Coverart for item
The Resource Therapeutic applications of ribozymes and riboswitches : methods and protocols, edited by Daniel Lafontaine, Audrey Dubé, Department of Biology, Université de Sherbrooke, Sherbrooke, QC, Canada

Therapeutic applications of ribozymes and riboswitches : methods and protocols, edited by Daniel Lafontaine, Audrey Dubé, Department of Biology, Université de Sherbrooke, Sherbrooke, QC, Canada

Label
Therapeutic applications of ribozymes and riboswitches : methods and protocols
Title
Therapeutic applications of ribozymes and riboswitches
Title remainder
methods and protocols
Statement of responsibility
edited by Daniel Lafontaine, Audrey Dubé, Department of Biology, Université de Sherbrooke, Sherbrooke, QC, Canada
Contributor
Editor
Subject
Genre
Language
eng
Summary
In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA
Member of
Cataloging source
GW5XE
Dewey number
572.8/8
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP623.5.C36
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
ME9616J v.1103 2014
http://library.link/vocab/relatedWorkOrContributorName
  • Lafontaine, Daniel
  • Dubé, Audrey
Series statement
Methods in Molecular Biology,
Series volume
1103
http://library.link/vocab/subjectName
  • Catalytic RNA
  • Riboswitches
  • RNA, Messenger
  • Publication Formats
  • RNA, Untranslated
  • Enzymes
  • Regulatory Sequences, Ribonucleic Acid
  • RNA
  • Publication Characteristics
  • Untranslated Regions
  • Enzymes and Coenzymes
  • Regulatory Sequences, Nucleic Acid
  • Nucleic Acids
  • Base Sequence
  • Molecular Structure
  • Nucleic Acids, Nucleotides, and Nucleosides
  • Genetic Structures
  • Genetic Phenomena
  • Biochemical Phenomena
  • Chemical Phenomena
  • Phenomena and Processes
  • RNA, Catalytic
  • Riboswitch
  • Laboratory Manuals
  • Catalytic RNA
  • Riboswitches
Label
Therapeutic applications of ribozymes and riboswitches : methods and protocols, edited by Daniel Lafontaine, Audrey Dubé, Department of Biology, Université de Sherbrooke, Sherbrooke, QC, Canada
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Finding instances of riboswitches and ribozymes by homology search of structured RNA with infernal
  • Structure-based virtual screening for the identification of RNA- binding ligands
  • Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays
  • Discovery of small molecule modifiers of micrornas for the treatment of HCV infection
  • Bacterial flavin mononucleotide riboswitches as targets for flavin analogs
  • Construction and application of riboswitch-based sensors that detect metabolites within bacterial cells
  • Screening assays to identify artificial glmS ribozyme activators
  • Analysis of riboswitch structure and ligand binding using small-angle X-ray scattering (SAXS)
  • Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies
  • Cell internalization SELEX: In vitro selection for molecules that internalize into cells
  • Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites
  • DNA electronic switches based on analyte-responsive aptamers
  • Screening inhibitory potential of anti-HIV RT RNA aptamers
  • Design and evaluation of clinically relevant SOFA-HDV ribozymes targeting HIV RNA
  • Directing RNase P-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells
  • Design and analysis of hammerhead ribozyme activity against an artificial gene target
  • Knockdown strategies for the study of proprotein convertases and proliferation in prostate cancer cells
  • Use of tumor-targeting trans-splicing ribozyme for cancer treatment
  • Characterization of hairpin ribozyme reactions
Control code
870683106
Dimensions
unknown
Extent
1 online resource (xi, 278 pages)
File format
unknown
Form of item
online
Isbn
9781627037297
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-730-3
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)870683106
Label
Therapeutic applications of ribozymes and riboswitches : methods and protocols, edited by Daniel Lafontaine, Audrey Dubé, Department of Biology, Université de Sherbrooke, Sherbrooke, QC, Canada
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Finding instances of riboswitches and ribozymes by homology search of structured RNA with infernal
  • Structure-based virtual screening for the identification of RNA- binding ligands
  • Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays
  • Discovery of small molecule modifiers of micrornas for the treatment of HCV infection
  • Bacterial flavin mononucleotide riboswitches as targets for flavin analogs
  • Construction and application of riboswitch-based sensors that detect metabolites within bacterial cells
  • Screening assays to identify artificial glmS ribozyme activators
  • Analysis of riboswitch structure and ligand binding using small-angle X-ray scattering (SAXS)
  • Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies
  • Cell internalization SELEX: In vitro selection for molecules that internalize into cells
  • Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites
  • DNA electronic switches based on analyte-responsive aptamers
  • Screening inhibitory potential of anti-HIV RT RNA aptamers
  • Design and evaluation of clinically relevant SOFA-HDV ribozymes targeting HIV RNA
  • Directing RNase P-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells
  • Design and analysis of hammerhead ribozyme activity against an artificial gene target
  • Knockdown strategies for the study of proprotein convertases and proliferation in prostate cancer cells
  • Use of tumor-targeting trans-splicing ribozyme for cancer treatment
  • Characterization of hairpin ribozyme reactions
Control code
870683106
Dimensions
unknown
Extent
1 online resource (xi, 278 pages)
File format
unknown
Form of item
online
Isbn
9781627037297
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-730-3
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)870683106

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