A novel PCR based DNA microanalyzer system for detection of viral genome

Resource Information

The work A novel PCR based DNA microanalyzer system for detection of viral genome represents a distinct intellectual or artistic creation found in University of Missouri Libraries. This resource is a combination of several types including: Work, Language Material, Books.

Label

A novel PCR based DNA microanalyzer system for detection of viral genome

Statement of responsibility

by Shantanu Bhattacharya

Creator

• Bhattacharya, Shantanu, 1974-

Contributor

• Grant, Sheila Ann
• Gangopadhyay, Shubhra

Thesis advisor

• Grant, Sheila Ann
• Gangopadhyay, Shubhra

Subject

• DNA -- Analysis
• Electronic books
• Electronic dissertations
• Microchemistry
• Polymerase chain reaction
• Dissertations, Academic -- University of Missouri--Columbia -- Biological engineering

Genre

• Electronic books
• Electronic dissertations

Language

eng

Summary

A micro-fluidic assay to quickly analyze microscopic samples of DNA is being developed for field applications. It consists of a micro-PCR chamber, micropumps, and micro-heaters. Additional components of the device include gel electrophoresis microchannels and solid core waveguide fluorescence collectors. The intended analyzer is a micro-fluidic platform that is principally based on the three-step polymerase chain reaction (PCR) mechanism. Currently, all off chip control is executed using a labview code. The micro-pumps, PCR chamber and capillary electrophoresis system have been designed fabricated and tested. For fabrication of the device, a regime has been developed for bonding PDMS surfaces to a variety of substrates (silicon in the present case). We have successfully achieved a compression in the cycle time by a factor of ten in our on chip PCR reactor as compared to the conventional PCR system and also amplify samples with pico-gram concentration. Fluorescent studies indicate negligible non-specific binding to our chip which has been a major problem in earlier assays. A working electrophoretic capillary and new biphasic gel material with extremely low background and high signal to noise ratio have been developed. We have further achieved low voltage capillary electrophoresis by doping different gel materials with conducting nano-particles. We envision this assay as a highly sensitive field deployable analyzer tool

Cataloging source

MUU

Degree

Ph.D.

Dissertation year

2006.

Granting institution

University of Missouri-Columbia

Illustrations

illustrations

Index

no index present

Literary form

non fiction

Nature of contents

• dictionaries
• bibliography
• theses

Target audience

specialized