA splicing
  • Miguel B. Coelho and Christopher W.J. Smith
  • Introduction to cotranscriptional RNA splicing
  • Evan C. Merkhofer, Peter Hu, and Tracy L. Johnson
  • Chromatin and splicing
  • Nazmul Haque and Shalini Oberdoerffer
  • Preparation of splicing competent nuclear extracts
  • Chiu-Ho T. Webb and Klemens J. Hertel
  • Preparation of yeast whole cell splicing extract
  • Elizabeth A. Dunn and Stephen D. Rader
  • Pre-mRNA splicing reaction
  • Efficient splinted ligation of synthetic RNA using RNA ligase
  • Martha R. Stark and Stephen D. Rader
  • In vitro assay of pre-mRNA splicing in mammalian nuclear extract
  • Maliheh Movassat, William F. Mueller, and Klemens J. Hertel
  • Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract
  • William F. Mueller and Klemens J. Hertel
  • In vitro systems for coupling RNAP II transcription to splicing and polyadenylation
  • Eric G. Folco and Robin Reed
  • Isolation and accumulation of spliceosomal assembly intermediates
  • Janine O. Ilagan and Melissa S. Jurica
  • Somsakul Pop Wongpalee and Shalini Sharma
  • Complementation of U4 snRNA in S. cerevisiae splicing extracts for biochemical studies of snRNP assembly and function
  • Martha R. Stark and Stephen D. Rader
  • Expression and purification of splicing proteins from mammalian cells
  • Eric Allemand and Michelle L. Hastings
  • Single molecule approaches for studying spliceosome assembly and catalysis
  • Eric G. Anderson and Aaron A. Hoskins
  • Cell-based splicing of minigenes
  • Sarah A. Smith and Kristen W. Lynch
  • Quantifying the ratio of spliceosome components assembled on pre-mRNA
  • Noa Neufeld, Yehuda Brody, and Yaron Shav-Tal
  • Diversity and evolution of spliceosomal systems
  • Antisense methods to modulate pre-mRNA splicing
  • Joonbae Seo, Eric W. Ottesen, and Ravindra N. Singh
  • Using yeast genetics to study splicing mechanisms
  • Munshi Azad Hossain and Tracy L. Johnson
  • Medium throughput analysis of alternative splicing by fluorescently labeled RT-PCR
  • Ryan Percifield, Daniel Murphy, and Peter Stoilov
  • Chromatin immunoprecipitation approaches to determine co-transcriptional nature of splicing
  • Nicole I. Bieberstein, Korinna Straube, and Karla M. Neugebauer
  • Computational approaches to mine publicly available databases
  • Rodger B. Voelker, William A. Cresko, and J. Andrew Berglund
  • Scott William Roy and Manuel Irimia
  • Approaches to link RNA secondary structures with splicing regulation
  • Mireya Plass and Eduardo Eyras
  • Methods to study splicing from high-throughput RNA sequencing data
  • Gael P. Alamancos, Eneritz Agirre, and Eduardo Eyras--
  • Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq
  • Chengguo Yao, Lingjie Weng, and Yongsheng Shi
  • Predicting alternative splicing
  • Yoseph Barash and Jorge Vaquero Garcia
  • Mechanisms of Spliceosomal Assembly
  • Ni-ting Chiou and Kristen W. Lynch
  • Alternative pre-mRNA splicing
  • Stacey D. Wagner and J. Andrew Berglund
Control code
878439645
Dimensions
unknown
Extent
1 online resource (xi, 427 pages)
File format
multiple file formats
Form of item
online
Isbn
9781627039802
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-980-2
Other physical details
illustrations (some color).
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
(OCoLC)878439645

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