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The Resource Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor, by Kai Tian

Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor, by Kai Tian

Label
Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor
Title
Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor
Statement of responsibility
by Kai Tian
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
The nanopore sensor can detect cancer-derived nucleic acid biomarkers such as microRNAs (miRNAs), providing a noninvasive tool potentially useful in medical diagnostics. However, the nanopore-based detection of these biomarkers remains confounded by the presence of numerous other nucleic acid species found in biofluid extracts. Their nonspecific interactions with the nanopore inevitably contaminate the target signals, reducing the detection accuracy. Here we report a novel method that utilizes a polycationic peptide-PNA probe as the carrier for selective nucleic acid detection in the nucleic acids mixture. The cationic probe hybridized with DNA or RNA forms a dipole complex, which can be captured by the pore using a voltage polarity that is opposite the polarity used to capture negatively charged nucleic acids. As a result, non-target species are driven away from the pore opening, and the target sequences can be detected accurately without interference. In addition, we demonstrate that the PNA probe enables to accurately discriminate single-nucleotide difference. Moreover, molecule dynamic simulation is applied to expose the mechanism. Combined with experimental and calculating data, we construct a model to demonstrate that it is universal for all kinds of nucleic acid targets. In sum, this highly sensitive and selective nano-dielectrophoresis approach can be applied to the detection of clinically relevant nucleic acid fragments in complex samples and fulfills the diagnostic of diseases in early stage
Cataloging source
MUU
http://library.link/vocab/creatorDate
1986-
http://library.link/vocab/creatorName
Tian, Kai
Degree
PhD
Dissertation note
Thesis
Dissertation year
2015.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Gu, Li-Qun
http://library.link/vocab/subjectName
  • Dielectrophoresis
  • Nanopores
  • Biosensors
  • Nucleic acids
  • Peptides
  • Cancer
Label
Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor, by Kai Tian
Instantiates
Publication
Note
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri-Columbia In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Liqun Gu
  • Includes vita
Bibliography note
Includes bibliographical references (pages 77-88)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
959888656
Extent
1 online resource (xi, 89 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)959888656
Label
Study of dielectrophoresis effect of dipolar molecules in electric field of nanopore and development of medical diagnostic nanopore sensor, by Kai Tian
Publication
Note
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri-Columbia In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. Liqun Gu
  • Includes vita
Bibliography note
Includes bibliographical references (pages 77-88)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
959888656
Extent
1 online resource (xi, 89 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)959888656

Library Locations

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      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
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