Coverart for item
The Resource Transgenic ascidians, Yasunori Sasakura, editor

Transgenic ascidians, Yasunori Sasakura, editor

Label
Transgenic ascidians
Title
Transgenic ascidians
Statement of responsibility
Yasunori Sasakura, editor
Contributor
Editor
Subject
Language
eng
Summary
This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research
Member of
Cataloging source
N$T
Dewey number
571.8
Index
index present
LC call number
QH442.6
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QH 442.6
http://library.link/vocab/relatedWorkOrContributorName
Sasakura, Yasunori
Series statement
Advances in experimental medicine and biology,
Series volume
volume 1029
http://library.link/vocab/subjectName
  • Transgenic organisms
  • Sea squirts
  • Cytology
  • Developmental biology
  • Evolution (Biology)
  • Biology
  • Urochordata
  • Genetic Engineering
  • Transgenes
  • Animals, Genetically Modified
  • SCIENCE / Life Sciences / Anatomy & Physiology
  • Biology
  • Cytology
  • Developmental biology
  • Evolution (Biology)
  • Sea squirts
  • Transgenic organisms
Label
Transgenic ascidians, Yasunori Sasakura, editor
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction / Yasunori Sasakura -- Microinjection of exogenous nucleic acids into eggs: Ciona species / Kenji Kobayashi, Yutaka Satou -- Practical guide for ascidian microinjection: Phallusia mammillata / Hitoyoshi Yasuo, Alex McDougall -- Microinjection of exogenous DNA into eggs of Halocynthia roretzi / Gaku Kumano -- Electroporation in ascidians: history, theory and protocols / Robert W. Zeller -- The use of cis-regulatory DNAs as molecular tools / Kotaro Shimai, Takehiro G. Kusakabe -- Reporter analyses reveal redundant enhancers that confer robustness on cis-regulatory mechanisms / Shigeki Fujiwara, Cristian Cañestro -- investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord / Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio -- Purification of fluorescent labeled cells from dissociated Ciona embryos / Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen -- Germline transgenesis in Ciona / Yasunori Sasakura -- The enhancer trap in Ciona / Yasunori Sasakura -- TALEN-based knockout system / Keita Yoshida, Nicholas Treen -- CRISPR knockouts in Ciona embryos / Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi -- Transgenic Techniques for Investigating Cell Biology During Development / Christina D. Cota -- Cellular processes of notochord formation / William C. Smith -- Morphology and physiology of the ascidian nervous systems and the effectors / Atsuo Nishino
Control code
MUHSLdda1028979451
Dimensions
unknown
Extent
1 online resource.
File format
unknown
Form of item
online
Isbn
9789811075452
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)1028979451
Label
Transgenic ascidians, Yasunori Sasakura, editor
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
Introduction / Yasunori Sasakura -- Microinjection of exogenous nucleic acids into eggs: Ciona species / Kenji Kobayashi, Yutaka Satou -- Practical guide for ascidian microinjection: Phallusia mammillata / Hitoyoshi Yasuo, Alex McDougall -- Microinjection of exogenous DNA into eggs of Halocynthia roretzi / Gaku Kumano -- Electroporation in ascidians: history, theory and protocols / Robert W. Zeller -- The use of cis-regulatory DNAs as molecular tools / Kotaro Shimai, Takehiro G. Kusakabe -- Reporter analyses reveal redundant enhancers that confer robustness on cis-regulatory mechanisms / Shigeki Fujiwara, Cristian Cañestro -- investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord / Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio -- Purification of fluorescent labeled cells from dissociated Ciona embryos / Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen -- Germline transgenesis in Ciona / Yasunori Sasakura -- The enhancer trap in Ciona / Yasunori Sasakura -- TALEN-based knockout system / Keita Yoshida, Nicholas Treen -- CRISPR knockouts in Ciona embryos / Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi -- Transgenic Techniques for Investigating Cell Biology During Development / Christina D. Cota -- Cellular processes of notochord formation / William C. Smith -- Morphology and physiology of the ascidian nervous systems and the effectors / Atsuo Nishino
Control code
MUHSLdda1028979451
Dimensions
unknown
Extent
1 online resource.
File format
unknown
Form of item
online
Isbn
9789811075452
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)1028979451

Library Locations

    • J. Otto Lottes Health Sciences LibraryBorrow it
      1 Hospital Dr, Columbia, MO, 65201, US
      38.939544 -92.328377
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