The Resource The role of the yeast COG3, VPS35, and YDR141C proteins in membrane trafficking, by Paul Bruinsma

The role of the yeast COG3, VPS35, and YDR141C proteins in membrane trafficking, by Paul Bruinsma

Label
The role of the yeast COG3, VPS35, and YDR141C proteins in membrane trafficking
Title
The role of the yeast COG3, VPS35, and YDR141C proteins in membrane trafficking
Statement of responsibility
by Paul Bruinsma
Creator
Subject
Language
eng
Summary
Localization mechanisms for resident trans -Golgi network (TGN) proteins have been shown to involve retrieval from the prevacuolar compartment (PVC) and "slow delivery" to the PVC. Early Golgi residents are known to use retrieval, as well as oligomerization and lipid partitioning, to maintain proper localization. The focus of my thesis is to study the roles of COG3, VPS35 , and YDR141c in the process of Golgi resident protein localization. COG3 , a member of the COG complex, was previously found to be required for efficient ER-to-Golgi trafficking, TGN-resident localization, and Golgi-mediated glycosylation. Consistent with these observations, I found that an Ü-1,6-mannosyltransferase resident of the cis -Golgi (Och1p) was partially mislocalized to the cell surface in cog3 mutants. Mutants which block vesicle fusion with the cell surface demonstrated that Och1p does not normally travel to the cell surface as part of its cellular itinerary. Vacuolar turnover of Och1p was also found to be delayed in a cog3 mutant consistent with the idea that it accumulates on the plasma membrane. Och1p was found to use the AP-3 adaptor-mediated pathway to reach the vacuole. Finally, Och1p was found to be retrieved back to the cis -Golgi directly from the TGN. Another protein involved in cycling of Golgi resident proteins is Vps35p. Vps35p was shown to act at the PVC in retrieval of TGN-resident proteins back to the TGN. Two classes of alleles of VPS35 were generated with unique phenotypes: one class retrieved A-ALP normally while failing to retrieve the CPY receptor Vps10p while the other class failed to retrieve A-ALP while retrieving Vps10p correctly. This observation led to the hypothesis that Vps35p has multiple functional domains with different cargo specificities. The final gene looked at in this study was YDR141c which was discovered in a screen looking for mutants defective in the "slow delivery" mechanism. This transposon insertion mutant exhibited an accelerated rate of transport of a TGN protein, A(F [arrow right] A)-ALP, into the PVC. Three is alleles of YDR141c were generated which surprisingly showed a block in A(F [arrow right] A)-ALP trafficking to the PVC/vacuole. Cells depleted of Ydr141cp destabilized Kex2p, another TGN-resident protein. Finally, Ydr141c was shown to be a peripheral membrane protein which predominantly associates with P200 membranes. Sucrose density centrifugation experiments showed that Ydr141cp cofractionates with TGN and PVC markers. The study of these genes has aided our understanding of Golgi-resident localization
Additional physical form
Also available on the Internet.
Cataloging source
MUU
http://library.link/vocab/creatorDate
1973-
http://library.link/vocab/creatorName
Bruinsma, Paul
Degree
Ph. D.
Dissertation year
2002.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • bibliography
  • theses
http://library.link/vocab/subjectName
  • Golgi apparatus
  • Eukaryotic cells
  • Post-translational modification
  • Proteins
  • Yeast
  • Biological transport
Target audience
specialized
Label
The role of the yeast COG3, VPS35, and YDR141C proteins in membrane trafficking, by Paul Bruinsma
Instantiates
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 177-189)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
52491137
Dimensions
29 cm
Extent
xii, 190 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote
Label
The role of the yeast COG3, VPS35, and YDR141C proteins in membrane trafficking, by Paul Bruinsma
Publication
Note
  • Typescript
  • Vita
Bibliography note
Includes bibliographical references (leaves 177-189)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Control code
52491137
Dimensions
29 cm
Extent
xii, 190 leaves
Media category
computer
Media MARC source
rdamedia
Media type code
c
Other physical details
illustrations
Specific material designation
remote

Library Locations

    • University of Missouri Libraries DepositoryBorrow it
      2908 Lemone Blvd, Columbia, MO, 65211, US
      38.919360 -92.291620
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