Coverart for item
The Resource cDNA preparation and characterization, edited by Sherman M. Weissman

cDNA preparation and characterization, edited by Sherman M. Weissman

Label
cDNA preparation and characterization
Title
cDNA preparation and characterization
Statement of responsibility
edited by Sherman M. Weissman
Contributor
Editor
Subject
Genre
Language
eng
Summary
Genomic sequences, now emerging at a rapid rate, are greatly expediting certain aspects of molecular biology. However, in more complex organisms, predicting mRNA structure from genomic sequences can often be difficult. Alternative splicing, the use of alternative promoters, and orphan genes without known analogues can call present difficulties in the predictions of the structure of mRNAs or even in gene detection. Both computational and experimental methods remain useful for recognizing genes and transcript templates, even in sequenced DNA. Methods for producing full-length cDNAs are important for determining the structures of the proteins the mRNA encodes, the positions of promoters, and the considerable regulatory information for translation that may be encoded in the 5' untranslated regions of the mRNA. Methods for studying levels of mRNA and their changes in different physiological circumstances are rapidly evolving, and the information from this area will rival the superabundance of information derived from genomic sequences. In particular, cDNAs can be prepared even from single cells, and this approach has already yielded valuable information in several areas. To the extent that reliable and reproducible information, both quantitative and qualitative, can be generated from very small numbers of cells, there are rather remarkable possibilities for complementing functional and genetic analysis of developmental patterns with descriptions of changes in mRNAs. Dense array analysis promises to be particularly valuable for the rapid expression pattern of known genes, while other methods such as gel display approaches offer the opportunity of discovering unidentified genes or for investigating species whose cDNAs or genomes have not been studied intensively. Knowledge of mRNA structure, genomic location, and patterns of expression must be converted into information of the function of the encoded proteins. Each gene can be the subject of years of intensive study. Nevertheless, a number of methods are being developed that use cDNA to predict properties or permit the selective isolation of cDNAs encoding proteins with certain general properties such as selective isolation of cDNAs encoding proteins with certain general properties such as subcellular location. This volume presents an update of a number of approaches relevant to the areas referred to above. The technology in this field is rapidly evolving and these contributions represent a "snapshot in time" of the number of currently available and useful approaches to the problems referred to above. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences
Member of
Action
digitized
Cataloging source
AZU
Dewey number
572.86
Illustrations
  • illustrations
  • plates
Index
index present
Language note
English
LC call number
QP601
LC item number
.M49 vol. 303
Literary form
non fiction
NAL call number
QP601.M49
NAL item number
v.303
Nature of contents
  • dictionaries
  • bibliography
  • handbooks
NLM call number
  • W1
  • QU 58.5
NLM item number
  • ME9615K v. 303 1999
  • C386 1999
http://library.link/vocab/relatedWorkOrContributorName
Weissman, Sherman M.
Series statement
Methods in enzymology,
Series volume
v. 303
http://library.link/vocab/subjectName
  • DNA
  • Messenger RNA
  • Gene mapping
  • DNA, Complementary
  • Base Sequence
  • Chromosome Mapping
  • DNA
  • DNA, Complementary
  • RNA, Messenger
  • Design Network Switzerland
  • DNA
  • Gene mapping
  • Messenger RNA
  • ADN complémentaire
  • ARN messager
  • Cartographie chromosomique
  • ADN complémentaire
  • DNA
  • Kloneren
  • Genetische manipulatie
  • Biosynthese
  • Biochemische Methode
  • Methode
  • Molekularbiologie
  • cDNS
  • Aufsatzsammlung
  • ADN
  • Chromosomes
  • ARN messagers
  • Liaison génétique
  • ADN complémentaire
  • DNS
Label
cDNA preparation and characterization, edited by Sherman M. Weissman
Instantiates
Publication
Bibliography note
Includes bibliographical references and indexes
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
cDNA preparation -- Gene identification -- Patterns of mRNA expression -- Functional relationship among cDNA translation products
Control code
56578168
Extent
1 online resource (xxxii, 575 pages, 6 pages of unnumbered plates)
Form of item
online
Isbn
9780121822040
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)56578168
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
cDNA preparation and characterization, edited by Sherman M. Weissman
Publication
Bibliography note
Includes bibliographical references and indexes
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
cDNA preparation -- Gene identification -- Patterns of mRNA expression -- Functional relationship among cDNA translation products
Control code
56578168
Extent
1 online resource (xxxii, 575 pages, 6 pages of unnumbered plates)
Form of item
online
Isbn
9780121822040
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
(OCoLC)56578168
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

Library Locations

    • Ellis LibraryBorrow it
      1020 Lowry Street, Columbia, MO, 65201, US
      38.944491 -92.326012
Processing Feedback ...