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The Resource Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols, edited by Bettina Warscheid

Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols, edited by Bettina Warscheid

Label
Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols
Title
Stable isotope labeling by amino acids in cell culture (SILAC)
Title remainder
methods and protocols
Statement of responsibility
edited by Bettina Warscheid
Contributor
Editor
Subject
Genre
Language
eng
Summary
"Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC): Methods and Protocols provides a synopsis of a large array of different SILAC methods by presenting a set of protocols that have been established by renowned scientists and their working groups. These include methods and protocols for the labeling of various model organisms as well as advanced strategies relying on SILAC, e.g. for the analysis of protein interactions, the mapping of posttranslational modifications or the characterization of subcellular proteomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-bystep, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls."--Publisher's description
Member of
Cataloging source
GW5XE
Dewey number
571.6/38
Illustrations
illustrations
Index
index present
LC call number
  • QP551
  • QH585.2
LC item number
.S738 2014
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.1188 2014
http://library.link/vocab/relatedWorkOrContributorName
Warscheid, Bettina
Series statement
Methods in Molecular Biology,
Series volume
Volume No. 1188
http://library.link/vocab/subjectName
  • Proteomics
  • Stable isotopes
  • Amino acids
  • Cell culture
  • Mass spectrometry
  • Amino Acids
  • Isotope Labeling
  • Cell Culture Techniques
  • Proteomics
  • Stable isotopes
  • Amino acids
  • Cell culture
  • Mass spectrometry
  • Biology
Label
Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols, edited by Bettina Warscheid
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Silke Oeljeklaus, Andreas Schummer, Ida Suppanz, and Bettina Warscheid
  • 4.
  • Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei Using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry
  • Olivera Cirovic and Torsten Ochsenreiter
  • 5.
  • Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons
  • Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert
  • 6.
  • SILAC and Alternatives in Studying Cellular Proteomes of Plants
  • Annemarie Matthes, Karin Köhl, and Waltraud X. Schulze
  • 1.
  • 7.
  • In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster
  • Matthias D. Sury, Jia-Xuan Chen, and Matthias Selbach
  • 8.
  • Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse
  • Soraya Hölper, Aaron Ruhs, and Marcus Krüger
  • 9.
  • Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans
  • Julius Fredens, Kasper Engholm-Keller, Jakob Møller-Jensen, Martin Røssel Larsen, and Nils J. Færgeman
  • 10.
  • Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
  • SILAC-Based Temporal Phosphoproteomics
  • Chiara Francavilla, Omid Hekmat, Blagoy Blagoev, and Jesper V. Olsen
  • 11.
  • Global Ubiquitination Analysis by SILAC in Mammalian Cells
  • Zhiping Wu, Chan Hyun Na, Haiyan Tan, and Junmin Peng
  • 12.
  • Quantifying In Vivo, Site-Specific Changes in Protein Methylation with SILAC
  • Ho-Tak Lau, Karen A. Lewis, and Shao-En Ong
  • 13.
  • Applying SILAC for the Differential Analysis of Protein Complexes
  • Matthias Mann
  • Karsten Boldt, Christian J. Gloeckner, Yves Texier, Felix von Zweydorf, and Marius Ueffing
  • 14.
  • Defining Dynamic Protein Interactions Using SILAC-Based Quantitative Mass Spectrometry
  • Xiaorong Wang and Lan Huang
  • 15.
  • Identifying Nuclear Protein-Protein Interactions Using GFP Affinity Purification and SILAC-Based Quantitative Mass Spectrometry
  • H. Irem Baymaz, Cornelia G. Spruijt, and Michiel Vermeulen
  • 16.
  • Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC
  • Carla Schmidt, Monika Raabe, Reinhard Lührmann, and Henning Urlaub
  • 2.
  • 17.
  • Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling
  • Frederik Sommer, Timo Mühlhaus, Dorothea Hemme, Daniel Veyel, and Michael Schroda
  • 18.
  • Protein Correlation Profiling-SILAC to Study Protein- Protein Interactions
  • Anders R. Kristensen and Leonard J. Foster
  • 19.
  • Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC
  • Andrea C. Becker and Jörn Dengjel
  • 20.
  • Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture
  • Design and Application of Super-SILAC for Proteome Quantification
  • Yair Pozniak and Tamar Geiger
  • 21.
  • Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies
  • Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari, and Tiziana Bonaldi
  • 22.
  • Analysis of Secreted Proteins Using SILAC
  • Jeanette Henningsen, Blagoy Blagoev, and Irina Kratchmarova
  • 23.
  • Identification of MicroRNA Targets by Pulsed SILAC
  • Boumediene Soufi and Boris Macek
  • Markus Kaller, Silke Oeljeklaus, Bettina Warscheid, and Heiko Hermeking
  • 24.
  • MaxQuant for In-Depth Analysis of Large SILAC Datasets
  • Stefka Tyanova, Matthias Mann, and Jürgen Cox
  • 3.
  • SILAC Labeling of Yeast for the Study of Membrane Protein Complexes
Control code
885338637
Dimensions
unknown
Extent
1 online resource (xiv, 372 pages)
File format
unknown
Form of item
online
Isbn
9781493911424
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-1142-4
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)885338637
Label
Stable isotope labeling by amino acids in cell culture (SILAC) : methods and protocols, edited by Bettina Warscheid
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Silke Oeljeklaus, Andreas Schummer, Ida Suppanz, and Bettina Warscheid
  • 4.
  • Whole Proteome Analysis of the Protozoan Parasite Trypanosoma brucei Using Stable Isotope Labeling by Amino Acids in Cell Culture and Mass Spectrometry
  • Olivera Cirovic and Torsten Ochsenreiter
  • 5.
  • Stable Isotope Labeling by Amino Acids in Cultured Primary Neurons
  • Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert
  • 6.
  • SILAC and Alternatives in Studying Cellular Proteomes of Plants
  • Annemarie Matthes, Karin Köhl, and Waltraud X. Schulze
  • 1.
  • 7.
  • In Vivo Stable Isotope Labeling by Amino Acids in Drosophila melanogaster
  • Matthias D. Sury, Jia-Xuan Chen, and Matthias Selbach
  • 8.
  • Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse
  • Soraya Hölper, Aaron Ruhs, and Marcus Krüger
  • 9.
  • Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans
  • Julius Fredens, Kasper Engholm-Keller, Jakob Møller-Jensen, Martin Røssel Larsen, and Nils J. Færgeman
  • 10.
  • Fifteen Years of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
  • SILAC-Based Temporal Phosphoproteomics
  • Chiara Francavilla, Omid Hekmat, Blagoy Blagoev, and Jesper V. Olsen
  • 11.
  • Global Ubiquitination Analysis by SILAC in Mammalian Cells
  • Zhiping Wu, Chan Hyun Na, Haiyan Tan, and Junmin Peng
  • 12.
  • Quantifying In Vivo, Site-Specific Changes in Protein Methylation with SILAC
  • Ho-Tak Lau, Karen A. Lewis, and Shao-En Ong
  • 13.
  • Applying SILAC for the Differential Analysis of Protein Complexes
  • Matthias Mann
  • Karsten Boldt, Christian J. Gloeckner, Yves Texier, Felix von Zweydorf, and Marius Ueffing
  • 14.
  • Defining Dynamic Protein Interactions Using SILAC-Based Quantitative Mass Spectrometry
  • Xiaorong Wang and Lan Huang
  • 15.
  • Identifying Nuclear Protein-Protein Interactions Using GFP Affinity Purification and SILAC-Based Quantitative Mass Spectrometry
  • H. Irem Baymaz, Cornelia G. Spruijt, and Michiel Vermeulen
  • 16.
  • Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC
  • Carla Schmidt, Monika Raabe, Reinhard Lührmann, and Henning Urlaub
  • 2.
  • 17.
  • Identification and Validation of Protein-Protein Interactions by Combining Co-immunoprecipitation, Antigen Competition, and Stable Isotope Labeling
  • Frederik Sommer, Timo Mühlhaus, Dorothea Hemme, Daniel Veyel, and Michael Schroda
  • 18.
  • Protein Correlation Profiling-SILAC to Study Protein- Protein Interactions
  • Anders R. Kristensen and Leonard J. Foster
  • 19.
  • Autophagosomal Proteome Analysis by Protein Correlation Profiling-SILAC
  • Andrea C. Becker and Jörn Dengjel
  • 20.
  • Stable Isotope Labeling by Amino Acids Applied to Bacterial Cell Culture
  • Design and Application of Super-SILAC for Proteome Quantification
  • Yair Pozniak and Tamar Geiger
  • 21.
  • Proteomics Meets Genetics: SILAC Labeling of Drosophila melanogaster Larvae and Cells for In Vivo Functional Studies
  • Alessandro Cuomo, Roberta Sanfilippo, Thomas Vaccari, and Tiziana Bonaldi
  • 22.
  • Analysis of Secreted Proteins Using SILAC
  • Jeanette Henningsen, Blagoy Blagoev, and Irina Kratchmarova
  • 23.
  • Identification of MicroRNA Targets by Pulsed SILAC
  • Boumediene Soufi and Boris Macek
  • Markus Kaller, Silke Oeljeklaus, Bettina Warscheid, and Heiko Hermeking
  • 24.
  • MaxQuant for In-Depth Analysis of Large SILAC Datasets
  • Stefka Tyanova, Matthias Mann, and Jürgen Cox
  • 3.
  • SILAC Labeling of Yeast for the Study of Membrane Protein Complexes
Control code
885338637
Dimensions
unknown
Extent
1 online resource (xiv, 372 pages)
File format
unknown
Form of item
online
Isbn
9781493911424
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-1142-4
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)885338637

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      38.944491 -92.326012
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