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The Resource The role of the AP-1 adaptor complex in trafficking between the trans-Golgi Network and endosomal system, by Christopher Foote, (electronic resource)

The role of the AP-1 adaptor complex in trafficking between the trans-Golgi Network and endosomal system, by Christopher Foote, (electronic resource)

Creator
Contributor
Summary
In Saccharomyces cerevisiae it is generally accepted that there are two routes for trafficking of proteins from the trans-Golgi network (TGN) to the vacuole. One involves direct transport from the TGN to the vacuole. The second involves transport from the TGN to the prevacuolar compartment (PVC) via GGA coated vesicles, followed by PVC to vacuole transport. We propose that there is a third route. This route entails transit from the TGN to the early endosome (EE), followed by delivery to the PVC and vacuole. In support of an alternative route, the processing kinetics of A(F[arrow]A)-ALP are not affected by mutations in the GGA proteins. This is in contrast to proteins that use the GGA pathway, as their delivery to the vacuole is significantly slowed when GGA function is ablated. Further support of an EE itinerary is the observation that A(F[arrow]A)-ALP co localizes with the lipophilic dye, FM4-64 at a time when the dye is associated with the EE. Disruption of the AP-1 vesicle coat complex leads to an accelerated processing of A(F[arrow]A)-ALP. Appending the region of A(F[arrow]A)-ALP that interacts with AP-1 to Cps1p delays its progress to the vacuole. These results are consistent with a model in which A(F[arrow]A)-ALP passes through the EE in transit to the vacuole. A(F[arrow]A)-ALP physically interacts with AP-1, and this interaction delays its delivery to the vacuole. Data presented in this thesis suggests that in Saccharomyces cerevisiae AP-1 functions as a retrieval mechanism from the EE to the TGN
Language
eng
Work
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (November 7, 2006)
  • Vita
Instance
Label
The role of the AP-1 adaptor complex in trafficking between the trans-Golgi Network and endosomal system
Title
The role of the AP-1 adaptor complex in trafficking between the trans-Golgi Network and endosomal system
Statement of responsibility
by Christopher Foote
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
In Saccharomyces cerevisiae it is generally accepted that there are two routes for trafficking of proteins from the trans-Golgi network (TGN) to the vacuole. One involves direct transport from the TGN to the vacuole. The second involves transport from the TGN to the prevacuolar compartment (PVC) via GGA coated vesicles, followed by PVC to vacuole transport. We propose that there is a third route. This route entails transit from the TGN to the early endosome (EE), followed by delivery to the PVC and vacuole. In support of an alternative route, the processing kinetics of A(F[arrow]A)-ALP are not affected by mutations in the GGA proteins. This is in contrast to proteins that use the GGA pathway, as their delivery to the vacuole is significantly slowed when GGA function is ablated. Further support of an EE itinerary is the observation that A(F[arrow]A)-ALP co localizes with the lipophilic dye, FM4-64 at a time when the dye is associated with the EE. Disruption of the AP-1 vesicle coat complex leads to an accelerated processing of A(F[arrow]A)-ALP. Appending the region of A(F[arrow]A)-ALP that interacts with AP-1 to Cps1p delays its progress to the vacuole. These results are consistent with a model in which A(F[arrow]A)-ALP passes through the EE in transit to the vacuole. A(F[arrow]A)-ALP physically interacts with AP-1, and this interaction delays its delivery to the vacuole. Data presented in this thesis suggests that in Saccharomyces cerevisiae AP-1 functions as a retrieval mechanism from the EE to the TGN
Cataloging source
MUU
http://library.link/vocab/creatorDate
1970-
http://library.link/vocab/creatorName
Foote, Christopher
Degree
Ph. D.
Dissertation year
2005.
Granting institution
University of Missouri-Columbia
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Nothwehr, Steven F.
http://library.link/vocab/subjectName
  • Endosomes
  • Saccharomyces cerevisiae
  • Golgi apparatus
  • Carrier proteins
Target audience
specialized
Label
The role of the AP-1 adaptor complex in trafficking between the trans-Golgi Network and endosomal system, by Christopher Foote, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (November 7, 2006)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
75390851
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System details
Mode of access: World Wide Web
Label
The role of the AP-1 adaptor complex in trafficking between the trans-Golgi Network and endosomal system, by Christopher Foote, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file viewed on (November 7, 2006)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
75390851
Dimensions
unknown
Form of item
electronic
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System details
Mode of access: World Wide Web

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