Protocols for nucleic acid analysis by nonradioactive probes

The Resource Protocols for nucleic acid analysis by nonradioactive probes
Label
Protocols for nucleic acid analysis by nonradioactive probes
Statement of responsibility
edited by Peter G. Isaac
Title variation
Nonradioactive probes
Contributor
Subject
Language
eng
Summary
This book provides an exceptionally useful collection of tried and tested protocols for nucleic acid analysis that avoid the use of radioisotopes. Southern (i.e., DNA) and Northern (i.e., RNA) blotting protocols are covered in detail, from how to isolate good quality nucleic acids from plant and animal sources, to the use of the widest range of probe systems (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). Other techniques treated in detail include hybridization in situ to chromosomes and transcribed RNA, newer and less conventional technologies, such as Randomly Amplified Polymorphic DNA (RAPD), reverse dot blots, bacterial identification using magnetic bead and dipstick assays, and nucleic acid sequence-based amplification
Member of
Cataloging source
YUS
Dewey number
574.19/249
Illustrations
illustrations
Index
index present
Language note
English
LC call number
QP620
LC item number
.P765 1994
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Methods in molecular biology
Series volume
v. 28

Context

Context of Protocols for nucleic acid analysis by nonradioactive probes

Work of

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