Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system

Resource Information

The work Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system represents a distinct intellectual or artistic creation found in University of Missouri Libraries. This resource is a combination of several types including: Work, Language Material, Books.

Label

Identification of TEF cofactor(s) in skeletal muscles utilizing yeast two hybrid system

Statement of responsibility

by Aijing Zhang

Creator

• Zhang, Aijing

Subject

• Electronic books.
• Electronic bookss.
• Gene Expression Regulation
• Muscle, Skeletal -- metabolism
• Trans-Activators
• Dissertations, Academic -- University of Missouri--Columbia -- biochemistry (Medicine)
• Transcription Factors -- metabolism
• Two-Hybrid System Techniques
• Transcription Factors -- genetics

Language

eng

Summary

In mouse adult skeletal muscle, Ý-myosin heavy chain (ÝMyHC) gene is primarily expressed in type I slow muscle fibers. However, its expression is down-regulated when the muscle is subjected to non-weight bearing (NWB). The ÝMyHC proximal promoter (Ý293: -293bp to +120bp) has been demonstrated to possess the full activity of the endogenous promoter throughout developmental stages, as well as responsiveness to mechanical overload (MOV) in transgenic studies. Distal muscle CAT element (dMCAT), proximal MCAT (pMCAT) and ÝA/T-rich elements have all been shown to be recognized by TEF-1 (Transcriptional Enhancer Factor-1) proteins, thus demonstrating TEF plays an important role in muscle-specific gene expression and as well as muscle plasticity. There are four members in TEF transcription factor family. Although they recognize the same DNA element, observations suggest that the functions of TEF-1 proteins are not redundant. The unique "squelching" property of TEF-1 in transfection analysis suggests that transactivation activity of TEF-1 requires other limiting and titratable proteins. Protein interactions serve as important mechanisms in transcriptional regulation. It is proposed that the transcriptional specificity of TEF-1 proteins is achieved through the association with different interacting proteins. In this study, utilizing yeast two hybrid screening we obtained two unique clones encoding proteins with known interaction with TEF proteins. The results not only demonstrate the validity of yeast two hybrid experiment in our system setting, but also shed lights on the regulation of TEF-1 transactivation activity

Additional physical form

Also issued on the Internet.

Cataloging source

MMU

Degree

M.S.

Dissertation year

2004.

Government publication

government publication of a state province territory dependency etc

Granting institution

University of Missouri--Columbia

Illustrations

illustrations

Index

no index present

Literary form

non fiction

Nature of contents

• theses
• bibliography