Coverart for item
The Resource The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA

The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA

Label
The complement system : methods and protocols
Title
The complement system
Title remainder
methods and protocols
Statement of responsibility
edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
Contributor
Editor
Subject
Genre
Language
eng
Summary
Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols to purify and analyze the activity of the individual complement components or pathways. It includes assays that describe detection of complement SNPs, clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established classical assays and recently developed new assays to analyze the complement system activation will be useful to a wide audience of scientists
Member of
Cataloging source
GW5XE
Dewey number
571.9/688
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QR185.8.C6
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QW 525
NLM item number
ME9616J v.1100 2014
http://library.link/vocab/relatedWorkOrContributorName
Gadjeva, Mihaela
Series statement
Methods in Molecular Biology,
Series volume
1100
http://library.link/vocab/subjectName
  • Complement (Immunology)
  • Immunoproteins
  • Publication Formats
  • Immune System Phenomena
  • Receptors, Immunologic
  • Blood Proteins
  • Receptors, Cell Surface
  • Publication Characteristics
  • Membrane Proteins
  • Phenomena and Processes
  • Proteins
  • Amino Acids, Peptides, and Proteins
  • Complement System Proteins
  • Receptors, Complement
  • Laboratory Manuals
  • Complement Activation
  • Complement (Immunology)
Label
The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
  • Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
  • Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
  • Purification and Functional Analysis of Human Properdin
  • Purification and Functional Characterization of C4b-binding Protein (C4BP)
  • Purification and Functional Characterization of Factor I
  • C1 inhibitor? Quantification and Purification
  • Purification, Quantification and Functional Analysis of Complement Factor H
  • Factor H-related Proteins
  • Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
  • Evaluation of Complement Function by ELISA
  • Anti-Factor H Auto Antibodies Assay
  • Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
  • Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
  • Functional Analysis of C5a Effector Responses In Vitro and In Vivo
  • Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
  • Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
  • Purification of C1q Receptors and Functional Analysis
  • Detection of Cell Membrane-bound CD46 using Flow Cytometry
  • Detection of CD59 using Flow Cytometry
  • Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
  • Classical Complement Pathway Component C1q? Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q? Derivatives. Functional Characterization
  • Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
  • Analysis of the Complement Activation in Mice
  • Opsonophagocytic Assay
  • Classical Complement Pathway Components C1r and C1s? Purification from Human Serum and in Recombinant Form and Functional Characterization
  • Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
  • Purification and Characterisation of human and Mouse Complement C3
  • Purification of Human Complement Protein C5
  • Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
  • Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
Control code
867546768
Dimensions
unknown
Extent
1 online resource (xvi, 383 pages)
File format
unknown
Form of item
online
Isbn
9781627037242
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-724-2
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)867546768
Label
The complement system : methods and protocols, edited by Mihaela Gadjeva, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Harvard University, Boston, MA, USA
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing
  • Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System
  • Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
  • Purification and Functional Analysis of Human Properdin
  • Purification and Functional Characterization of C4b-binding Protein (C4BP)
  • Purification and Functional Characterization of Factor I
  • C1 inhibitor? Quantification and Purification
  • Purification, Quantification and Functional Analysis of Complement Factor H
  • Factor H-related Proteins
  • Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)
  • Evaluation of Complement Function by ELISA
  • Anti-Factor H Auto Antibodies Assay
  • Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
  • Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
  • Functional Analysis of C5a Effector Responses In Vitro and In Vivo
  • Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry
  • Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry
  • Purification of C1q Receptors and Functional Analysis
  • Detection of Cell Membrane-bound CD46 using Flow Cytometry
  • Detection of CD59 using Flow Cytometry
  • Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay
  • Classical Complement Pathway Component C1q? Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q? Derivatives. Functional Characterization
  • Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry
  • Analysis of the Complement Activation in Mice
  • Opsonophagocytic Assay
  • Classical Complement Pathway Components C1r and C1s? Purification from Human Serum and in Recombinant Form and Functional Characterization
  • Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
  • Purification and Characterisation of human and Mouse Complement C3
  • Purification of Human Complement Protein C5
  • Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry
  • Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin
Control code
867546768
Dimensions
unknown
Extent
1 online resource (xvi, 383 pages)
File format
unknown
Form of item
online
Isbn
9781627037242
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-724-2
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)867546768

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