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The Resource Transcription factor protocols, edited by Martin J. Tymms

Transcription factor protocols, edited by Martin J. Tymms

Contributor
Summary
In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results. Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today
Language
eng
Work
Publication
Extent
1 online resource (x, 306 pages)
Contents
  • Isolation of target gene promoter/enhancer sequences by whole genome PCR method
  • In vivo footprinting using UV light and ligation-mediated PCR
  • Identification of DNasel hypersensitive sites within nuclei
  • Analysis of in vivo methylation
  • Detection of transcription factor partners with a yease one hybrid screen
  • Inverse PCR (IPCR) for obtaining promoter sequence
  • PCR-directed linker scanning mutagenesis
  • Transfection technologies
  • The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues
  • Optimizing electroporation conditions for the transformation of mammalian cells
  • Calcium phosphate transfection of mammalian cultured cells
  • DEAE-dextran transfection of mammaliam cultured cells
  • Liposome-mediated transfection of mammaliam cells
  • Assays for transcriptional activity based on the luciferase reporter gene
  • Transient transfection of Schneider cells in the study of transcription factors
  • Triplex-forming oligonucleotides and their use in the analysis of gene transcription
  • Expression and purification of histidine-tagged transcription factors
  • Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein
  • Electrophoretic mobility shift assays
  • In vitro promoter analysis using nuclear extracts and G-free cassette vectors
  • In vitro transcription using competitor oligonucleotides to deplete specific transcription factors
  • Computer software of eukaryotic promoter analysis
Isbn
9781592596867
Instance
Label
Transcription factor protocols
Title
Transcription factor protocols
Statement of responsibility
edited by Martin J. Tymms
Contributor
Subject
Genre
Language
eng
Summary
In Transcription Factor Protocols, Martin Tymms has created a powerful compendium of the major techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. With chapters contributed by leading investigators well versed in the methods, this eminently practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Step-by-step instructions, tips about pitfalls to avoid, and extensive procedural notes all help to ensure robust and reproducible results. Transcription Factor Protocols offers both experienced workers and new researchers a vital first-stop reference collection of the core techniques for all those exploring the role of transcription factors in gene regulation today
Member of
Is part of
Action
digitized
Cataloging source
N$T
Dewey number
572.8/845
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP552.T68
LC item number
T727 2000eb
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 130
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 450.2
NLM item number
  • ME9616J v.130 2000
  • T77155 2000
http://library.link/vocab/relatedWorkOrContributorName
Tymms, Martin J
Series statement
Methods in molecular biology
Series volume
v. 130
http://library.link/vocab/subjectName
  • Transcription factors
  • Transcription Factors
  • Sequence Analysis, DNA
  • Transfection
  • SCIENCE
  • Transcription factors
  • Transfection
  • Analyse de séquence d'ADN
  • Facteurs de transcription
  • Méthodes
  • Wetenschappelijke technieken
  • Séquençage des acides nucléiques
  • Facteurs de transcription
  • Transfection
Label
Transcription factor protocols, edited by Martin J. Tymms
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis
Control code
172760011
Dimensions
unknown
Extent
1 online resource (x, 306 pages)
File format
unknown
Form of item
online
Isbn
9781592596867
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/159259686X
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)172760011
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Transcription factor protocols, edited by Martin J. Tymms
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation of target gene promoter/enhancer sequences by whole genome PCR method -- In vivo footprinting using UV light and ligation-mediated PCR -- Identification of DNasel hypersensitive sites within nuclei -- Analysis of in vivo methylation -- Detection of transcription factor partners with a yease one hybrid screen -- Inverse PCR (IPCR) for obtaining promoter sequence -- PCR-directed linker scanning mutagenesis -- Transfection technologies -- The use of particle-mediated gene transfer for the study of promoter activity in somatic tissues -- Optimizing electroporation conditions for the transformation of mammalian cells -- Calcium phosphate transfection of mammalian cultured cells -- DEAE-dextran transfection of mammaliam cultured cells -- Liposome-mediated transfection of mammaliam cells -- Assays for transcriptional activity based on the luciferase reporter gene -- Transient transfection of Schneider cells in the study of transcription factors -- Triplex-forming oligonucleotides and their use in the analysis of gene transcription -- Expression and purification of histidine-tagged transcription factors -- Generation of transcription factors in rabbit reticulocyte lysate depleted of endogenous DNA-binding protein -- Electrophoretic mobility shift assays -- In vitro promoter analysis using nuclear extracts and G-free cassette vectors -- In vitro transcription using competitor oligonucleotides to deplete specific transcription factors -- Computer software of eukaryotic promoter analysis
Control code
172760011
Dimensions
unknown
Extent
1 online resource (x, 306 pages)
File format
unknown
Form of item
online
Isbn
9781592596867
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/159259686X
Other physical details
illustrations.
Quality assurance targets
not applicable
Reformatting quality
unknown
Reproduction note
Electronic reproduction.
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)172760011
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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