The Resource cDNA preparation and characterization, edited by Sherman M. Weissman
cDNA preparation and characterization, edited by Sherman M. Weissman
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The item cDNA preparation and characterization, edited by Sherman M. Weissman represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.This item is available to borrow from 1 library branch.
Resource Information
The item cDNA preparation and characterization, edited by Sherman M. Weissman represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in University of Missouri Libraries.
This item is available to borrow from 1 library branch.
- Summary
- Genomic sequences, now emerging at a rapid rate, are greatly expediting certain aspects of molecular biology. However, in more complex organisms, predicting mRNA structure from genomic sequences can often be difficult. Alternative splicing, the use of alternative promoters, and orphan genes without known analogues can call present difficulties in the predictions of the structure of mRNAs or even in gene detection. Both computational and experimental methods remain useful for recognizing genes and transcript templates, even in sequenced DNA. Methods for producing full-length cDNAs are important for determining the structures of the proteins the mRNA encodes, the positions of promoters, and the considerable regulatory information for translation that may be encoded in the 5' untranslated regions of the mRNA. Methods for studying levels of mRNA and their changes in different physiological circumstances are rapidly evolving, and the information from this area will rival the superabundance of information derived from genomic sequences. In particular, cDNAs can be prepared even from single cells, and this approach has already yielded valuable information in several areas. To the extent that reliable and reproducible information, both quantitative and qualitative, can be generated from very small numbers of cells, there are rather remarkable possibilities for complementing functional and genetic analysis of developmental patterns with descriptions of changes in mRNAs. Dense array analysis promises to be particularly valuable for the rapid expression pattern of known genes, while other methods such as gel display approaches offer the opportunity of discovering unidentified genes or for investigating species whose cDNAs or genomes have not been studied intensively. Knowledge of mRNA structure, genomic location, and patterns of expression must be converted into information of the function of the encoded proteins. Each gene can be the subject of years of intensive study. Nevertheless, a number of methods are being developed that use cDNA to predict properties or permit the selective isolation of cDNAs encoding proteins with certain general properties such as selective isolation of cDNAs encoding proteins with certain general properties such as subcellular location. This volume presents an update of a number of approaches relevant to the areas referred to above. The technology in this field is rapidly evolving and these contributions represent a "snapshot in time" of the number of currently available and useful approaches to the problems referred to above. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences
- Language
- eng
- Extent
- 1 online resource (xxxii, 575 pages, 6 pages of unnumbered plates)
- Contents
-
- cDNA preparation
- Gene identification
- Patterns of mRNA expression
- Functional relationship among cDNA translation products
- Isbn
- 9780121822040
- Label
- cDNA preparation and characterization
- Title
- cDNA preparation and characterization
- Statement of responsibility
- edited by Sherman M. Weissman
- Subject
-
- ADN -- Guides, manuels, etc
- ADN complémentaire
- ADN complémentaire -- analyse
- ADN complémentaire -- isolement et purification
- ARN messager -- analyse
- ARN messagers
- Aufsatzsammlung
- Base Sequence
- Biochemische Methode
- Biosynthese
- Cartographie chromosomique -- Méthode
- Chromosome Mapping -- methods
- Chromosomes -- Cartes
- DNA
- DNA
- DNA -- Handbooks, manuals, etc
- DNA -- analysis
- DNA, Complementary -- analysis
- DNA, Complementary -- isolation & purification
- DNS
- Design Network Switzerland
- Gene mapping
- Gene mapping
- Genetische manipulatie
- Handbooks and manuals
- Kloneren
- Liaison génétique
- Messenger RNA
- Messenger RNA
- Methode
- Molekularbiologie
- RNA, Messenger -- analysis
- cDNS
- Language
- eng
- Summary
- Genomic sequences, now emerging at a rapid rate, are greatly expediting certain aspects of molecular biology. However, in more complex organisms, predicting mRNA structure from genomic sequences can often be difficult. Alternative splicing, the use of alternative promoters, and orphan genes without known analogues can call present difficulties in the predictions of the structure of mRNAs or even in gene detection. Both computational and experimental methods remain useful for recognizing genes and transcript templates, even in sequenced DNA. Methods for producing full-length cDNAs are important for determining the structures of the proteins the mRNA encodes, the positions of promoters, and the considerable regulatory information for translation that may be encoded in the 5' untranslated regions of the mRNA. Methods for studying levels of mRNA and their changes in different physiological circumstances are rapidly evolving, and the information from this area will rival the superabundance of information derived from genomic sequences. In particular, cDNAs can be prepared even from single cells, and this approach has already yielded valuable information in several areas. To the extent that reliable and reproducible information, both quantitative and qualitative, can be generated from very small numbers of cells, there are rather remarkable possibilities for complementing functional and genetic analysis of developmental patterns with descriptions of changes in mRNAs. Dense array analysis promises to be particularly valuable for the rapid expression pattern of known genes, while other methods such as gel display approaches offer the opportunity of discovering unidentified genes or for investigating species whose cDNAs or genomes have not been studied intensively. Knowledge of mRNA structure, genomic location, and patterns of expression must be converted into information of the function of the encoded proteins. Each gene can be the subject of years of intensive study. Nevertheless, a number of methods are being developed that use cDNA to predict properties or permit the selective isolation of cDNAs encoding proteins with certain general properties such as selective isolation of cDNAs encoding proteins with certain general properties such as subcellular location. This volume presents an update of a number of approaches relevant to the areas referred to above. The technology in this field is rapidly evolving and these contributions represent a "snapshot in time" of the number of currently available and useful approaches to the problems referred to above. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences
- Action
- digitized
- Cataloging source
- AZU
- Dewey number
- 572.86
- Illustrations
-
- illustrations
- plates
- Index
- index present
- Language note
- English
- LC call number
- QP601
- LC item number
- .M49 vol. 303
- Literary form
- non fiction
- NAL call number
- QP601.M49
- NAL item number
- v.303
- Nature of contents
-
- dictionaries
- bibliography
- handbooks
- NLM call number
-
- W1
- QU 58.5
- NLM item number
-
- ME9615K v. 303 1999
- C386 1999
- http://library.link/vocab/relatedWorkOrContributorName
- Weissman, Sherman M.
- Series statement
- Methods in enzymology,
- Series volume
- v. 303
- http://library.link/vocab/subjectName
-
- DNA
- Messenger RNA
- Gene mapping
- DNA, Complementary
- Base Sequence
- Chromosome Mapping
- DNA
- DNA, Complementary
- RNA, Messenger
- Design Network Switzerland
- DNA
- Gene mapping
- Messenger RNA
- ADN complémentaire
- ARN messager
- Cartographie chromosomique
- ADN complémentaire
- DNA
- Kloneren
- Genetische manipulatie
- Biosynthese
- Biochemische Methode
- Methode
- Molekularbiologie
- cDNS
- Aufsatzsammlung
- ADN
- Chromosomes
- ARN messagers
- Liaison génétique
- ADN complémentaire
- DNS
- Label
- cDNA preparation and characterization, edited by Sherman M. Weissman
- Bibliography note
- Includes bibliographical references and indexes
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- cDNA preparation -- Gene identification -- Patterns of mRNA expression -- Functional relationship among cDNA translation products
- Control code
- 56578168
- Extent
- 1 online resource (xxxii, 575 pages, 6 pages of unnumbered plates)
- Form of item
- online
- Isbn
- 9780121822040
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic reproduction.
- Specific material designation
- remote
- System control number
- (OCoLC)56578168
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
- Label
- cDNA preparation and characterization, edited by Sherman M. Weissman
- Bibliography note
- Includes bibliographical references and indexes
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
- cDNA preparation -- Gene identification -- Patterns of mRNA expression -- Functional relationship among cDNA translation products
- Control code
- 56578168
- Extent
- 1 online resource (xxxii, 575 pages, 6 pages of unnumbered plates)
- Form of item
- online
- Isbn
- 9780121822040
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other physical details
- illustrations (some color).
- Reproduction note
- Electronic reproduction.
- Specific material designation
- remote
- System control number
- (OCoLC)56578168
- System details
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Subject
- ADN -- Guides, manuels, etc
- ADN complémentaire
- ADN complémentaire -- analyse
- ADN complémentaire -- isolement et purification
- ARN messager -- analyse
- ARN messagers
- Aufsatzsammlung
- Base Sequence
- Biochemische Methode
- Biosynthese
- Cartographie chromosomique -- Méthode
- Chromosome Mapping -- methods
- Chromosomes -- Cartes
- DNA
- DNA
- DNA -- Handbooks, manuals, etc
- DNA -- analysis
- DNA, Complementary -- analysis
- DNA, Complementary -- isolation & purification
- DNS
- Design Network Switzerland
- Gene mapping
- Gene mapping
- Genetische manipulatie
- Handbooks and manuals
- Kloneren
- Liaison génétique
- Messenger RNA
- Messenger RNA
- Methode
- Molekularbiologie
- RNA, Messenger -- analysis
- cDNS
Genre
Member of
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