The Resource The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
Resource Information
The item The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established classical assays and recently developed new assays to analyze the complement system activation will be useful to a wide audience of scientists
- Language
- eng
- Extent
- 1 online resource (xvi, 383 pages)
- Contents
-
- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
- Purification and Functional Analysis of Human Properdin
- Purification and Functional Characterization of C4b-binding Protein (C4BP)
- Purification and Functional Characterization of Factor I
- C1 inhibitor? Quantification and Purification
- Purification, Quantification and Functional Analysis of Complement Factor H
- Factor H-related Proteins
- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
- Evaluation of Complement Function by ELISA
- Anti-Factor H Auto Antibodies Assay
- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Functional Analysis of C5a Effector Responses In Vitro and In Vivo
- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
- Purification of C1q Receptors and Functional Analysis
- Detection of Cell Membrane-bound CD46 using Flow Cytometry
- Detection of CD59 using Flow Cytometry
- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
- Classical Complement Pathway Component C1q? Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q? Derivatives. Functional Characterization
- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
- Analysis of the Complement Activation in Mice
- Opsonophagocytic Assay
- Classical Complement Pathway Components C1r and C1s? Purification from Human Serum and in Recombinant Form and Functional Characterization
- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
- Purification and Characterisation of human and Mouse Complement C3
- Purification of Human Complement Protein C5
- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
- Isbn
- 9781627037242
- Label
- The complement system : methods and protocols
- Title
- The complement system
- Title remainder
- methods and protocols
- Statement of responsibility
- edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
- Subject
-
- Amino Acids, Peptides, and Proteins
- Blood Proteins
- Complement (Immunology)
- Complement (Immunology) -- Laboratory manuals
- Complement Activation
- Complement System Proteins
- Immune System Phenomena
- Immunoproteins
- Laboratory Manuals
- Laboratory manuals
- Membrane Proteins
- Phenomena and Processes
- Proteins
- Publication Characteristics
- Publication Formats
- Receptors, Cell Surface
- Receptors, Complement
- Receptors, Immunologic
- Language
- eng
- Summary
- Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established classical assays and recently developed new assays to analyze the complement system activation will be useful to a wide audience of scientists
- Cataloging source
- GW5XE
- Dewey number
- 571.9/688
- Illustrations
- illustrations
- Index
- index present
- Language note
- English
- LC call number
- QR185.8.C6
- Literary form
- non fiction
- Nature of contents
-
- dictionaries
- bibliography
- NLM call number
-
- W1
- QW 525
- NLM item number
- ME9616J v.1100 2014
- http://library.link/vocab/relatedWorkOrContributorName
- Gadjeva, Mihaela
- Series statement
- Methods in Molecular Biology,
- Series volume
- 1100
- http://library.link/vocab/subjectName
-
- Complement (Immunology)
- Immunoproteins
- Publication Formats
- Immune System Phenomena
- Receptors, Immunologic
- Blood Proteins
- Receptors, Cell Surface
- Publication Characteristics
- Membrane Proteins
- Phenomena and Processes
- Proteins
- Amino Acids, Peptides, and Proteins
- Complement System Proteins
- Receptors, Complement
- Laboratory Manuals
- Complement Activation
- Complement (Immunology)
- Label
- The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
- Antecedent source
- unknown
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
- Purification and Functional Analysis of Human Properdin
- Purification and Functional Characterization of C4b-binding Protein (C4BP)
- Purification and Functional Characterization of Factor I
- C1 inhibitor? Quantification and Purification
- Purification, Quantification and Functional Analysis of Complement Factor H
- Factor H-related Proteins
- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
- Evaluation of Complement Function by ELISA
- Anti-Factor H Auto Antibodies Assay
- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Functional Analysis of C5a Effector Responses In Vitro and In Vivo
- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
- Purification of C1q Receptors and Functional Analysis
- Detection of Cell Membrane-bound CD46 using Flow Cytometry
- Detection of CD59 using Flow Cytometry
- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
- Classical Complement Pathway Component C1q? Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q? Derivatives. Functional Characterization
- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
- Analysis of the Complement Activation in Mice
- Opsonophagocytic Assay
- Classical Complement Pathway Components C1r and C1s? Purification from Human Serum and in Recombinant Form and Functional Characterization
- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
- Purification and Characterisation of human and Mouse Complement C3
- Purification of Human Complement Protein C5
- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
- Control code
- 867546768
- Dimensions
- unknown
- Extent
- 1 online resource (xvi, 383 pages)
- File format
- unknown
- Form of item
- online
- Isbn
- 9781627037242
- Level of compression
- unknown
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1007/978-1-62703-724-2
- Other physical details
- illustrations (some color).
- Quality assurance targets
- not applicable
- Reformatting quality
- unknown
- Sound
- unknown sound
- Specific material designation
- remote
- System control number
- (OCoLC)867546768
- Label
- The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
- Antecedent source
- unknown
- Bibliography note
- Includes bibliographical references and index
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Color
- multicolored
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
- Purification and Functional Analysis of Human Properdin
- Purification and Functional Characterization of C4b-binding Protein (C4BP)
- Purification and Functional Characterization of Factor I
- C1 inhibitor? Quantification and Purification
- Purification, Quantification and Functional Analysis of Complement Factor H
- Factor H-related Proteins
- Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
- Evaluation of Complement Function by ELISA
- Anti-Factor H Auto Antibodies Assay
- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
- Functional Analysis of C5a Effector Responses In Vitro and In Vivo
- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
- Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
- Purification of C1q Receptors and Functional Analysis
- Detection of Cell Membrane-bound CD46 using Flow Cytometry
- Detection of CD59 using Flow Cytometry
- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
- Classical Complement Pathway Component C1q? Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q? Derivatives. Functional Characterization
- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
- Analysis of the Complement Activation in Mice
- Opsonophagocytic Assay
- Classical Complement Pathway Components C1r and C1s? Purification from Human Serum and in Recombinant Form and Functional Characterization
- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
- Purification and Characterisation of human and Mouse Complement C3
- Purification of Human Complement Protein C5
- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
- Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
- Control code
- 867546768
- Dimensions
- unknown
- Extent
- 1 online resource (xvi, 383 pages)
- File format
- unknown
- Form of item
- online
- Isbn
- 9781627037242
- Level of compression
- unknown
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.1007/978-1-62703-724-2
- Other physical details
- illustrations (some color).
- Quality assurance targets
- not applicable
- Reformatting quality
- unknown
- Sound
- unknown sound
- Specific material designation
- remote
- System control number
- (OCoLC)867546768
Subject
- Amino Acids, Peptides, and Proteins
- Blood Proteins
- Complement (Immunology)
- Complement (Immunology) -- Laboratory manuals
- Complement Activation
- Complement System Proteins
- Immune System Phenomena
- Immunoproteins
- Laboratory Manuals
- Laboratory manuals
- Membrane Proteins
- Phenomena and Processes
- Proteins
- Publication Characteristics
- Publication Formats
- Receptors, Cell Surface
- Receptors, Complement
- Receptors, Immunologic
Genre
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<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.missouri.edu/portal/The-complement-system--methods-and-protocols/nd1ExAlW49s/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.missouri.edu/portal/The-complement-system--methods-and-protocols/nd1ExAlW49s/">The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.missouri.edu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.missouri.edu/">University of Missouri Libraries</a></span></span></span></span></div>
